دورية أكاديمية
Chondroprotective effect of high-molecular-weight hyaluronic acid on osteoarthritic chondrocytes in a co-cultivation inflammation model with M1 macrophages.
العنوان: | Chondroprotective effect of high-molecular-weight hyaluronic acid on osteoarthritic chondrocytes in a co-cultivation inflammation model with M1 macrophages. |
---|---|
المؤلفون: | Bauer C; Center for Regenerative Medicine and Orthopedics, Department for Health Sciences and Biomedicine, Danube-University Krems, Dr.-Karl-Dorrek-Strasse 30, Krems, Austria., Niculescu-Morzsa E; Center for Regenerative Medicine and Orthopedics, Department for Health Sciences and Biomedicine, Danube-University Krems, Dr.-Karl-Dorrek-Strasse 30, Krems, Austria., Jeyakumar V; Center for Regenerative Medicine and Orthopedics, Department for Health Sciences and Biomedicine, Danube-University Krems, Dr.-Karl-Dorrek-Strasse 30, Krems, Austria., Kern D; Center for Regenerative Medicine and Orthopedics, Department for Health Sciences and Biomedicine, Danube-University Krems, Dr.-Karl-Dorrek-Strasse 30, Krems, Austria., Späth SS; Center for Regenerative Medicine and Orthopedics, Department for Health Sciences and Biomedicine, Danube-University Krems, Dr.-Karl-Dorrek-Strasse 30, Krems, Austria., Nehrer S; Center for Regenerative Medicine and Orthopedics, Department for Health Sciences and Biomedicine, Danube-University Krems, Dr.-Karl-Dorrek-Strasse 30, Krems, Austria. |
المصدر: | Journal of inflammation (London, England) [J Inflamm (Lond)] 2016 Sep 13; Vol. 13 (1), pp. 31. Date of Electronic Publication: 2016 Sep 13 (Print Publication: 2016). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101232234 Publication Model: eCollection Cited Medium: Print ISSN: 1476-9255 (Print) Linking ISSN: 14769255 NLM ISO Abbreviation: J Inflamm (Lond) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [London] : BioMed Central, 2004- |
مستخلص: | Background: Osteoarthritis (OA) is described by an imbalance between anabolic and catabolic processes in the affected joint. This dysregulation of metabolism affects not only chondrocytes within cartilage tissue but also the cells of the synovial membrane across the border of the joint. An important factor in OA is the low viscosity of the synovial fluid. High-molecular-weight hyaluronic acid (HA) can be used to increase the viscosity and also reduce inflammatory processes. The purpose was to establish an in vitro inflammation model and to evaluate the effects of high-molecular-weight HA in a co-cultivation inflammation model of osteoarthritic chondrocytes and M1 macrophages. Methods: For the establishment of the inflammation model THP-1 cells were, at first, differentiated to M0 macrophages and then activated to the M1 subtype after 5 days of resting period. Surface markers, cytokine release, and gene expression, were analyzed to examine the successful differentiation. In the inflammation model, the defined M1 macrophages were co-cultivated with osteoarthritic chondrocytes for 2 days, with and without the addition of 10 % HA and further analyzed for chondrogenic gene expression markers and the release of cytokines in the supernatant. Results: The differentiation and activation process was successful as M1 macrophages expressed higher levels of pro-inflammatory cytokines and specific genes. Similarly, the surface marker CD14 was significantly decreased compared to M0 macrophages. For the co-culture system, the analysis of gene expression showed that HA increased the expression of cartilage-specific genes while catabolic-encoding genes exhibited lower expression levels than the control group. This positive effect of HA was also demonstrated by the measurement of pro-inflammatory cytokines, as their level decreased. Conclusion: Our study implies that high-molecular-weight HA has a chondroprotective effect in the present co-cultivation inflammation model, as it decreases pro-inflammatory cytokines and increases anabolic factors. |
