دورية أكاديمية
Identification of Candida haemulonii Complex Species: Use of ClinProTools(TM) to Overcome Limitations of the Bruker Biotyper(TM), VITEK MS(TM) IVD, and VITEK MS(TM) RUO Databases.
| العنوان: | Identification of Candida haemulonii Complex Species: Use of ClinProTools(TM) to Overcome Limitations of the Bruker Biotyper(TM), VITEK MS(TM) IVD, and VITEK MS(TM) RUO Databases. |
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| المؤلفون: | Grenfell RC; Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil., da Silva Junior AR; Central Laboratory Division - LIM-03, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo São Paulo, Brazil., Del Negro GM; Laboratory of Medical Mycology - LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo São Paulo, Brazil., Munhoz RB; Central Laboratory Division - LIM-03, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo São Paulo, Brazil., Gimenes VM; Laboratory of Medical Mycology - LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo São Paulo, Brazil., Assis DM; Bruker do Brasil Atibaia, Brazil., Rockstroh AC; bioMérieux São Paulo, Brazil., Motta AL; Central Laboratory Division - LIM-03, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo São Paulo, Brazil., Rossi F; Central Laboratory Division - LIM-03, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo São Paulo, Brazil., Juliano L; Department of Biophysics, Escola Paulista de Medicina, Universidade Federal de São Paulo São Paulo, Brazil., Benard G; Laboratory of Medical Mycology - LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo São Paulo, Brazil., de Almeida Júnior JN; Central Laboratory Division - LIM-03, Hospital das Clínicas da Faculdade de Medicina, Universidade de São PauloSão Paulo, Brazil; Laboratory of Medical Mycology - LIM-53, Hospital das Clínicas FMUSP and Instituto de Medicina Tropical de São Paulo, Universidade de São PauloSão Paulo, Brazil. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2016 Jun 16; Vol. 7, pp. 940. Date of Electronic Publication: 2016 Jun 16 (Print Publication: 2016). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Candida haemulonii is now considered a complex of two species and one variety: C. haemulonii sensu stricto, Candida duobushaemulonii and the variety C. haemulonii var. vulnera. Identification (ID) of these species is relevant for epidemiological purposes and for therapeutic management, but the different phenotypic commercial systems are unable to provide correct species ID for these emergent pathogens. Hence, we evaluated the MALDI-TOF MS performance for the ID of C. haemulonii species, analyzing isolates/strains of C. haemulonii complex species, Candida pseudohaemulonii and Candida auris by two commercial platforms, their databases and softwares. To differentiate C. haemulonii sensu sctricto from the variety vulnera, we used the ClinProTools(TM) models and a single-peak analysis with the software FlexAnalysis(TM). The Biotyper(TM) database gave 100% correct species ID for C. haemulonii sensu stricto, C. pseudohaemulonii and C. auris, with 69% of correct species ID for C. duobushaemulonii. Vitek MS(TM) IVD database gave 100% correct species ID for C. haemulonii sensu stricto, misidentifying all C. duobushaemulonii and C. pseudohaemulonii as C. haemulonii, being unable to identify C. auris. The Vitek MS(TM) RUO database needed to be upgraded with in-house SuperSpectra to discriminate C. haemulonii sensu stricto, C. duobushaemulonii, C. pseudohaemulonii, and C. auris strains/isolates. The generic algorithm model from ClinProTools(TM) software showed recognition capability of 100% and cross validation of 98.02% for the discrimination of C. haemulonii sensu stricto from the variety vulnera. Single-peak analysis showed that the peaks 5670, 6878, or 13750 m/z can distinguish C. haemulonii sensu stricto from the variety vulnera. |
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| فهرسة مساهمة: | Keywords: Candida haemulonii complex species; ClinProToolsTM; MALDI-TOF mass spectrometry; Microflex LTTM; Vitek MSTM |
| تواريخ الأحداث: | Date Created: 20160706 Date Completed: 20160706 Latest Revision: 20201001 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC4909767 |
| DOI: | 10.3389/fmicb.2016.00940 |
| PMID: | 27379069 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2016.00940 |