دورية أكاديمية
أعرض في EDS

Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro.

التفاصيل البيبلوغرافية
العنوان: Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro.
المؤلفون: Prabhune M; Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany., von Roden K; Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany., Rehfeldt F; Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany., Schmidt CF; Third Institute of Physics-Biophysics, Georg August University, Göttingen, Germany.
المصدر: PloS one [PLoS One] 2016 Aug 25; Vol. 11 (8), pp. e0161623. Date of Electronic Publication: 2016 Aug 25 (Print Publication: 2016).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Maleimides/*chemistry , Microtubules/*drug effects , Paclitaxel/*chemistry, Animals ; Antineoplastic Agents, Phytogenic/chemistry ; Cysteine/chemistry ; Esters ; Microtubules/chemistry ; Polymers/chemistry ; Succinimides/chemistry ; Swine ; Tubulin/chemistry
مستخلص: Microtubule structure and functions have been widely studied in vitro and in cells. Research has shown that cysteines on tubulin play a crucial role in the polymerization of microtubules. Here, we show that blocking sulfhydryl groups of cysteines in taxol-stabilized polymerized microtubules with a commonly used chemical crosslinker prevents temporal end-to-end annealing of microtubules in vitro. This can dramatically affect the length distribution of the microtubules. The crosslinker sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate, sulfo-SMCC, consists of a maleimide and an N-hydroxysuccinimide ester group to bind to sulfhydryl groups and primary amines, respectively. Interestingly, addition of a maleimide dye alone does not show the same interference with annealing in stabilized microtubules. This study shows that the sulfhydryl groups of cysteines of tubulin that are vital for the polymerization are also important for the subsequent annealing of microtubules.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (Antineoplastic Agents, Phytogenic)
0 (Esters)
0 (Maleimides)
0 (Polymers)
0 (Succinimides)
0 (Tubulin)
0 (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
2519R1UGP8 (maleimide)
K848JZ4886 (Cysteine)
MJE3791M4T (N-hydroxysuccinimide)
P88XT4IS4D (Paclitaxel)
تواريخ الأحداث: Date Created: 20160826 Date Completed: 20170807 Latest Revision: 20190212
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC4999061
DOI: 10.1371/journal.pone.0161623
PMID: 27561096
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0161623