دورية أكاديمية
A new type of 3-D peripheral ultrastructure in Glaucocystis (Glaucocystales, Glaucophyta) as revealed by ultra-high voltage electron microscopy.
| العنوان: | A new type of 3-D peripheral ultrastructure in Glaucocystis (Glaucocystales, Glaucophyta) as revealed by ultra-high voltage electron microscopy. |
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| المؤلفون: | Takahashi T; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan., Nishida T; Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, 7-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan., Saito C; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan., Yasuda H; Research Center for Ultra-High Voltage Electron Microscopy, Osaka University, 7-1 Mihogaoka, Ibaraki, Osaka, 567-0047, Japan., Nozaki H; Department of Biological Sciences, Graduate School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. |
| المصدر: | Journal of phycology [J Phycol] 2016 Jun; Vol. 52 (3), pp. 486-90. Date of Electronic Publication: 2016 Apr 19. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley Periodicals Inc Country of Publication: United States NLM ID: 9882935 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1529-8817 (Electronic) Linking ISSN: 00223646 NLM ISO Abbreviation: J Phycol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2015-> : Malden, MA : Wiley Periodicals Inc. Original Publication: [New York] Phycological Society of America. |
| مواضيع طبية MeSH: | Glaucophyta/*ultrastructure , Protoplasts/*ultrastructure, Freeze Substitution ; Freezing ; Glaucophyta/classification ; Microscopy, Electron, Transmission ; Tomography |
| مستخلص: | The coccoid glaucophyte genus Glaucocystis is characterized by having a thick cell wall, which has to date prohibited examination of the native ultrastructural features of the protoplast periphery. Recently, however, the three-dimensional (3-D) ultrastructure of the protoplast periphery was revealed in two divergent Glaucocystis species, with the world's most powerful ultra-high voltage electron microscope (UHVEM). The two species exhibit morphological diversity in terms of their 3-D ultrastructural features. However, these two types do not seem to encompass actual ultrastructural diversity in the genetically diverse genus Glaucocystis. Here, we report a new type of peripheral 3-D ultrastructure resolved in "G. incrassata" SAG 229-2 cells by 3-D modeling based on UHVEM tomography using high-pressure freezing and freeze-substitution fixation. The plasma membrane and underlying flattened vesicles in "G. incrassata" SAG 229-2 exhibited grooves at intervals of 200-600 nm, and the flattened vesicles often overlapped one another at the protoplast periphery. This 3-D ultrastructure differs from those of the two types previously reported in other species of Glaucocystis. The possibility of classification of Glaucocystis species based on the 3-D ultrastructure of the protoplast periphery is discussed. (© 2016 Phycological Society of America.) |
| فهرسة مساهمة: | Keywords: Glaucocystis; diversity; morphology; tomography; ultra-high voltage electron microscopy; ultrastructure; “Glaucocystis incrassata” SAG 229-2 |
| تواريخ الأحداث: | Date Created: 20160609 Date Completed: 20171226 Latest Revision: 20180104 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/jpy.12412 |
| PMID: | 27273537 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1529-8817 |
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| DOI: | 10.1111/jpy.12412 |