دورية أكاديمية
Mycobacterial L-forms are found in cord blood: A potential vertical transmission of BCG from vaccinated mothers.
| العنوان: | Mycobacterial L-forms are found in cord blood: A potential vertical transmission of BCG from vaccinated mothers. |
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| المؤلفون: | Markova N; a Institute of Microbiology, Bulgarian Academy of Sciences , Sofia , Bulgaria., Slavchev G; a Institute of Microbiology, Bulgarian Academy of Sciences , Sofia , Bulgaria., Djerov L; b Medical University , Sofia , Bulgaria., Nikolov A; b Medical University , Sofia , Bulgaria., Dimova T; c Institute of Biology and Immunology of Reproduction , Sofia , Bulgaria. |
| المصدر: | Human vaccines & immunotherapeutics [Hum Vaccin Immunother] 2016 Oct 02; Vol. 12 (10), pp. 2565-2571. Date of Electronic Publication: 2016 Jun 13. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101572652 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2164-554X (Electronic) Linking ISSN: 21645515 NLM ISO Abbreviation: Hum Vaccin Immunother Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Austin, Tex. : Landes Bioscience |
| مواضيع طبية MeSH: | BCG Vaccine/*administration & dosage , Fetal Blood/*microbiology , L Forms/*isolation & purification , Mycobacterium bovis/*isolation & purification, Female ; Healthy Volunteers ; Humans ; Infant, Newborn ; L Forms/genetics ; L Forms/ultrastructure ; Microscopy, Electron, Transmission ; Mycobacterium bovis/genetics ; Mycobacterium bovis/ultrastructure ; Real-Time Polymerase Chain Reaction |
| مستخلص: | Our previous studies showed that mycobacterial L-forms persist in the blood of BCG vaccinated people and that BCG vaccine is able to produce, under appropriate conditions, filterable, self-replicating L-bodies with virus-like size. Because filterability is one of the characteristics of L-forms, considerable interest has been shown in their capacity to cross the maternal-fetal barrier. The current study demonstrated isolation of mycobacterial L-form cultures from umbilical cord blood of 5 healthy newborns of healthy mothers vaccinated previously with BCG. The isolated cultures showed distinctive growth characteristics of cell wall deficient L-form bacteria. Transmission electron microscopy demonstrated presence of L-bodies with extremely small size of 100 nm and revealed morphological transformations, typical for L-forms. IS6110 Real Time PCR assay confirmed that all L-form isolates were of mycobacterial origin and belonged to Mycobacterium tuberculosis complex which includes vaccinal BCG substrains. In conclusion, we could suggest that reproductive filterable L-bodies of BCG origin are able to fall in blood circulation of the fetus by vertical transmitted pathway and colonize newborns. |
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| فهرسة مساهمة: | Keywords: BCG; L-forms; cord blood; mycobacteria; newborns |
| المشرفين على المادة: | 0 (BCG Vaccine) |
| تواريخ الأحداث: | Date Created: 20160614 Date Completed: 20171002 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5085002 |
| DOI: | 10.1080/21645515.2016.1193658 |
| PMID: | 27294392 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 2164-554X |
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| DOI: | 10.1080/21645515.2016.1193658 |