دورية أكاديمية
Wnt/β-catenin pathway transactivates microRNA-150 that promotes EMT of colorectal cancer cells by suppressing CREB signaling.
العنوان: | Wnt/β-catenin pathway transactivates microRNA-150 that promotes EMT of colorectal cancer cells by suppressing CREB signaling. |
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المؤلفون: | Guo YH; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China.; Present address: Guangzhou Quality Supervision and Testing Institute, Guangzhou, P. R. China., Wang LQ; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Li B; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Xu H; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Yang JH; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zheng LS; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Yu P; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zhou AD; Present address: Department of Neurosurgery, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA., Zhang Y; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Xie SJ; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Liang ZR; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zhang CM; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Zhou H; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China., Qu LH; Key Laboratory of Gene Engineering of the Ministry of Education, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, P. R. China. |
المصدر: | Oncotarget [Oncotarget] 2016 Jul 05; Vol. 7 (27), pp. 42513-42526. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Impact Journals Country of Publication: United States NLM ID: 101532965 Publication Model: Print Cited Medium: Internet ISSN: 1949-2553 (Electronic) Linking ISSN: 19492553 NLM ISO Abbreviation: Oncotarget Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Albany, N.Y. : Impact Journals |
مواضيع طبية MeSH: | Epithelial-Mesenchymal Transition* , Wnt Signaling Pathway*, MicroRNAs/*metabolism , beta Catenin/*metabolism, Animals ; Cell Movement ; Cyclic AMP Response Element-Binding Protein/metabolism ; Disease Progression ; E1A-Associated p300 Protein/metabolism ; Gene Expression Regulation, Neoplastic ; HCT116 Cells ; HEK293 Cells ; Humans ; Lymphoid Enhancer-Binding Factor 1/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Transcriptional Activation ; Transfection |
مستخلص: | A hallmark of aberrant activation of the Wnt/β-catenin signaling pathway has been observed in most colorectal cancers (CRC), but little is known about the role of non-coding RNAs regulated by this pathway. Here, we found that miR-150 was the most significantly upregulated microRNA responsive to elevated of Wnt/β-catenin signaling activity in both HCT116 and HEK293T cells. Mechanistically, the β-catenin/LEF1 complex binds to the conserved TCF/LEF1-binding element in the miR-150 promoter and thereby transactivates its expression. Enforced expression of miR-150 in HCT116 cell line transformed cells into a spindle shape with higher migration and invasion activity. miR-150 markedly suppressed the CREB signaling pathway by targeting its core transcription factors CREB1 and EP300. Knockdown of CREB1 or EP300 and knockout of CREB1 by CRISPR/Cas9 phenocopied the epithelial-mesenchymal transition (EMT) observed in HCT116 cells in response to miR-150 overexpression. In summary, our data indicate that miR-150 is a novel Wnt effector that may significantly enhance EMT of CRC cells by targeting the CREB signaling pathway. Competing Interests: The authors declare no conflicts of interest. |
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فهرسة مساهمة: | Keywords: CREB; EMT; Wnt/β-catenin; colorectal cancer; miR-150 |
المشرفين على المادة: | 0 (CREB1 protein, human) 0 (CTNNB1 protein, human) 0 (CTNNB1 protein, mouse) 0 (Creb1 protein, mouse) 0 (Cyclic AMP Response Element-Binding Protein) 0 (LEF1 protein, human) 0 (Lef1 protein, mouse) 0 (Lymphoid Enhancer-Binding Factor 1) 0 (MIRN150 microRNA, human) 0 (MicroRNAs) 0 (beta Catenin) EC 2.3.1.48 (E1A-Associated p300 Protein) EC 2.3.1.48 (EP300 protein, human) EC 2.3.1.48 (Ep300 protein, mouse) |
تواريخ الأحداث: | Date Created: 20160611 Date Completed: 20180115 Latest Revision: 20220409 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC5173152 |
DOI: | 10.18632/oncotarget.9893 |
PMID: | 27285761 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1949-2553 |
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DOI: | 10.18632/oncotarget.9893 |