دورية أكاديمية
أعرض في EDS

CovR Regulates Streptococcus mutans Susceptibility To Complement Immunity and Survival in Blood.

التفاصيل البيبلوغرافية
العنوان: CovR Regulates Streptococcus mutans Susceptibility To Complement Immunity and Survival in Blood.
المؤلفون: Alves LA; Department of Oral Diagnosis, Piracicaba Dental School-State University of Campinas, Piracicaba, SP, Brazil., Nomura R; Department of Pediatric Dentistry, Osaka University, Graduate School of Dentistry, Osaka, Japan., Mariano FS; Department of Oral Diagnosis, Piracicaba Dental School-State University of Campinas, Piracicaba, SP, Brazil., Harth-Chu EN; Department of Oral Diagnosis, Piracicaba Dental School-State University of Campinas, Piracicaba, SP, Brazil., Stipp RN; Department of Oral Diagnosis, Piracicaba Dental School-State University of Campinas, Piracicaba, SP, Brazil., Nakano K; Department of Pediatric Dentistry, Osaka University, Graduate School of Dentistry, Osaka, Japan., Mattos-Graner RO; Department of Oral Diagnosis, Piracicaba Dental School-State University of Campinas, Piracicaba, SP, Brazil rmgraner@fop.unicamp.br.
المصدر: Infection and immunity [Infect Immun] 2016 Oct 17; Vol. 84 (11), pp. 3206-3219. Date of Electronic Publication: 2016 Oct 17 (Print Publication: 2016).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE
أسماء مطبوعة: Publication: Washington, DC : American Society For Microbiology
Original Publication: [Bethesda, Md.] American Society for Microbiology.
مواضيع طبية MeSH: Bacterial Proteins/*physiology , Complement System Proteins/*immunology , Streptococcus mutans/*physiology, Animals ; Blood/microbiology ; Complement C3b/metabolism ; Complement System Proteins/genetics ; Complement System Proteins/metabolism ; Disease Models, Animal ; Glucans/metabolism ; Humans ; Polysaccharides, Bacterial/metabolism ; Rats ; Rats, Sprague-Dawley ; Streptococcus mutans/pathogenicity ; Transcription, Genetic ; Virulence/physiology
مستخلص: Streptococcus mutans, a major pathogen of dental caries, may promote systemic infections after accessing the bloodstream from oral niches. In this study, we investigate pathways of complement immunity against S. mutans and show that the orphan regulator CovR (CovR Sm ) modulates susceptibility to complement opsonization and survival in blood. S. mutans blood isolates showed reduced susceptibility to C3b deposition compared to oral isolates. Reduced expression of covR Sm in blood strains was associated with increased transcription of CovR Sm -repressed genes required for S. mutans interactions with glucans (gbpC, gbpB, and epsC), sucrose-derived exopolysaccharides (EPS). Consistently, blood strains showed an increased capacity to bind glucan in vitro Deletion of covR Sm in strain UA159 (UAcov) impaired C3b deposition and binding to serum IgG and C-reactive protein (CRP) as well as phagocytosis through C3b/iC3b receptors and killing by neutrophils. Opposite effects were observed in mutants of gbpC, epsC, or gtfBCD (required for glucan synthesis). C3b deposition on UA159 was abolished in C1q-depleted serum, implying that the classical pathway is essential for complement activation on S. mutans Growth in sucrose-containing medium impaired the binding of C3b and IgG to UA159, UAcov, and blood isolates but had absent or reduced effects on C3b deposition in gtfBCD, gbpC, and epsC mutants. UAcov further showed increased ex vivo survival in human blood in an EPS-dependent way. Consistently, reduced survival was observed for the gbpC and epsC mutants. Finally, UAcov showed an increased ability to cause bacteremia in a rat model. These results reveal that CovR Sm modulates systemic virulence by regulating functions affecting S. mutans susceptibility to complement opsonization.
(Copyright © 2016, American Society for Microbiology. All Rights Reserved.)
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المشرفين على المادة: 0 (Bacterial Proteins)
0 (Glucans)
0 (Polysaccharides, Bacterial)
0 (exopolysaccharide, Streptococcus)
80295-43-8 (Complement C3b)
9007-36-7 (Complement System Proteins)
تواريخ الأحداث: Date Created: 20160831 Date Completed: 20170525 Latest Revision: 20181113
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5067753
DOI: 10.1128/IAI.00406-16
PMID: 27572331
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5522
DOI:10.1128/IAI.00406-16