دورية أكاديمية
Garlic activates SIRT-3 to prevent cardiac oxidative stress and mitochondrial dysfunction in diabetes.
| العنوان: | Garlic activates SIRT-3 to prevent cardiac oxidative stress and mitochondrial dysfunction in diabetes. |
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| المؤلفون: | Sultana MR; Department of Medicinal Chemistry and Pharmacology, Indian Institute of Chemical Technology, Hyderabad 500007, India. Electronic address: mdsultana@gmail.com., Bagul PK; Drug Discovery Research Center (DDRC), Translational Health Science and Technology Institute, Faridabad 122001, Haryana, India. Electronic address: pankajbagul2787@gmail.com., Katare PB; Drug Discovery Research Center (DDRC), Translational Health Science and Technology Institute, Faridabad 122001, Haryana, India. Electronic address: pbkatare@gmail.com., Anwar Mohammed S; Drug Discovery Research Center (DDRC), Translational Health Science and Technology Institute, Faridabad 122001, Haryana, India. Electronic address: sohebmsa@gmail.com., Padiya R; Department of Medicinal Chemistry and Pharmacology, Indian Institute of Chemical Technology, Hyderabad 500007, India. Electronic address: rajpadiya@gmail.com., Banerjee SK; Drug Discovery Research Center (DDRC), Translational Health Science and Technology Institute, Faridabad 122001, Haryana, India. Electronic address: skbanerjee@thsti.res.in. |
| المصدر: | Life sciences [Life Sci] 2016 Nov 01; Vol. 164, pp. 42-51. Date of Electronic Publication: 2016 Aug 31. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Garlic* , Heart*/physiology , Oxidative Stress*, Diabetes Complications/*prevention & control , Diabetes Mellitus, Experimental/*diet therapy , Sirtuins/*metabolism, Animals ; Collagen/metabolism ; Immunoblotting ; Mitochondria, Heart/enzymology ; Mitochondria, Heart/pathology ; Oligopeptides/metabolism ; Rats ; Rats, Sprague-Dawley ; Sirtuins/genetics |
| مستخلص: | Background: Cardiac complications are major contributor in the mortality of diabetic people. Mitochondrial dysfunctioning is a crucial contributor for the cardiac complications in diabetes, and SIRT-3 remains the major mitochondrial deacetylase. We hypothesized whether garlic has any role on SIRT-3 to prevent mitochondrial dysfunction in diabetic heart. Methods: Rats with developed hyperglycemia after STZ injection were divided into two groups; diabetic (Dia) and diabetic+garlic (Dia+Garl). Garlic was administered at a dose of 250mg/kg/day, orally for four weeks. An additional group was maintained to evaluate the effect of raw garlic administration on control rat heart. Result: We have observed altered functioning of cardiac mitochondrial enzymes involved in metabolic pathways, and increased levels of cardiac ROS with decreased activity of catalase and SOD in diabetic rats. Cardiac mRNA expression of TFAM, PGC-1α, and CO1 was also altered in diabetes. In addition, reduced levels of electron transport chain complexes that observed in Dia group were normalized with garlic administration. This indicates the presence of increased oxidative stress with mitochondrial dysfunctioning in diabetic heart. We have observed reduced activity of SIRT3 and increased acetylation of MnSOD. Silencing SIRT-3 in cells also revealed the same. However, administration of garlic improved the SIRT-3 and MnSOD activity, by deacetylating MnSOD. Increased SOD activity was correlated with reduced levels of ROS in garlic-administered rat hearts. Conclusion: Collectively, our results provide an insight into garlic's protection to T1DM heart through activation of SIRT3-MnSOD pathway. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Acetylation; Diabetic cardiomyopathy; Garlic; Mitochondria; MnSOD; SIRT3; Sirtuins |
| المشرفين على المادة: | 0 (MHC binding peptide) 0 (Oligopeptides) 0 (SIRT3 protein, rat) 9007-34-5 (Collagen) EC 3.5.1.- (Sirtuins) |
| تواريخ الأحداث: | Date Created: 20160904 Date Completed: 20170203 Latest Revision: 20200827 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.lfs.2016.08.030 |
| PMID: | 27590611 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0631 |
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| DOI: | 10.1016/j.lfs.2016.08.030 |