دورية أكاديمية
Detection and molecular characterization of norovirus from oysters implicated in outbreaks in the US.
| العنوان: | Detection and molecular characterization of norovirus from oysters implicated in outbreaks in the US. |
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| المؤلفون: | Woods JW; FDA, Division of Seafood Safety, Gulf Coast Seafood Laboratory, Dauphin Island, AL, 36528, USA. Electronic address: jacquelina.woods@fda.hhs.gov., Calci KR; FDA, Division of Seafood Safety, Gulf Coast Seafood Laboratory, Dauphin Island, AL, 36528, USA., Marchant-Tambone JG; FDA, Division of Seafood Safety, Gulf Coast Seafood Laboratory, Dauphin Island, AL, 36528, USA., Burkhardt W 3rd; FDA, Division of Seafood Safety, Gulf Coast Seafood Laboratory, Dauphin Island, AL, 36528, USA. |
| المصدر: | Food microbiology [Food Microbiol] 2016 Oct; Vol. 59, pp. 76-84. Date of Electronic Publication: 2016 May 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 8601127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9998 (Electronic) Linking ISSN: 07400020 NLM ISO Abbreviation: Food Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Elsevier Original Publication: London ; Orlando : Academic Press, c1984- |
| مواضيع طبية MeSH: | Disease Outbreaks*/prevention & control, Caliciviridae Infections/*epidemiology , Caliciviridae Infections/*virology , Norovirus/*genetics , Norovirus/*isolation & purification , Ostreidae/*virology , Shellfish/*virology, Animals ; Caliciviridae Infections/diagnosis ; Coliphages/genetics ; Feces/virology ; Gastroenteritis/prevention & control ; Gastroenteritis/virology ; Genotype ; Humans ; Mice ; Phylogeny ; RNA, Viral/genetics ; Real-Time Polymerase Chain Reaction ; Sequence Analysis, DNA ; United States/epidemiology |
| مستخلص: | Human noroviruses are the leading cause of non-bacterial shellfish associated gastroenteritis. Here we report on the detection and characterization of norovirus (NoV) in shellfish associated outbreaks. Requests were received from state and federal officials for technical assistance in the analysis of shellfish for NoV and male specific coliphage (MSC; an enteric virus surrogate) during the years 2009 thru 2014. In outbreaks where NoV was detected, genogroup II (GII) levels ranged from 2.4 to 82.0 RT-qPCR U/g of digestive diverticula (DD) while NoV genogroup I (GI) levels ranged from 1.5 to 29.8 RT-qPCR U/g of DD. Murine norovirus extraction efficiencies ranged between 50 and 85%. MSC levels ranged from <6 to 80 PFU/100 g. Phylogenetic analysis of the outbreak sequences revealed strains clustering with GI.8, GI.4, GII.3, GII.4, GII.7, and GII.21. There was 100% homology between the shellfish and clinical strains occurring in 2 of 8 outbreaks. Known shellfish consumption data demonstrated probable infectious particles ingested as low as 12. These investigations demonstrate effective detection, quantification, and characterization of NoV in shellfish associated with illness. (Published by Elsevier Ltd.) |
| فهرسة مساهمة: | Keywords: Molecular characterization; Norovirus; Outbreak; Oysters |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20160705 Date Completed: 20170228 Latest Revision: 20170228 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.fm.2016.05.009 |
| PMID: | 27375246 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9998 |
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| DOI: | 10.1016/j.fm.2016.05.009 |