دورية أكاديمية
A Retrievable, Efficacious Polymeric Scaffold for Subcutaneous Transplantation of Rat Pancreatic Islets.
| العنوان: | A Retrievable, Efficacious Polymeric Scaffold for Subcutaneous Transplantation of Rat Pancreatic Islets. |
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| المؤلفون: | Smink AM; Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands †Polyganics, Groningen, The Netherlands ‡Department of Developmental BioEngineering, Faculty of Science and Technology, University of Twente, Enschede, The Netherlands §Department of Nephrology, Leiden University Medical Center, Leiden, The Netherlands., Hertsig DT, Schwab L, van Apeldoorn AA, de Koning E, Faas MM, de Haan BJ, de Vos P |
| المصدر: | Annals of surgery [Ann Surg] 2017 Jul; Vol. 266 (1), pp. 149-157. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: United States NLM ID: 0372354 Publication Model: Print Cited Medium: Internet ISSN: 1528-1140 (Electronic) Linking ISSN: 00034932 NLM ISO Abbreviation: Ann Surg Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Philadelphia, PA : Lippincott Williams & Wilkins |
| مواضيع طبية MeSH: | Polymers* , Tissue Scaffolds*, Diabetes Mellitus, Experimental/*surgery , Islets of Langerhans Transplantation/*methods, Animals ; Biocompatible Materials ; Cell Culture Techniques ; Cell Survival ; Polyesters ; Polyethylene Glycols ; Sulfones |
| مستخلص: | Objective: We aim on developing a polymeric ectopic scaffold in a readily accessible site under the skin. Summary Background Data: The liver as transplantation site for pancreatic islets is associated with significant loss of islets. Several extrahepatic sites were tested in experimental animals, but many have practical limitations in the clinical setting and do not have the benefit of easy accessibility. Methods and Results: Functional survival of rat islets was tested during 7 days of culture in the presence of poly(D,L-lactide-co-ε-caprolactone) (PDLLCL), poly(ethylene oxide terephthalate)/polybutylene terephthalate (PEOT/PBT) block copolymer, and polysulfone. Tissue responses were studied in vivo after subcutaneous implantation in rats. Culture on PEOT/PBT and polysulfone profoundly disturbed function of islets, and induced severe tissue responses in vivo. Modification of their hydrophilicity did not change the suitability of the polymers. PDLLCL was the only polymer that promoted functional survival of rat islets in vitro and was associated with minor tissue reactions after 28 days. Rat islets were transplanted in the PDLLCL scaffold in a diabetic rat model. Before islet seeding, the scaffold was allowed to engraft for 28 days to allow the tissue response to dampen and to allow blood vessel growth into the device. Islet transplantation into the scaffold resulted in normoglycemia within 3 days and for the duration of the study period of 16 weeks. Conclusions: In conclusion, we found that some polymers such as PEOT/PBT and polysulfone interfere with islet function. PDLLCL is a suitable polymer to create an artificial islet transplantation site under the skin and supports islet survival. |
| المشرفين على المادة: | 0 (Biocompatible Materials) 0 (Polyesters) 0 (Polymers) 0 (Sulfones) 0 (polyethylene oxide-polybutylene terephthalate copolymer) 25135-51-7 (polysulfone P 1700) 3WJQ0SDW1A (Polyethylene Glycols) 70524-20-8 (lactide-caprolactone copolymer) |
| تواريخ الأحداث: | Date Created: 20160719 Date Completed: 20170821 Latest Revision: 20181202 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1097/SLA.0000000000001919 |
| PMID: | 27429018 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1528-1140 |
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| DOI: | 10.1097/SLA.0000000000001919 |