دورية أكاديمية
The expansion of adult stem/progenitor cells and their marker expression fluctuations are linked with pituitary plastic adaptation during gestation and lactancy.
| العنوان: | The expansion of adult stem/progenitor cells and their marker expression fluctuations are linked with pituitary plastic adaptation during gestation and lactancy. |
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| المؤلفون: | Vaca AM; Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre Esq. Enrique Barros, Ciudad Universitaria, Córdoba, Argentina; and., Guido CB; Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre Esq. Enrique Barros, Ciudad Universitaria, Córdoba, Argentina; and., Sosa Ldel V; Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre Esq. Enrique Barros, Ciudad Universitaria, Córdoba, Argentina; and., Nicola JP; Centro de Investigaciones en Bioquímica Clínica e Inmunología, Centro de Investigaciones en Bioquímica Clínica e Inmunología-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Haya de la Torre y Medina Allende, Ciudad Universitaria, Córdoba, Argentina., Mukdsi J; Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre Esq. Enrique Barros, Ciudad Universitaria, Córdoba, Argentina; and., Petiti JP; Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre Esq. Enrique Barros, Ciudad Universitaria, Córdoba, Argentina; and., Torres AI; Centro de Microscopía Electrónica, Instituto de Investigaciones en Ciencias de la Salud-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Haya de la Torre Esq. Enrique Barros, Ciudad Universitaria, Córdoba, Argentina; and atorres@cmefcm.uncor.edu. |
| المصدر: | American journal of physiology. Endocrinology and metabolism [Am J Physiol Endocrinol Metab] 2016 Aug 01; Vol. 311 (2), pp. E367-79. Date of Electronic Publication: 2016 Jun 14. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Physiological Society Country of Publication: United States NLM ID: 100901226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-1555 (Electronic) Linking ISSN: 01931849 NLM ISO Abbreviation: Am J Physiol Endocrinol Metab Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD. : American Physiological Society |
| مواضيع طبية MeSH: | Adult Stem Cells/*cytology , Lactation/*metabolism , Pituitary Gland/*cytology , Pregnancy, Animal/*metabolism , RNA, Messenger/*metabolism, Adult Stem Cells/metabolism ; Adult Stem Cells/ultrastructure ; Animals ; Cell Plasticity ; Female ; Gene Expression Profiling ; Glial Cell Line-Derived Neurotrophic Factor Receptors/genetics ; Glial Cell Line-Derived Neurotrophic Factor Receptors/metabolism ; Homeodomain Proteins/genetics ; Immunohistochemistry ; Laser Capture Microdissection ; Microscopy, Electron, Transmission ; Microscopy, Fluorescence ; Octamer Transcription Factor-3/genetics ; Octamer Transcription Factor-3/metabolism ; Pituitary Gland/metabolism ; Pituitary Gland/ultrastructure ; Pregnancy ; Rats ; Rats, Wistar ; Real-Time Polymerase Chain Reaction ; SOX9 Transcription Factor/genetics ; SOX9 Transcription Factor/metabolism ; SOXB1 Transcription Factors/genetics ; SOXB1 Transcription Factors/metabolism ; Transcription Factor Pit-1/genetics |
| مستخلص: | Extensive evidence has revealed variations in the number of hormone-producing cells in the pituitary gland, which occur under physiological conditions such as gestation and lactancy. It has been proposed that new hormone-producing cells differentiate from stem cells. However, exactly how and when this takes place is not clear. In this work, we used immunoelectron microscopy to identify adult pituitary stem/progenitor cells (SC/P) localized in the marginal zone (MZ), and additionally, we detected GFRa2-, Sox2-, and Sox9-positive cells in the adenoparenchyma (AP) by fluorescence microscopy. Then, we evaluated fluctuations of SC/P mRNA and protein level markers in MZ and AP during gestation and lactancy. An upregulation in stemness markers was shown at term of gestation (AT) in MZ, whereas there were more progenitor cell markers in the middle of gestation and active lactancy. Concerning committed cell markers, we detected a rise in AP at beginning of lactancy (d1L). We performed a BrdU uptake analysis in MZ and AP cells. The highest level of BrdU uptake was observed in MZ AT cells, whereas in AP this was detected in d1L, followed by a decrease in both the MZ and AP. Finally, we detected double immunostaining for BrdU-GFRa2 in MZ AT cells and BrdU-Sox9 in the AP d1L cells. Taken together, we hypothesize that the expansion of the SC/P niche took place mainly in MZ from pituitary rats in AT and d1L. These results suggest that the SC niche actively participates in pituitary plasticity during these reproductive states, contributing to the origin of hormone cell populations. (Copyright © 2016 the American Physiological Society.) |
| فهرسة مساهمة: | Keywords: gestation; lactancy; marginal zone; pituitary gland; stem cell/progenitor markers |
| المشرفين على المادة: | 0 (Gfra2 protein, rat) 0 (Glial Cell Line-Derived Neurotrophic Factor Receptors) 0 (Homeodomain Proteins) 0 (Octamer Transcription Factor-3) 0 (Prophet of Pit-1 protein) 0 (RNA, Messenger) 0 (SOX9 Transcription Factor) 0 (SOXB1 Transcription Factors) 0 (Sox2 protein, rat) 0 (Transcription Factor Pit-1) |
| تواريخ الأحداث: | Date Created: 20160616 Date Completed: 20170621 Latest Revision: 20180220 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1152/ajpendo.00077.2016 |
| PMID: | 27302752 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1522-1555 |
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| DOI: | 10.1152/ajpendo.00077.2016 |