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Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species.

التفاصيل البيبلوغرافية
العنوان: Essential Oil of Cymbopogon nardus (L.) Rendle: A Strategy to Combat Fungal Infections Caused by Candida Species.
المؤلفون: De Toledo LG; Department of Biological Sciences, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km. 01, Araraquara, 14800-903 São Paulo, Brazil. luciani.gaspar.toledo@gmail.com., Ramos MA; Department of Biological Sciences, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km. 01, Araraquara, 14800-903 São Paulo, Brazil. matheusramos_91@hotmail.com., Spósito L; Department of Biological Sciences, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km. 01, Araraquara, 14800-903 São Paulo, Brazil. lari_sposito@hotmail.com., Castilho EM; Department of Infectious Diseases, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, 15090-000 São Paulo, Brazil. elza.maria.castilho@gmail.com., Pavan FR; Department of Biological Sciences, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km. 01, Araraquara, 14800-903 São Paulo, Brazil. fernandopavan@fcfar.unesp.br., Lopes Éde O; Department of Biological Sciences, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km. 01, Araraquara, 14800-903 São Paulo, Brazil. ericaoliveir@bol.com.br., Zocolo GJ; Brazilian Agricultural Research Corporation, Embrapa Tropical Agroindustry, 60511-110 Fortaleza, Brazil. guilherme.zocolo@embrapa.br., Silva FA; Brazilian Agricultural Research Corporation, Embrapa Tropical Agroindustry, 60511-110 Fortaleza, Brazil. alinynunes@outlook.com., Soares TH; Brazilian Agricultural Research Corporation, Embrapa Tropical Agroindustry, 60511-110 Fortaleza, Brazil. tigressa.rodrigues@embrapa.br., Dos Santos AG; Department of Natural Active Principles and Toxicology, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Araraquara, 14800-903 São Paulo, Brazil. santosag@fcfar.unesp.br., Bauab TM; Department of Biological Sciences, School of Pharmaceutical Sciences, Universidade Estadual Paulista, Rodovia Araraquara-Jaú, km. 01, Araraquara, 14800-903 São Paulo, Brazil. bauabtm@fcfar.unesp.br., De Almeida MT; Department of Infectious Diseases, Faculty of Medicine of São José do Rio Preto, São José do Rio Preto, 15090-000 São Paulo, Brazil. margarete@famerp.br.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2016 Aug 09; Vol. 17 (8). Date of Electronic Publication: 2016 Aug 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Antifungal Agents/*pharmacology , Candida/*drug effects , Cymbopogon/*chemistry , Oils, Volatile/*pharmacology, Antifungal Agents/chemistry ; Gas Chromatography-Mass Spectrometry ; Hyphae/drug effects ; Inhibitory Concentration 50 ; Microbial Sensitivity Tests ; Oils, Volatile/chemistry
مستخلص: Background: The incidence of fungal infections, especially those caused by Candida yeasts, has increased over the last two decades. However, the indicated therapy for fungal control has limitations. Hence, medicinal plants have emerged as an alternative in the search for new antifungal agents as they present compounds, such as essential oils, with important biological effects. Published data demonstrate important pharmacological properties of the essential oil of Cymbopogon nardus (L.) Rendle; these include anti-tumor, anti-nociceptive, and antibacterial activities, and so an investigation of this compound against pathogenic fungi is interesting.
Objective: The aim of this study was to evaluate the chemical composition and biological potential of essential oil (EO) obtained from the leaves of C. nardus focusing on its antifungal profile against Candida species.
Methods: The EO was obtained by hydrodistillation and analyzed by gas chromatography-mass spectrometry (GC-MS). Testing of the antifungal potential against standard and clinical strains was performed by determining the minimal inhibitory concentration (MIC), time-kill, inhibition of Candida albicans hyphae growth, and inhibition of mature biofilms. Additionally, the cytotoxicity was investigated by the IC50 against HepG-2 (hepatic) and MRC-5 (fibroblast) cell lines.
Results: According to the chemical analysis, the main compounds of the EO were the oxygen-containing monoterpenes: citronellal, geranial, geraniol, citronellol, and neral. The results showed important antifungal potential for all strains tested with MIC values ranging from 250 to 1000 μg/mL, except for two clinical isolates of C. tropicalis (MIC > 1000 μg/mL). The time-kill assay showed that the EO inhibited the growth of the yeast and inhibited hyphal formation of C. albicans strains at concentrations ranging from 15.8 to 1000 μg/mL. Inhibition of mature biofilms of strains of C. albicans, C. krusei and C. parapsilosis occurred at a concentration of 10× MIC. The values of the IC50 for the EO were 96.6 μg/mL (HepG-2) and 33.1 μg/mL (MRC-5).
Conclusion: As a major virulence mechanism is attributed to these types of infections, the EO is a promising compound to inhibit Candida species, especially considering its action against biofilm.
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فهرسة مساهمة: Keywords: Candida; Cymbopogon nardus; antifungal activity; essential oil; gas chromatography
المشرفين على المادة: 0 (Antifungal Agents)
0 (Oils, Volatile)
تواريخ الأحداث: Date Created: 20160813 Date Completed: 20170501 Latest Revision: 20181113
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5000650
DOI: 10.3390/ijms17081252
PMID: 27517903
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms17081252