دورية أكاديمية
Detection of Helicobacter pylori resistance to clarithromycin and fluoroquinolones in Brazil: A national survey.
| العنوان: | Detection of Helicobacter pylori resistance to clarithromycin and fluoroquinolones in Brazil: A national survey. |
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| المؤلفون: | Sanches BS; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Martins GM; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Lima K; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Cota B; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Moretzsohn LD; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Ribeiro LT; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Breyer HP; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Maguilnik I; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Maia AB; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Rezende-Filho J; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Meira AC; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Pinto H; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Alves E; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Mascarenhas R; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Passos R; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., de Souza JD; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Trindade OR; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil., Coelho LG; Bruno Squarcio Sanches, Gustavo Miranda Martins, Karine Lima, Bianca Cota, Luciana Dias Moretzsohn, Ana Carolina Meira, Henrique Pinto, Edson Alves, Raissa Passos, Julia Duarte de Souza, Osmar Reni Trindade, Luiz Gonzaga Coelho, Instituto Alfa de Gastroenterologia, Hospital das Clínicas, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais 30130-100, Brazil. |
| المصدر: | World journal of gastroenterology [World J Gastroenterol] 2016 Sep 07; Vol. 22 (33), pp. 7587-94. |
| نوع المنشور: | Journal Article; Multicenter Study; Observational Study |
| اللغة: | English |
| بيانات الدورية: | Publisher: Baishideng Publishing Group Country of Publication: United States NLM ID: 100883448 Publication Model: Print Cited Medium: Internet ISSN: 2219-2840 (Electronic) Linking ISSN: 10079327 NLM ISO Abbreviation: World J Gastroenterol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2014- : Pleasanton, CA : Baishideng Publishing Group Original Publication: Beijing : WJG Press, c1998- |
| مواضيع طبية MeSH: | Drug Resistance, Bacterial*, Anti-Bacterial Agents/*pharmacology , Clarithromycin/*pharmacology , Fluoroquinolones/*pharmacology , Helicobacter pylori/*isolation & purification, Adolescent ; Adult ; Aged ; Biopsy ; Brazil ; Endoscopy ; Female ; Helicobacter Infections/microbiology ; Helicobacter pylori/genetics ; Humans ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Mutation ; Stomach/pathology ; Young Adult |
| مستخلص: | Aim: To evaluate bacterial resistance to clarithromycin and fluoroquinolones in Brazil using molecular methods. Methods: The primary antibiotic resistance rates of Helicobacter pylori (H. pylori) were determined from November 2012 to March 2015 in the Southern, South-Eastern, Northern, North-Eastern, and Central-Western regions of Brazil. Four hundred ninety H. pylori patients [66% female, mean age 43 years (range: 18-79)] who had never been previously treated for this infection were enrolled. All patients underwent gastroscopy with antrum and corpus biopsies and molecular testing using GenoType HelicoDR (Hain Life Science, Germany). This test was performed to detect the presence of H. pylori and to identify point mutations in the genes responsible for clarithromycin and fluoroquinolone resistance. The molecular procedure was divided into three steps: DNA extraction from the biopsies, multiplex amplification, and reverse hybridization. Results: Clarithromycin resistance was found in 83 (16.9%) patients, and fluoroquinolone resistance was found in 66 (13.5%) patients. There was no statistical difference in resistance to either clarithromycin or fluoroquinolones (P = 0.55 and P = 0.06, respectively) among the different regions of Brazil. Dual resistance to clarithromycin and fluoroquinolones was found in 4.3% (21/490) of patients. The A2147G mutation was present in 90.4% (75/83), A2146G in 16.9% (14/83) and A2146C in 3.6% (3/83) of clarithromycin-resistant patients. In 10.8% (9/83) of clarithromycin-resistant samples, more than 01 mutation in the 23S rRNA gene was noticed. In fluoroquinolone-resistant samples, 37.9% (25/66) showed mutations not specified by the GenoType HelicoDR test. D91N mutation was observed in 34.8% (23/66), D91G in 18.1% (12/66), N87K in 16.6% (11/66) and D91Y in 13.6% (9/66) of cases. Among fluoroquinolone-resistant samples, 37.9% (25/66) showed mutations not specified by the GenoType HelicoDR test. Conclusion: The H. pylori clarithromycin resistance rate in Brazil is at the borderline (15%-20%) for applying the standard triple therapy. The fluoroquinolone resistance rate (13.5%) is equally concerning. Competing Interests: Conflict-of-interest statement: The authors have no conflict of interest to declare. |
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| فهرسة مساهمة: | Keywords: Clarithromycin; Fluoroquinolones; Helicobacter pylori; Microbial drug resistance; Molecular diagnostic techniques |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (Fluoroquinolones) H1250JIK0A (Clarithromycin) |
| تواريخ الأحداث: | Date Created: 20160928 Date Completed: 20170519 Latest Revision: 20201209 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC5011672 |
| DOI: | 10.3748/wjg.v22.i33.7587 |
| PMID: | 27672279 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2219-2840 |
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| DOI: | 10.3748/wjg.v22.i33.7587 |