دورية أكاديمية
أعرض في EDS

Genome-Wide Assessment of Efficiency and Specificity in CRISPR/Cas9 Mediated Multiple Site Targeting in Arabidopsis.

التفاصيل البيبلوغرافية
العنوان: Genome-Wide Assessment of Efficiency and Specificity in CRISPR/Cas9 Mediated Multiple Site Targeting in Arabidopsis.
المؤلفون: Peterson BA; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Department of Biological Sciences, Virginia Tech, Blacksburg, VA, United States of America., Haak DC; Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech, Blacksburg, VA, United States of America., Nishimura MT; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America., Teixeira PJ; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America., James SR; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Department of Biological Sciences, Virginia Tech, Blacksburg, VA, United States of America., Dangl JL; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Curriculum in Genetics and Molecular Biology, Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Howard Hughes Medical Institute, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America., Nimchuk ZL; Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Curriculum in Genetics and Molecular Biology, Carolina Center for Genome Sciences, University of North Carolina at Chapel Hill, Chapel Hill, NC, United States of America.; Department of Biological Sciences, Virginia Tech, Blacksburg, VA, United States of America.
المصدر: PloS one [PLoS One] 2016 Sep 13; Vol. 11 (9), pp. e0162169. Date of Electronic Publication: 2016 Sep 13 (Print Publication: 2016).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Arabidopsis/*genetics , CRISPR-Cas Systems/*genetics , Gene Targeting/*methods, Genetic Loci/genetics ; Genome, Plant/genetics ; Genome-Wide Association Study ; Translocation, Genetic/genetics
مستخلص: Simultaneous multiplex mutation of large gene families using Cas9 has the potential to revolutionize agriculture and plant sciences. The targeting of multiple genomic sites at once raises concerns about the efficiency and specificity in targeting. The model Arabidopsis thaliana is widely used in basic plant research. Previous work has suggested that the Cas9 off-target rate in Arabidopsis is undetectable. Here we use deep sequencing on pooled plants simultaneously targeting 14 distinct genomic loci to demonstrate that multiplex targeting in Arabidopsis is highly specific to on-target sites with no detectable off-target events. In addition, chromosomal translocations are extremely rare. The high specificity of Cas9 in Arabidopsis makes this a reliable method for clean mutant generation with no need to enhance specificity or adopt alternate Cas9 variants.
Competing Interests: The authors have declared that no competing interests exist.
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تواريخ الأحداث: Date Created: 20160914 Date Completed: 20170804 Latest Revision: 20220408
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC5021288
DOI: 10.1371/journal.pone.0162169
PMID: 27622539
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0162169