| المؤلفون: |
Roma PM; Universidade Federal de Minas Gerais, Departamento de Física, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil., Siman L; Universidade Federal de Minas Gerais, Departamento de Física, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil., Hissa B; University of Chicago, James Franck Institute, Institute for Biophysical Dynamics and Physics Department, Chicago, Illinois 60637, United States., Agero U; Universidade Federal de Minas Gerais, Departamento de Física, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil., Braga EM; Universidade Federal de Minas Gerais, Departamento de Parasitologia, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil., Mesquita ON; Universidade Federal de Minas Gerais, Departamento de Física, Avenida Antônio Carlos 6627, Belo Horizonte, Minas Gerais, Brazil. |
| مستخلص: |
We use a quantitative phase imaging technique, defocusing microscopy (DM), to measure morphological, chemical, and mechanical parameters of individual red blood cells (RBCs) immersed in solutions with different osmolalities. We monitor the RBCs’ radius, volume, surface area, sphericity index, and hemoglobin content and concentration. The complete shape of cells is recovered and the effects of their adhesion to the glass substrate are observed. Finally, membrane fluctuation measurements give us information about the cells deformability. |
