دورية أكاديمية
Functional Recovery in Osteoarthritic Chondrocytes Through Hyaluronic Acid and Platelet-Rich Plasma-Inhibited Infrapatellar Fat Pad Adipocytes.
| العنوان: | Functional Recovery in Osteoarthritic Chondrocytes Through Hyaluronic Acid and Platelet-Rich Plasma-Inhibited Infrapatellar Fat Pad Adipocytes. |
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| المؤلفون: | Chen WH; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan., Lin CM; Department of Neurosurgery, Taipei Medical University-Shuang Ho Hospital, Ministry of Health and Welfare, Taipei, Taiwan., Huang CF; School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan., Hsu WC; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan., Lee CH; Department of Orthopaedics and Traumatology, Orthopaedic Research Center, Taipei Medical University Hospital, Taipei, Taiwan., Ou KL; Research Center for Biomedical Implants and Microsurgery Devices, Taipei, Taiwan Research Center for Biomedical Devices and Prototype Production, Taipei, Taiwan., Dubey NK; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan., Deng WP; Stem Cell Research Center, Taipei Medical University, Taipei, Taiwan Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan College of Medicine, Fu-Jen Catholic University, Taipei, Taiwan wpdeng@tmu.edu.tw. |
| المصدر: | The American journal of sports medicine [Am J Sports Med] 2016 Oct; Vol. 44 (10), pp. 2696-2705. Date of Electronic Publication: 2016 Jul 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Publications Country of Publication: United States NLM ID: 7609541 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-3365 (Electronic) Linking ISSN: 03635465 NLM ISO Abbreviation: Am J Sports Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2004- : Thousand Oaks, CA : Sage Publications Original Publication: Baltimore, Williams & Wilkins. |
| مواضيع طبية MeSH: | Platelet-Rich Plasma*, Chondrocytes/*pathology , Hyaluronic Acid/*therapeutic use , Osteoarthritis, Knee/*physiopathology, Adipocytes/metabolism ; Adipose Tissue/metabolism ; Aged ; Aged, 80 and over ; Cartilage, Articular/pathology ; Coculture Techniques ; Cytokines/metabolism ; Extracellular Matrix/pathology ; Female ; Humans ; Inflammation/pathology ; Knee Joint/physiopathology ; Male |
| مستخلص: | Background: Recent studies have shown evidence that higher adiposity in the infrapatellar fat pad (IFP) induces inflammatory phenotypes in the knee joint and thereby contributes to the development and progression of osteoarthritis (OA). In particular, IFP adipocyte-derived inflammatory cytokines participate in pathological events. Our previous research has already addressed the therapeutic efficacy of hyaluronic acid and platelet-rich plasma (HA+PRP), including the promotion of cartilage regeneration and the inhibition of inflammation. The current study aimed to explore the remedial action of coadministered HA+PRP in OA recovery via IFP adipocyte inhibition. Hypothesis: HA+PRP repairs OA articular cartilage by inhibiting the release of adipokines from IFP adipocytes. Study Design: Controlled laboratory study. Methods: IFP adipocytes and articular chondrocytes were obtained from 10 patients with OA, and the effects of releasates containing cytokines and adipokines in IFP adipocyte-derived conditioned medium (IACM) on articular chondrocytes and IFP adipocytes themselves were evaluated. The therapeutic efficacy of exogenous HA+PRP was determined through its administration to cocultured IFP adipocytes and articular chondrocytes and further demonstrated in a 3-dimensional (3D) arthritic neocartilage model. Results: The IACM and IFP adipocyte-induced microenvironment could induce dedifferentiated and inflammatory phenotypes in articular chondrocytes. HA+PRP decreased the inflammatory potential of IFP adipocytes through the profound inhibition of cytokines and adipokines. The IACM-mediated and -reduced cartilaginous extracellular matrix could also be recovered through HA+PRP in the 3D arthritic neocartilage model. Conclusion: IFP adipocyte-derived releasates mediated inflammatory response dedifferentiation in chondrocytes, which was recovered through HA+PRP administration. Clinical Relevance: Our findings demonstrated that HA+PRP effectively diminished IFP adipocyte-promoted inflammation in articular chondrocytes, indicating that the IFP could be a potential therapeutic target for OA therapy. (© 2016 The Author(s).) |
| فهرسة مساهمة: | Keywords: adipocytes*; hyaluronic acid (HA)*; infrapatellar fat pad (IFP)*; osteoarthritis (OA)*; platelet-rich plasma (PRP)* |
| المشرفين على المادة: | 0 (Cytokines) 9004-61-9 (Hyaluronic Acid) |
| تواريخ الأحداث: | Date Created: 20160713 Date Completed: 20171013 Latest Revision: 20190318 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1177/0363546516651822 |
| PMID: | 27400716 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1552-3365 |
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| DOI: | 10.1177/0363546516651822 |