References: | Mediators Inflamm. 2015;2015:816460. (PMID: 26089604) J Clin Invest. 2011 Mar;121(3):985-97. (PMID: 21317534) Osteoarthritis Cartilage. 2002 Apr;10(4):277-81. (PMID: 11950250) Rheumatol Int. 2011 Apr;31(4):427-44. (PMID: 21113807) J Vasc Surg. 2001 Dec;34(6):1111-8. (PMID: 11743569) Immunol Rev. 2012 Jul;248(1):228-39. (PMID: 22725965) Mediators Inflamm. 2016;2016:8727289. (PMID: 27212811) Arthritis Res Ther. 2014;16(5):429. (PMID: 25606593) Osteoarthritis Cartilage. 2002 Jan;10(1):62-70. (PMID: 11795984) Arthritis Res Ther. 2014 Jan 21;16(1):R18. (PMID: 24443804) Front Immunol. 2014 Oct 07;5:491. (PMID: 25339958) Br J Oral Maxillofac Surg. 1998 Feb;36(1):35-8. (PMID: 9578254) World J Clin Cases. 2015 Feb 16;3(2):89-101. (PMID: 25685755) Nat Rev Rheumatol. 2015 Jan;11(1):21-34. (PMID: 25247412) Metallomics. 2009 Nov;1(6):471-8. (PMID: 21305155) Arthritis Res Ther. 2006;8(6):R187. (PMID: 17177994) Curr Rheumatol Rep. 2013 Nov;15(11):375. (PMID: 24072604) Inflamm Res. 2007 Jan;56(1):45-50. (PMID: 17334670) Curr Opin Rheumatol. 2011 Sep;23(5):471-8. (PMID: 21788902) J Biol Chem. 2012 Mar 9;287(11):8468-83. (PMID: 22270361) Toxicol Lett. 2010 Jun 2;195(2-3):99-105. (PMID: 20226236) Am J Reprod Immunol. 2013 Jul;70(1):80-6. (PMID: 23621670) Am J Sports Med. 2014 Jan;42(1):35-41. (PMID: 24192391) Arthritis Rheum. 1988 Jun;31(6):784-8. (PMID: 3260102) Cell Death Dis. 2014 May 15;5:e1228. (PMID: 24832600) Osteoarthritis Cartilage. 2014 Jan;22(1):111-20. (PMID: 24185105) J Leukoc Biol. 1996 Apr;59(4):555-61. (PMID: 8613704) Nephrol Dial Transplant. 2015 Aug;30 Suppl 4:iv60-7. (PMID: 26209740) Stem Cell Res Ther. 2013;4 Suppl 1:S5. (PMID: 24564965) Clin Cases Miner Bone Metab. 2015 Jan-Apr;12(1):31-3. (PMID: 26136793) Inflamm Res. 2014 Aug;63(8):691-701. (PMID: 24858301) PLoS One. 2010 Jan 13;5(1):e8668. (PMID: 20084270) Curr Rheumatol Rep. 2013 May;15(5):323. (PMID: 23516014) Carbohydr Polym. 2012 Jul 1;89(3):723-39. (PMID: 24750856) Mol Cell Endocrinol. 2011 Mar 15;335(1):2-13. (PMID: 20398732) J Leukoc Biol. 1991 Jun;49(6):556-65. (PMID: 1709200) Osteoarthritis Cartilage. 2014 Jan;22(1):121-7. (PMID: 24185110) Osteoarthritis Cartilage. 2012 Oct;20(10):1170-8. (PMID: 22796508) Phys Ther Sport. 2013 May;14(2):116-23. (PMID: 23506791) Open Orthop J. 2013 Sep 20;7:378-84. (PMID: 24093052) Methods. 2001 Dec;25(4):402-8. (PMID: 11846609) Adv Drug Deliv Rev. 2016 Feb 1;97:186-203. (PMID: 26541745) Arthritis Res Ther. 2003;5(2):54-67. (PMID: 12718745) Nat Rev Rheumatol. 2010 Nov;6(11):625-35. (PMID: 20924410) Swiss Med Wkly. 2010 Sep 17;140:w13098. (PMID: 20458653) BMC Musculoskelet Disord. 2015 Oct 26;16:321. (PMID: 26503103) Int J Rheum Dis. 2012 Feb;15(1):36-44. (PMID: 22324945) Nat Rev Rheumatol. 2011 Jan;7(1):33-42. (PMID: 21119608) J Skin Cancer. 2012;2012:393681. (PMID: 22792469) |
فهرسة مساهمة: | Keywords: Chondrocytes; Hyaluronic acid; Inflammation; Macrophages |
تواريخ الأحداث: | Date Created: 20160915 Date Completed: 20160914 Latest Revision: 20231111 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC5020517 |
DOI: | 10.1186/s12950-016-0139-y |
PMID: | 27625590 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1476-9255 |
---|---|
DOI: | 10.1186/s12950-016-0139-y |