دورية أكاديمية
أعرض في EDS

Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures.

التفاصيل البيبلوغرافية
العنوان: Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures.
المؤلفون: Yeo HC; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore.; Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore., Ting S; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore., Brena RM; USC Epigenome Center, University of Southern California, Los Angeles, CA 90033, USA., Koh G; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore., Chen A; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore., Toh SQ; Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore., Lim YM; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore., Oh SK; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore., Lee DY; Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, #06-01, Singapore 138668, Singapore.; Department of Chemical and Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore 117585, Singapore.; NUS Synthetic Biology for Clinical and Technological Innovation (SynCTI), Life Sciences Institute, National University of Singapore, 28 Medical Drive, Singapore 117456, Singapore.
المصدر: Scientific reports [Sci Rep] 2016 Aug 09; Vol. 6, pp. 31068. Date of Electronic Publication: 2016 Aug 09.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Forkhead Transcription Factors/*genetics , Forkhead Transcription Factors/*metabolism , Human Embryonic Stem Cells/*cytology , Human Embryonic Stem Cells/*metabolism , Myocytes, Cardiac/*cytology , Myocytes, Cardiac/*metabolism, Animals ; Binding Sites ; Cell Culture Techniques/methods ; Cell Differentiation/genetics ; Cell Differentiation/physiology ; Cells, Cultured ; Collagen ; Drug Combinations ; Epigenesis, Genetic ; Feeder Cells/metabolism ; Gene Expression Profiling ; Genome-Wide Association Study ; Humans ; Laminin ; Mesoderm/cytology ; Mesoderm/metabolism ; Mice ; Models, Cardiovascular ; Proteoglycans ; Signal Transduction ; Wnt Proteins/metabolism
مستخلص: The differentiation efficiency of human embryonic stem cells (hESCs) into heart muscle cells (cardiomyocytes) is highly sensitive to culture conditions. To elucidate the regulatory mechanisms involved, we investigated hESCs grown on three distinct culture platforms: feeder-free Matrigel, mouse embryonic fibroblast feeders, and Matrigel replated on feeders. At the outset, we profiled and quantified their differentiation efficiency, transcriptome, transcription factor binding sites and DNA-methylation. Subsequent genome-wide analyses allowed us to reconstruct the relevant interactome, thereby forming the regulatory basis for implicating the contrasting differentiation efficiency of the culture conditions. We hypothesized that the parental expressions of FOXC1, FOXD1 and FOXQ1 transcription factors (TFs) are correlative with eventual cardiomyogenic outcome. Through WNT induction of the FOX TFs, we observed the co-activation of WNT3 and EOMES which are potent inducers of mesoderm differentiation. The result strengthened our hypothesis on the regulatory role of the FOX TFs in enhancing mesoderm differentiation capacity of hESCs. Importantly, the final proportions of cells expressing cardiac markers were directly correlated to the strength of FOX inductions within 72 hours after initiation of differentiation across different cell lines and protocols. Thus, we affirmed the relationship between early FOX TF expressions and cardiomyogenesis efficiency.
References: Dev Biol. 2002 Oct 1;250(1):1-23. (PMID: 12297093)
BMC Res Notes. 2010 Aug 06;3:221. (PMID: 20691047)
Cell Stem Cell. 2012 Jan 6;10(1):16-28. (PMID: 22226352)
Cell. 2014 Aug 14;158(4):903-15. (PMID: 25126793)
Cell Stem Cell. 2011 Feb 4;8(2):228-40. (PMID: 21295278)
