دورية أكاديمية
أعرض في EDS

Multi-method assessment of patients with febrile illness reveals over-diagnosis of malaria in rural Uganda.

التفاصيل البيبلوغرافية
العنوان: Multi-method assessment of patients with febrile illness reveals over-diagnosis of malaria in rural Uganda.
المؤلفون: Ghai RR; Department of Environmental Sciences, Emory University, Atlanta, GA, 30322, USA. ria.r.ghai@gmail.com.; Department of Biology, McGill University, Montreal, QC, H3A 1B1, Canada. ria.r.ghai@gmail.com., Thurber MI; School of Veterinary Medicine, University of California Davis, 1 Shields Avenue, Davis, CA, 95616, USA., El Bakry A; JD Maclean Centre for Tropical Diseases, McGill University, Montreal General Hospital, Montreal, QC, H3G 1A4, Canada., Chapman CA; Department of Anthropology and McGill School of Environment, McGill University, Montreal, QC, H3A 2T7, Canada.; Makerere University Biological Field Station, PO Box 967, Kampala, Uganda.; Wildlife Conservation Society, 2300 Southern Boulevard, Bronx, NY, 10460, USA., Goldberg TL; Makerere University Biological Field Station, PO Box 967, Kampala, Uganda.; Department of Pathobiological Sciences and Global Health Institute, University of Wisconsin-Madison, Madison, WI, 53706, USA.
المصدر: Malaria journal [Malar J] 2016 Sep 07; Vol. 15, pp. 460. Date of Electronic Publication: 2016 Sep 07.
نوع المنشور: Comparative Study; Evaluation Study; Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101139802 Publication Model: Electronic Cited Medium: Internet ISSN: 1475-2875 (Electronic) Linking ISSN: 14752875 NLM ISO Abbreviation: Malar J Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2002-
مواضيع طبية MeSH: Diagnostic Errors*, Diagnostic Tests, Routine/*methods , Fever of Unknown Origin/*diagnosis , Fever of Unknown Origin/*etiology , Malaria/*diagnosis , Malaria/*epidemiology, Adolescent ; Adult ; Africa ; Aged ; Aged, 80 and over ; Blood/parasitology ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Microscopy ; Middle Aged ; Polymerase Chain Reaction ; Rural Population ; Uganda/epidemiology ; Young Adult
مستخلص: Background: Health clinics in rural Africa are typically resource-limited. As a result, many patients presenting with fever are treated with anti-malarial drugs based only on clinical presentation. This is a considerable issue in Uganda, where malaria is routinely over-diagnosed and over-treated, constituting a wastage of resources and an elevated risk of mortality in wrongly diagnosed patients. However, rapid diagnostic tests (RDTs) for malaria are increasingly being used in health facilities. Being fast, easy and inexpensive, RDTs offer the opportunity for feasible diagnostic capacity in resource-limited areas. This study evaluated the rate of malaria misdiagnosis and the accuracy of RDTs in rural Uganda, where presumptive diagnosis still predominates. Specifically, the diagnostic accuracy of "gold standard" methods, microscopy and PCR, were compared to the most feasible method, RDTs.
Methods: Patients presenting with fever at one of two health clinics in the Kabarole District of Uganda were enrolled in this study. Blood was collected by finger prick and used to administer RDTs, make blood smears for microscopy, and blot Whatman FTA cards for DNA extraction, polymerase chain reaction (PCR) amplification, and sequencing. The accuracy of RDTs and microscopy were assessed relative to PCR, considered the new standard of malaria diagnosis.
Results: A total of 78 patients were enrolled, and 31 were diagnosed with Plasmodium infection by at least one method. Comparing diagnostic pairs determined that RDTs and microscopy performed similarly, being 92.6 and 92.0 % sensitive and 95.5 and 94.4 % specific, respectively. Combining both methods resulted in a sensitivity of 96.0 % and specificity of 100 %. However, both RDTs and microscopy missed one case of non-falciparum malaria (Plasmodium malariae) that was identified and characterized by PCR and sequencing. In total, based on PCR, 62.0 % of patients would have been misdiagnosed with malaria if symptomatic diagnosis was used.
Conclusions: Results suggest that diagnosis of malaria based on symptoms alone appears to be highly inaccurate in this setting. Furthermore, RDTs were very effective at diagnosing malaria, performing as well or better than microscopy. However, only PCR and DNA sequencing detected non-P. falciparum species, which highlights an important limitation of this test and a treatment concern for non-falciparum malaria patients. Nevertheless, RDTs appear the only feasible method in rural or resource-limited areas, and therefore offer the best way forward in malaria management in endemic countries.
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معلومات مُعتمدة: R01 TW009237 United States TW FIC NIH HHS; T35 OD011078 United States OD NIH HHS
فهرسة مساهمة: Keywords: Malaria; Misdiagnosis; Plasmodium; Rapid diagnostic tests; Uganda
تواريخ الأحداث: Date Created: 20160909 Date Completed: 20170626 Latest Revision: 20191210
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC5015337
DOI: 10.1186/s12936-016-1502-4
PMID: 27604542
قاعدة البيانات: MEDLINE
الوصف
تدمد:1475-2875
DOI:10.1186/s12936-016-1502-4