دورية أكاديمية

Genotyping-by-Sequencing to Predict Resistance to Lima Bean Downy Mildew in a Diversity Panel.

التفاصيل البيبلوغرافية
العنوان: Genotyping-by-Sequencing to Predict Resistance to Lima Bean Downy Mildew in a Diversity Panel.
المؤلفون: Mhora TT; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Ernest EG; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Wisser RJ; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Evans TA; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Patzoldt ME; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Gregory NF; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Westhafer SE; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Polson SW; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark., Donofrio NM; First, third, fourth, fifth, sixth, seventh, and ninth authors: Department of Plant and Soil Sciences, University of Delaware, Newark; second author: University of Delaware Research and Education Center, Georgetown; and eighth author: Center for Bioinformatics and Computational Biology, University of Delaware, Newark.
المصدر: Phytopathology [Phytopathology] 2016 Oct; Vol. 106 (10), pp. 1152-1158. Date of Electronic Publication: 2016 Aug 22.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Phytopathological Society] Country of Publication: United States NLM ID: 9427222 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0031-949X (Print) Linking ISSN: 0031949X NLM ISO Abbreviation: Phytopathology Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [St. Paul, Minn., etc., American Phytopathological Society]
مواضيع طبية MeSH: Genotyping Techniques/*methods , Phaseolus/*genetics , Phytophthora/*physiology , Plant Diseases/*immunology, Alleles ; Computational Biology ; Genetic Linkage ; Genetic Markers/genetics ; Genotype ; Haplotypes ; Phaseolus/immunology ; Phaseolus/parasitology ; Phenotype ; Plant Diseases/parasitology ; Seedlings/genetics ; Seedlings/immunology ; Seedlings/parasitology ; Sequence Analysis, DNA
مستخلص: Lima bean is affected by severe downy mildew epidemics caused by the oomycete Phytophthora phaseoli. There are six documented races of P. phaseoli (A to F). Race F is currently predominant in the mid-Atlantic region, creating the need for resistant lima bean cultivars with desirable agronomic characteristics. In order to develop markers for detecting race F resistance, bulked segregant analysis (BSA) using genotyping-by-sequencing (GBS) was used on a biparental F 2 population comprised of 216 lima bean progeny segregating for a dominant race F resistance phenotype. Data were analyzed using a custom bioinformatic analysis pipeline (redrep). Kompetitive allele-specific polymerase chain reaction assays were developed using 12 GBS markers associated with the race F resistance phenotype. Using these assays, the F 2 population was used to map the race F resistance locus. Seven markers were in linkage and significantly associated with race F resistance that mapped between two markers located approximately 4.88 centimorgan (cM) apart. These assays were successfully used to genotype a newly acquired lima bean diversity panel consisting of 256 landraces, cultivars, and wild germplasm, and a haplotype consisting of two of the seven linked markers was demonstrated to accurately predict race F resistance. This confirmed the ability of our customized methods to accurately predict phenotypes in diverse lines of lima bean.
المشرفين على المادة: 0 (Genetic Markers)
تواريخ الأحداث: Date Created: 20160823 Date Completed: 20170629 Latest Revision: 20170629
رمز التحديث: 20240628
DOI: 10.1094/PHYTO-02-16-0087-FI
PMID: 27546812
قاعدة البيانات: MEDLINE
الوصف
تدمد:0031-949X
DOI:10.1094/PHYTO-02-16-0087-FI