دورية أكاديمية
أعرض في EDS

Wnt/β-catenin pathway transactivates microRNA-150 that promotes EMT of colorectal cancer cells by suppressing CREB signaling.

التفاصيل البيبلوغرافية
العنوان: Wnt/β-catenin pathway transactivates microRNA-150 that promotes EMT of colorectal cancer cells by suppressing CREB signaling.
المؤلفون: Guo YH; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China.; Present address: Guangzhou Quality Supervision and Testing Institute, Guangzhou, P. R. China., Wang LQ; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Li B; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Xu H; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Yang JH; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zheng LS; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Yu P; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zhou AD; Present address: Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA., Zhang Y; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Xie SJ; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Liang ZR; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zhang CM; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zhou H; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Qu LH; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China.
المصدر: Oncotarget [Oncotarget] 2016 Jul 05; Vol. 7 (27), pp. 42513-42526.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Impact Journals Country of Publication: United States NLM ID: 101532965 Publication Model: Print Cited Medium: Internet ISSN: 1949-2553 (Electronic) Linking ISSN: 19492553 NLM ISO Abbreviation: Oncotarget Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Albany, N.Y. : Impact Journals
مواضيع طبية MeSH: Epithelial-Mesenchymal Transition* , Wnt Signaling Pathway*, MicroRNAs/*metabolism , beta Catenin/*metabolism, Animals ; Cell Movement ; Cyclic AMP Response Element-Binding Protein/metabolism ; Disease Progression ; E1A-Associated p300 Protein/metabolism ; Gene Expression Regulation, Neoplastic ; HCT116 Cells ; HEK293 Cells ; Humans ; Lymphoid Enhancer-Binding Factor 1/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Transcriptional Activation ; Transfection
مستخلص: A hallmark of aberrant activation of the Wnt/β-catenin signaling pathway has been observed in most colorectal cancers (CRC), but little is known about the role of non-coding RNAs regulated by this pathway. Here, we found that miR-150 was the most significantly upregulated microRNA responsive to elevated of Wnt/β-catenin signaling activity in both HCT116 and HEK293T cells. Mechanistically, the β-catenin/LEF1 complex binds to the conserved TCF/LEF1-binding element in the miR-150 promoter and thereby transactivates its expression. Enforced expression of miR-150 in HCT116 cell line transformed cells into a spindle shape with higher migration and invasion activity. miR-150 markedly suppressed the CREB signaling pathway by targeting its core transcription factors CREB1 and EP300. Knockdown of CREB1 or EP300 and knockout of CREB1 by CRISPR/Cas9 phenocopied the epithelial-mesenchymal transition (EMT) observed in HCT116 cells in response to miR-150 overexpression. In summary, our data indicate that miR-150 is a novel Wnt effector that may significantly enhance EMT of CRC cells by targeting the CREB signaling pathway.
Competing Interests: The authors declare no conflicts of interest.
References: Nucleic Acids Res. 2010 Sep;38(17):5735-45. (PMID: 20460455)
Am J Cancer Res. 2015 Jan 15;5(2):748-55. (PMID: 25973312)
Proc Natl Acad Sci U S A. 2007 Apr 24;104(17):7080-5. (PMID: 17438277)
Gastroenterology. 2012 Feb;142(2):219-32. (PMID: 22155636)
Oncogene. 2006 Jul 27;25(32):4449-57. (PMID: 16532028)
Clin Cancer Res. 2009 Apr 15;15(8):2583-7. (PMID: 19351775)