J Biotechnol. 2006 Mar 9;122(1):130-41. (PMID: 16233925)
J Clin Invest. 2011 Apr;121(4):1313-28. (PMID: 21436589)
Stem Cell Res Ther. 2014 Jan 21;5(1):12. (PMID: 24444355)
Nucleic Acids Res. 2000 Jan 1;28(1):27-30. (PMID: 10592173)
Differentiation. 2010 Jan;79(1):31-40. (PMID: 19782461)
Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17456-61. (PMID: 21987811)
J Cell Biochem. 2008 Jan 1;103(1):182-94. (PMID: 17559079)
Stem Cells. 2008 Jul;26(7):1831-40. (PMID: 18436862)
Methods Mol Biol. 2009;563:123-40. (PMID: 19597783)
Stem Cells. 2008 Jun;26(6):1454-63. (PMID: 18356574)
Cell Stem Cell. 2013 Jun 6;12(6):689-98. (PMID: 23746978)
Nat Biotechnol. 2007 Sep;25(9):1015-24. (PMID: 17721512)
Blood. 2008 Jan 1;111(1):122-31. (PMID: 17875805)
Nature. 2008 May 22;453(7194):524-8. (PMID: 18432194)
Stem Cell Res. 2014 May;12 (3):730-41. (PMID: 24727632)
Database (Oxford). 2010 Aug 05;2010:baq020. (PMID: 20689021)
Adv Exp Med Biol. 2009;665:63-77. (PMID: 20429416)
EMBO J. 1993 Dec;12(12):4898-9. (PMID: 8223498)
J Biotechnol. 2010 Oct 1;150(1):175-81. (PMID: 20633583)
Stem Cells. 2007 Apr;25(4):929-38. (PMID: 17185609)
PLoS Comput Biol. 2010 Oct 14;6(10):e1000956. (PMID: 20976242)
Nucleic Acids Res. 2012 Apr;40(8):3329-47. (PMID: 22199256)
Nat Commun. 2014 Oct 22;5:5288. (PMID: 25335909)
Stem Cells. 2016 Jun;34(6):1487-500. (PMID: 26824887)
PLoS One. 2014 Jan 09;9(1):e85419. (PMID: 24416407)
Science. 2005 Oct 21;310(5747):496-8. (PMID: 16239477)
Epigenetics. 2009 Jul 1;4(5):330-8. (PMID: 19571681)
Biotechnol J. 2014 May;9(5):675-83. (PMID: 24619905)
Curr Protoc Stem Cell Biol. 2012 May;Chapter 1:Unit1D.7. (PMID: 22605644)
FEBS Lett. 2004 Aug 27;573(1-3):83-92. (PMID: 15327980)
Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6638-43. (PMID: 19366677)
J Cell Biol. 2003 Oct 27;163(2):303-14. (PMID: 14581455)
Biophys J. 2013 Aug 20;105(4):1079-89. (PMID: 23972859)
Proc Natl Acad Sci U S A. 2010 Aug 3;107(31):13742-7. (PMID: 20643952)
Nat Commun. 2016 Jan 29;7:10458. (PMID: 26822956)
PLoS One. 2011 Apr 08;6(4):e18293. (PMID: 21494607)
Differentiation. 2008 Apr;76(4):357-70. (PMID: 18021257)
EMBO Rep. 2012 Apr;13(4):355-62. (PMID: 22402664)
Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):E1848-57. (PMID: 22645348)
Stem Cells. 2009 Aug;27(8):1869-78. (PMID: 19544447)
Genes Dev. 2001 Sep 15;15(18):2470-82. (PMID: 11562355)
Development. 2005 Mar;132(5):873-84. (PMID: 15673572)
Genome Res. 2006 Mar;16(3):383-93. (PMID: 16449502)
Development. 1996 Sep;122(9):2769-78. (PMID: 8787751)
Curr Opin Cardiol. 2011 May;26(3):223-9. (PMID: 21451408)
Curr Biol. 2008 Mar 25;18(6):R244-5. (PMID: 18364225)
Acta Naturae. 2013 Oct;5(4):15-21. (PMID: 24455179)
Nat Protoc. 2009;4(1):44-57. (PMID: 19131956)
PLoS One. 2014 Jul 24;9(7):e102873. (PMID: 25057990)
Methods Enzymol. 2006;411:134-93. (PMID: 16939790)
Nature. 2010 Sep 16;467(7313):285-90. (PMID: 20644535)
Eur Heart J. 2011 Apr;32(8):952-62. (PMID: 21367833)
Cell. 2011 Feb 4;144(3):439-52. (PMID: 21295703)
Cell Biol Int. 2006 Oct;30(10):769-76. (PMID: 16831561)
PLoS One. 2011;6(11):e27231. (PMID: 22076139)
Dev Biol. 2004 Mar 1;267(1):14-28. (PMID: 14975714)
PLoS Comput Biol. 2008 Sep 26;4(9):e1000184. (PMID: 18818726)
Biochem Biophys Res Commun. 1994 Nov 30;205(1):497-505. (PMID: 7999070)
EMBO J. 1994 Oct 17;13(20):5002-12. (PMID: 7957066)
Circ Res. 2008 Jan 4;102(1):16-31. (PMID: 18174472)
المشرفين على المادة: 0 (Drug Combinations)
0 (Forkhead Transcription Factors)
0 (Laminin)
0 (Proteoglycans)
0 (Wnt Proteins)
119978-18-6 (matrigel)
9007-34-5 (Collagen)
تواريخ الأحداث: Date Created: 20160810 Date Completed: 20180430 Latest Revision: 20181113
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC4977469
DOI: 10.1038/srep31068
PMID: 27501774
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/srep31068