دورية أكاديمية
Genetic Studies of Vibrio cholerae in South West Cameroon-A Phylogenetic Analysis of Isolates from the 2010-2011 Epidemic.
| العنوان: | Genetic Studies of Vibrio cholerae in South West Cameroon-A Phylogenetic Analysis of Isolates from the 2010-2011 Epidemic. |
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| المؤلفون: | Ngwa MC; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA., Masalla T; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Esemu S; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Fumoloh FF; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Kracalik I, Cella E; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA., Alam MT; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA., Akoachere JF; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon., Liang S; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA., Salemi M; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA., Morris JG; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA; Department of Medicine, College of Medicine, University of Florida, Gainesville, Florida, USA., Ali A; Emerging Pathogens Institute, University of Florida, Gainesville, Florida, USA; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, Florida, USA., Ndip LM; Laboratory for Emerging Infectious Diseases, University of Buea, Buea, South West Region, Cameroon; Department of Microbiology, University of Buea, Buea, South West Region, Cameroon. |
| المصدر: | PLoS currents [PLoS Curr] 2016 Aug 12; Vol. 8. Date of Electronic Publication: 2016 Aug 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Public Library of Science Country of Publication: United States NLM ID: 101515638 Publication Model: Electronic Cited Medium: Internet ISSN: 2157-3999 (Electronic) Linking ISSN: 21573999 NLM ISO Abbreviation: PLoS Curr Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: San Francisco, CA : Public Library of Science, 2009-2018 |
| مستخلص: | Introduction: During the cholera outbreak from 2010 to 2011 in Cameroon, 33,192 cases with 1,440 deaths (case fatality ratio 4.34%) were reported to the World Health Organization. Of these, the South West Region reported 3,120 clinical cases. This region is in the Equatorial Monsoon climatic subzone of Cameroon, close to the coast, raising questions as to whether cases were linked with development of environmental reservoirs. Methods: In an investigation conducted by the Laboratory for Emerging Infectious Diseases, University of Buea, toxigenic V. cholerae O1 were isolated from diarrheal stool samples from 18 patients, with ages ranging from <3 to 70 years. Coordinates for clinical centers at which cases were identified were obtained using a handheld GPS, and were mapped using ArcGIS. Antibiotic susceptibility testing was performed using the Kirby 'Bauer agar disc diffusion method. The full genomes of these strains were sequenced with the Illumina MiSeq platform. De novo assembly of cholera genomes and multiple sequence alignment were carried out using the bioinformatics pipeline developed in the Emerging Pathogens Institute laboratory at the University of Florida. Results/discussion: Genetic comparisons showed that isolates were closely related, with pairwise p-distances ranging from 2.25 to 14.52 10-5 nt substitutions per site, and no statistically significant correlation between the pairwise genetic distances and the geographic distances among sampling locations. Indeed, the phylogeny of the Cameroonian strains displays the typical star-like topology and intermixing of strains from different locations that are characteristic of an exponential outbreak localized around a relatively restricted area with occasional spillover to other parts of the country, likely mediated by direct human contact and human movement. Findings highlight the utility of whole genome sequencing and phylogenetic analysis in understanding transmission patterns at the local level. |
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| معلومات مُعتمدة: | R01 AI097405 United States AI NIAID NIH HHS |
| تواريخ الأحداث: | Date Created: 20160913 Date Completed: 20160912 Latest Revision: 20240325 |
| رمز التحديث: | 20240325 |
| مُعرف محوري في PubMed: | PMC5001702 |
| DOI: | 10.1371/currents.outbreaks.13b4e5e36a5c0831a1663fbdb5713fe9 |
| PMID: | 27617169 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2157-3999 |
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| DOI: | 10.1371/currents.outbreaks.13b4e5e36a5c0831a1663fbdb5713fe9 |