دورية أكاديمية
أعرض في EDS

Is Low Non-Lethal Concentration of Methylmercury Really Safe? A Report on Genotoxicity with Delayed Cell Proliferation.

التفاصيل البيبلوغرافية
العنوان: Is Low Non-Lethal Concentration of Methylmercury Really Safe? A Report on Genotoxicity with Delayed Cell Proliferation.
المؤلفون: Crespo-Lopez ME; Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Costa-Malaquias A; Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Oliveira EH; Laboratório de Cultura de Tecidos e Citogenética, Departamento de Meio Ambiente, Instituto Evandro Chagas, 67030-000 Ananindeua (Pará), Brasil., Miranda MS; Laboratório de Fertilização In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Arrifano GP; Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Souza-Monteiro JR; Laboratório de Farmacologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Sagica FE; Laboratório de Cultura de Tecidos e Citogenética, Departamento de Meio Ambiente, Instituto Evandro Chagas, 67030-000 Ananindeua (Pará), Brasil., Fontes-Junior EA; Laboratório de Farmacologia da Inflamação e do Comportamento, Instituto de Ciências da Saúde, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Maia CS; Laboratório de Farmacologia da Inflamação e do Comportamento, Instituto de Ciências da Saúde, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., Macchi BM; Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil., do Nascimento JL; Laboratório de Neuroquímica Molecular e Celular, Instituto de Ciências Biológicas, Universidade Federal do Pará, 66075-110 Belém (Pará), Brasil.
المصدر: PloS one [PLoS One] 2016 Sep 13; Vol. 11 (9), pp. e0162822. Date of Electronic Publication: 2016 Sep 13 (Print Publication: 2016).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Methylmercury Compounds/*toxicity , Mutagens/*toxicity, Animals ; Apoptosis/drug effects ; Cell Cycle/drug effects ; Cell Line ; Cell Proliferation/drug effects ; Cell Survival/drug effects ; DNA Damage ; Humans ; Methylmercury Compounds/administration & dosage ; Mutagenicity Tests ; Mutagens/administration & dosage ; Neuroglia/drug effects ; Neuroglia/metabolism ; Neuroglia/pathology ; Rats
مستخلص: Human exposure to relatively low levels of methylmercury is worrying, especially in terms of its genotoxicity. It is currently unknown as to whether exposure to low levels of mercury (below established limits) is safe. Genotoxicity was already shown in lymphocytes, but studies with cells of the CNS (as the main target organ) are scarce. Moreover, disturbances in the cell cycle and cellular proliferation have previously been observed in neuronal cells, but no data are presently available for glial cells. Interestingly, cells of glial origin accumulate higher concentrations of methylmercury than those of neuronal origin. Thus, the aim of this work was to analyze the possible genotoxicity and alterations in the cell cycle and cell proliferation of a glioma cell line (C6) exposed to a low, non-lethal and non-apoptotic methylmercury concentration. Biochemical (mitochondrial activity) and morphological (integrity of the membrane) assessments confirmed the absence of cell death after exposure to 3 μM methylmercury for 24 hours. Even without promoting cell death, this treatment significantly increased genotoxicity markers (DNA fragmentation, micronuclei, nucleoplasmic bridges and nuclear buds). Changes in the cell cycle profile (increased mitotic index and cell populations in the S and G2/M phases) were observed, suggesting arrest of the cycle. This delay in the cycle was followed, 24 hours after methylmercury withdrawal, by a decrease number of viable cells, reduced cellular confluence and increased doubling time of the culture. Our work demonstrates that exposure to a low sublethal concentration of MeHg considered relatively safe according to current limits promotes genotoxicity and disturbances in the proliferation of cells of glial origin with sustained consequences after methylmercury withdrawal. This fact becomes especially important, since this cellular type accumulates more methylmercury than neurons and displays a vital role protecting the CNS, especially in chronic intoxication with this heavy metal.
Competing Interests: The authors have declared that no competing interests exist.
