دورية أكاديمية
أعرض في EDS

Multi-tiered Reorganization of the Genome during B Cell Affinity Maturation Anchored by a Germinal Center-Specific Locus Control Region.

التفاصيل البيبلوغرافية
العنوان: Multi-tiered Reorganization of the Genome during B Cell Affinity Maturation Anchored by a Germinal Center-Specific Locus Control Region.
المؤلفون: Bunting KL; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA; Department of Pharmacology, Weill Cornell Medical College, New York, NY 10065, USA., Soong TD; Institute for Computational Biomedicine, Weill Cornell Medical College, New York, NY 10065, USA., Singh R; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA; Department of Immunology and Microbial Pathogenesis, Weill Cornell Medical College, New York, NY 10065, USA., Jiang Y; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA; Institute for Computational Biomedicine, Weill Cornell Medical College, New York, NY 10065, USA; Department of Physiology and Biophysics, Weill Cornell Medical College, New York, NY 10065, USA., Béguelin W; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA., Poloway DW; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA., Swed BL; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA., Hatzi K; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA., Reisacher W; Department of Otorhinolaryngology, Weill Cornell Medical College/New York Presbyterian Hospital, New York, NY 10065, USA., Teater M; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA; Institute for Computational Biomedicine, Weill Cornell Medical College, New York, NY 10065, USA., Elemento O; Institute for Computational Biomedicine, Weill Cornell Medical College, New York, NY 10065, USA. Electronic address: ole2001@med.cornell.edu., Melnick AM; Division of Hematology and Medical Oncology, Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA; Department of Pharmacology, Weill Cornell Medical College, New York, NY 10065, USA. Electronic address: amm2014@med.cornell.edu.
المصدر: Immunity [Immunity] 2016 Sep 20; Vol. 45 (3), pp. 497-512. Date of Electronic Publication: 2016 Sep 13.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 9432918 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4180 (Electronic) Linking ISSN: 10747613 NLM ISO Abbreviation: Immunity Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, MA : Cell Press
Original Publication: Cambridge, Mass. : Cell Press, c1994-
مواضيع طبية MeSH: Antibody Affinity/*immunology , B-Lymphocytes/*immunology , Genome/*immunology , Germinal Center/*immunology , Locus Control Region/*immunology, Animals ; Antibody Formation/immunology ; Chromosomes, Human, Pair 3/immunology ; Epigenesis, Genetic/immunology ; Humans ; Immunity, Humoral/immunology ; Mice ; Promoter Regions, Genetic/immunology ; Proto-Oncogene Proteins c-bcl-6/immunology
مستخلص: During the humoral immune response, B cells undergo a dramatic change in phenotype to enable antibody affinity maturation in germinal centers (GCs). Using genome-wide chromosomal conformation capture (Hi-C), we found that GC B cells undergo massive reorganization of the genomic architecture that encodes the GC B cell transcriptome. Coordinate expression of genes that specify the GC B cell phenotype-most prominently BCL6-was achieved through a multilayered chromatin reorganization process involving (1) increased promoter connectivity, (2) formation of enhancer networks, (3) 5' to 3' gene looping, and (4) merging of gene neighborhoods that share active epigenetic marks. BCL6 was an anchor point for the formation of GC-specific gene and enhancer loops on chromosome 3. Deletion of a GC-specific, highly interactive locus control region upstream of Bcl6 abrogated GC formation in mice. Thus, large-scale and multi-tiered genomic three-dimensional reorganization is required for coordinate expression of phenotype-driving gene sets that determine the unique characteristics of GC B cells.
(Copyright © 2016 Elsevier Inc. All rights reserved.)
التعليقات: Comment in: Immunity. 2016 Sep 20;45(3):459-461. doi: 10.1016/j.immuni.2016.08.020. (PMID: 27653595)
Comment in: Nat Rev Immunol. 2016 Sep 27;16(10):594-5. doi: 10.1038/nri.2016.108. (PMID: 27670367)
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معلومات مُعتمدة: P30 DK020541 United States DK NIDDK NIH HHS; R01 CA104348 United States CA NCI NIH HHS; R01 CA187109 United States CA NCI NIH HHS; R01 CA194547 United States CA NCI NIH HHS
المشرفين على المادة: 0 (Proto-Oncogene Proteins c-bcl-6)
تواريخ الأحداث: Date Created: 20160918 Date Completed: 20170905 Latest Revision: 20240610
رمز التحديث: 20240610
مُعرف محوري في PubMed: PMC5033726
DOI: 10.1016/j.immuni.2016.08.012
PMID: 27637145
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-4180
DOI:10.1016/j.immuni.2016.08.012