Cancer Res. 2003 Apr 15;63(8):1906-13. (PMID: 12702582)
Science. 1998 Sep 4;281(5382):1509-12. (PMID: 9727977)
J Cell Mol Med. 2014 Oct;18(10):2125-34. (PMID: 25230975)
Mol Cell Biochem. 2014 May;390(1-2):19-30. (PMID: 24402783)
Eur J Cancer. 2014 Mar;50(5):1013-24. (PMID: 24456795)
Cell. 2007 Oct 5;131(1):146-59. (PMID: 17923094)
Nucleic Acids Res. 2014 Mar;42(5):2988-98. (PMID: 24335145)
Cell. 2005 Jan 14;120(1):15-20. (PMID: 15652477)
Cancer Sci. 2013 Jan;104(1):48-54. (PMID: 23013135)
Proc Natl Acad Sci U S A. 2011 Nov 29;108(48):19204-9. (PMID: 22080605)
Cell. 2014 Jul 3;158(1):157-70. (PMID: 24976009)
Oncogene. 2012 Jun 14;31(24):2968-78. (PMID: 22020335)
Nat Cell Biol. 2014 Mar;16(3):268-80. (PMID: 24561623)
Nat Genet. 2000 Mar;24(3):300-3. (PMID: 10700188)
J Biol Chem. 2010 Aug 13;285(33):25221-31. (PMID: 20551325)
J Biol Chem. 2009 Oct 2;284(40):27533-43. (PMID: 19651774)
Gut. 2007 Mar;56(3):417-25. (PMID: 16840506)
Biochem Biophys Res Commun. 2009 Sep 18;387(2):376-80. (PMID: 19607815)
Hepatology. 2010 Oct;52(4):1431-42. (PMID: 20842632)
Oncogene. 2005 Dec 15;24(56):8277-90. (PMID: 16116478)
Oncogene. 2012 May 31;31(22):2750-60. (PMID: 21963845)
Cell. 2012 Dec 21;151(7):1595-607. (PMID: 23260145)
Annu Rev Biochem. 1999;68:821-61. (PMID: 10872467)
Curr Biol. 1996 Dec 1;6(12):1664-8. (PMID: 8994831)
PLoS One. 2013 Sep 10;8(9):e75315. (PMID: 24040406)
J Biol Chem. 2010 Jan 1;285(1):409-21. (PMID: 19858184)
Genome Biol. 2010;11(8):R90. (PMID: 20799968)
Biochem Biophys Res Commun. 2010 Feb 12;392(3):340-5. (PMID: 20067763)
Nat Rev Mol Cell Biol. 2011 Mar;12(3):141-51. (PMID: 21346730)
Dev Cell. 2009 Jul;17(1):9-26. (PMID: 19619488)
Pancreas. 2015 Apr;44(3):370-9. (PMID: 25522282)
Int J Oncol. 2005 Dec;27(6):1483-7. (PMID: 16273202)
Oncogene. 2002 Aug 29;21(38):5906-11. (PMID: 12185590)
Exp Cell Res. 2013 Feb 1;319(3):173-84. (PMID: 23211718)
Oncol Rep. 2012 Jan;27(1):129-34. (PMID: 21935578)
Cell Death Differ. 2014 Jun;21(6):978-89. (PMID: 24632949)
Nucleic Acids Res. 2013 Jan 7;41(1):e5. (PMID: 22941648)
FEBS Lett. 2013 Aug 2;587(15):2346-51. (PMID: 23747308)
Proc Natl Acad Sci U S A. 2004 Feb 3;101(5):1273-8. (PMID: 14732695)
J Immunol. 2010 Dec 1;185(11):6413-9. (PMID: 21084670)
PLoS One. 2014 Aug 04;9(8):e103965. (PMID: 25090005)
PLoS One. 2013 Dec 02;8(12):e80707. (PMID: 24312495)
Asian Pac J Cancer Prev. 2014;15(15):6269-73. (PMID: 25124610)
Cell Death Differ. 2013 Dec;20(12):1603-14. (PMID: 24212931)
Trends Neurosci. 2005 Aug;28(8):436-45. (PMID: 15982754)
Cell. 2009 Jan 23;136(2):215-33. (PMID: 19167326)
Int J Oncol. 2012 Feb;40(2):519-26. (PMID: 21956205)
Br J Cancer. 2006 May 8;94(9):1326-32. (PMID: 16622451)
Med Sci Monit. 2014 Oct 07;20:1850-7. (PMID: 25287716)
Cancer Res. 2011 Jan 15;71(2):550-60. (PMID: 21098088)
Mol Carcinog. 2015 Mar;54(3):203-15. (PMID: 24115212)
Cell. 2012 Jun 8;149(6):1192-205. (PMID: 22682243)
EMBO J. 2012 Jun 13;31(12):2670-84. (PMID: 22617420)
Sci China Life Sci. 2013 Oct;56(10):897-906. (PMID: 24008388)
فهرسة مساهمة: Keywords: CREB; EMT; Wnt/β-catenin; colorectal cancer; miR-150
المشرفين على المادة: 0 (CREB1 protein, human)
0 (CTNNB1 protein, human)
0 (CTNNB1 protein, mouse)
0 (Creb1 protein, mouse)
0 (Cyclic AMP Response Element-Binding Protein)
0 (LEF1 protein, human)
0 (Lef1 protein, mouse)
0 (Lymphoid Enhancer-Binding Factor 1)
0 (MIRN150 microRNA, human)
0 (MicroRNAs)
0 (beta Catenin)
EC 2.3.1.48 (E1A-Associated p300 Protein)
EC 2.3.1.48 (EP300 protein, human)
EC 2.3.1.48 (Ep300 protein, mouse)
تواريخ الأحداث: Date Created: 20160611 Date Completed: 20180115 Latest Revision: 20220409
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5173152
DOI: 10.18632/oncotarget.9893
PMID: 27285761
قاعدة البيانات: MEDLINE
الوصف
تدمد:1949-2553
DOI:10.18632/oncotarget.9893