References: Environ Health Perspect. 2012 Jun;120(6):799-806. (PMID: 22275730)
Environ Res. 2000 Nov;84(3):211-8. (PMID: 11097794)
JAMA. 2016 Feb 2;315(5):489-97. (PMID: 26836731)
Metallomics. 2014 Mar;6(3):662-71. (PMID: 24549367)
Brain Res. 2010 Nov 4;1359:272-80. (PMID: 20813099)
Environ Int. 2007 Feb;33(2):141-6. (PMID: 17007929)
Curr Protoc Immunol. 2001 May;Appendix 3:Appendix 3B. (PMID: 18432654)
Toxicol Appl Pharmacol. 1994 Jul;127(1):83-90. (PMID: 8048057)
Environ Int. 2011 Jan;37(1):136-41. (PMID: 20825993)
Chem Biol Interact. 2011 Sep 5;193(2):129-40. (PMID: 21703249)
Dev Neurobiol. 2013 Dec;73(12):936-49. (PMID: 23959606)
Environ Int. 2001 Oct;27(4):285-90. (PMID: 11686639)
Methods Mol Biol. 2011;731:219-36. (PMID: 21516411)
Neurotoxicology. 2003 Aug;24(4-5):617-23. (PMID: 12900074)
Neurotoxicology. 2005 Mar;26(2):149-57. (PMID: 15713336)
Rev Neurol. 2005 Apr 1-15;40(7):441-7. (PMID: 15849680)
Free Radic Res. 2009 Mar;43(3):224-33. (PMID: 19191107)
Mutagenesis. 2003 Mar;18(2):187-94. (PMID: 12621075)
PLoS One. 2014 Oct 17;9(10):e110815. (PMID: 25330195)
Exp Cell Res. 1988 Mar;175(1):184-91. (PMID: 3345800)
Neurotoxicology. 2006 Dec;27(6):970-81. (PMID: 17056119)
Toxicol Appl Pharmacol. 1999 Nov 1;160(3):279-88. (PMID: 10544062)
Neurotoxicol Teratol. 1990 May-Jun;12(3):191-202. (PMID: 2196419)
Toxicol Sci. 2012 Dec;130(2):383-90. (PMID: 22918959)
J Intern Med. 2013 May;273(5):490-7. (PMID: 23600401)
Environ Sci Pollut Res Int. 2014 Jun;21(12):7466-79. (PMID: 24590602)
Environ Int. 2007 Jan;33(1):56-61. (PMID: 16930706)
Toxicology. 2010 Apr 11;270(2-3):121-30. (PMID: 20149836)
J Immunol Methods. 1983 Dec 16;65(1-2):55-63. (PMID: 6606682)
J Cancer Prev. 2014 Dec;19(4):273-8. (PMID: 25574462)
Chem Biol Interact. 2010 Nov 5;188(2):301-8. (PMID: 20399200)
Toxicol Appl Pharmacol. 2002 Jan 15;178(2):117-25. (PMID: 11814332)
Mutagenesis. 2011 Jan;26(1):125-32. (PMID: 21164193)
Free Radic Biol Med. 2001 Jun 15;30(12):1347-56. (PMID: 11390179)
Environ Int. 2010 Aug;36(6):593-608. (PMID: 20483161)
J Neurosci Res. 2002 Dec 1;70(5):703-11. (PMID: 12424738)
Pharmacol Res. 2009 Oct;60(4):212-20. (PMID: 19446469)
Neurotoxicol Teratol. 2015 Jan-Feb;47:102-13. (PMID: 25496965)
Int J Radiat Oncol Biol Phys. 2004 Oct 1;60(2):591-7. (PMID: 15380596)
Neurotoxicology. 2001 Jun;22(3):317-27. (PMID: 11456333)
المشرفين على المادة: 0 (Methylmercury Compounds)
0 (Mutagens)
تواريخ الأحداث: Date Created: 20160914 Date Completed: 20170731 Latest Revision: 20181113
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5021279
DOI: 10.1371/journal.pone.0162822
PMID: 27622704
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0162822