دورية أكاديمية
Recovery of Toxoplasma gondii DNA in experimentally mummified skin and bones: Prospects for paleoparasitological studies to unveil the origin of toxoplasmosis.
| العنوان: | Recovery of Toxoplasma gondii DNA in experimentally mummified skin and bones: Prospects for paleoparasitological studies to unveil the origin of toxoplasmosis. |
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| المؤلفون: | Leles D; Laboratory of Parasite Molecular Biology, Department of Microbiology and Parasitology, Fluminense Federal University, Niterói, RJ, Brazil. Electronic address: dleles@id.uff.br., Lobo A; Laboratory of Paleoparasitology, Escola Nacional de Saúde Pública/Fiocruz, Rio de Janeiro, RJ, Brazil., Rhodes T; Laboratory of Parasite Molecular Biology, Department of Microbiology and Parasitology, Fluminense Federal University, Niterói, RJ, Brazil., Millar PR; Laboratory of Parasite Molecular Biology, Department of Microbiology and Parasitology, Fluminense Federal University, Niterói, RJ, Brazil., Amendoeira MR; Laboratory of Toxoplasmosis and Other Protozoan Infections, Oswaldo Cruz Institute/Fiocruz, Rio de Janeiro, RJ, Brazil., Araújo A; Laboratory of Paleoparasitology, Escola Nacional de Saúde Pública/Fiocruz, Rio de Janeiro, RJ, Brazil. |
| المصدر: | Experimental parasitology [Exp Parasitol] 2016 Sep; Vol. 168, pp. 51-5. Date of Electronic Publication: 2016 Jun 10. |
| نوع المنشور: | Historical Article; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1090-2449 (Electronic) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | DNA, Protozoan/*isolation & purification , Mummies/*parasitology , Toxoplasma/*genetics , Toxoplasmosis/*history, Animals ; Bone and Bones/parasitology ; History, Ancient ; Humans ; Mice ; Models, Animal ; Skin/parasitology ; Toxoplasma/isolation & purification |
| مستخلص: | Paleoparasitology studies parasite infections by finding the parasites' remains in preserved organic remains such as natural or artificial mummy tissues, skeletons, teeth, and coprolites, among others. However, some currently important infections like toxoplasmosis have not been studied by paleoparasitology. The reasons include this parasite's complex life cycle, the resulting difficulties in locating this protozoan in the intermediate host tissues, and the limitation of coprolite studies to felines, the protozoan's definitive host. The current study thus aimed to produce an experimental model for molecular diagnosis of toxoplasmosis, prioritizing its study in bones and skin, the most abundant materials in archeological collections and sites. The study demonstrated the feasibility of recovering Toxoplasma gondii DNA from desiccated material, including bones and skin, in experimental models both with circulating tachyzoites (RH strain), characteristic of acute infection, and with cysts (ME49 cystogenic strain), characteristic of chronic infection. At present, most individuals with T. gondii infection are in the chronic phase, and the same was probably true in the past. The current study thus expands the odds of finding the parasite in archeological material, enhanced by the nature of the material in which the diagnosis was made. Finding the parasite may help answer questions that are widely debated in the literature on this protozoan's origin (Old World versus New World). In addition, when conditions do not allow ideal storage of samples for molecular tests, the methodology creates the possibility of testing oven-dried samples transported at room temperature. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Ancient DNA; Bones; Diagnosis; Mummies; Toxoplasmosis |
| المشرفين على المادة: | 0 (DNA, Protozoan) |
| تواريخ الأحداث: | Date Created: 20160614 Date Completed: 20170105 Latest Revision: 20170106 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.exppara.2016.06.003 |
| PMID: | 27292545 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1090-2449 |
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| DOI: | 10.1016/j.exppara.2016.06.003 |