دورية أكاديمية
A three phase hollow fiber liquid-phase microextraction for quantification of lamotrigine in plasma of epileptic patients by capillary electrophoresis.
| العنوان: | A three phase hollow fiber liquid-phase microextraction for quantification of lamotrigine in plasma of epileptic patients by capillary electrophoresis. |
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| المؤلفون: | Barros LS; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Carrão DB; Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., Queiroz RH; Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., de Oliveira AR; Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil., de Gaitani CM; Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil. crisgai@fcfrp.usp.br. |
| المصدر: | Electrophoresis [Electrophoresis] 2016 Oct; Vol. 37 (20), pp. 2678-2684. Date of Electronic Publication: 2016 Sep 22. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: [Weinheim, Germany] : Verlag Chemie, [1980- |
| مواضيع طبية MeSH: | Electrophoresis, Capillary/*methods , Epilepsy/*drug therapy , Liquid Phase Microextraction/*methods , Triazines/*blood, Humans ; Hydrogen-Ion Concentration ; Lamotrigine ; Limit of Detection ; Linear Models ; Reproducibility of Results ; Triazines/therapeutic use |
| مستخلص: | A three phase hollow fiber liquid-phase microextraction technique combined with capillary electrophoresis was developed to quantify lamotrigine (LTG) in plasma samples. The analyte was extracted from 4.0 mL of a basic donor phase (composed of 0.5 mL of plasma and 3.5 mL of sodium phosphate solution pH 9.0) through a supported liquid membrane composed of 1-octanol immobilized in the pores of the hollow fiber, and to an acidic acceptor phase (hydrochloric acid solution pH 4.0) placed in the lumen of the fiber. The extraction was carried out for 30 min at 500 rpm. The eletrophoretic analysis was carried out in 130 mmol/L MES buffer, pH 5.0 with a constant voltage of +15 kV and 20°C. Sample injections were performed for 10 s, at a pressure of 0.5 psi. The detection was performed at 214 nm for both LTG and the internal standard lidocaine. Under the optimized conditions, the method showed a limit of quantification of 1.0 μg/mL and was linear over the plasmatic concentration range of 1.0-20.0 μg/mL. Finally, the validated method was applied for the quantification of LTG in plasma samples of epileptic patients. (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| فهرسة مساهمة: | Keywords: Capillary electrophoresis; Hollow fiber liquid-phase microextraction; Human plasma; Lamotrigine |
| المشرفين على المادة: | 0 (Triazines) U3H27498KS (Lamotrigine) |
| تواريخ الأحداث: | Date Created: 20160727 Date Completed: 20170822 Latest Revision: 20181203 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/elps.201600137 |
| PMID: | 27456073 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1522-2683 |
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| DOI: | 10.1002/elps.201600137 |