دورية أكاديمية
Postictal alterations induced by intrahippocampal injection of pilocarpine in C57BL/6 mice.
| العنوان: | Postictal alterations induced by intrahippocampal injection of pilocarpine in C57BL/6 mice. |
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| المؤلفون: | Lima IVA; Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil., Campos AC; Department of Pharmacology, Universidade de São Paulo, Ribeirão Preto 14049-900, Brazil., Bellozi PMQ; Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil., Doria JG; Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil., Ribeiro FM; Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil., Moraes MFD; Department of Biophysics and Physiology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil. Electronic address: mfdm@icb.ufmg.br., de Oliveira ACP; Department of Pharmacology, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, Brazil. Electronic address: antoniooliveira@icb.ufmg.br. |
| المصدر: | Epilepsy & behavior : E&B [Epilepsy Behav] 2016 Nov; Vol. 64 (Pt A), pp. 83-89. Date of Electronic Publication: 2016 Oct 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 100892858 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-5069 (Electronic) Linking ISSN: 15255050 NLM ISO Abbreviation: Epilepsy Behav Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: San Diego, CA : Academic Press, c2000- |
| مواضيع طبية MeSH: | Cell Death/*drug effects , Epilepsy, Temporal Lobe/*chemically induced , Gliosis/*chemically induced , Hippocampus/*drug effects , Memory Disorders/*chemically induced , Muscarinic Agonists/*pharmacology , Neurons/*drug effects , Pilocarpine/*pharmacology , Status Epilepticus/*chemically induced, Animals ; Disease Models, Animal ; Male ; Mice ; Mice, Inbred C57BL ; Muscarinic Agonists/administration & dosage ; Pilocarpine/administration & dosage |
| مستخلص: | Temporal lobe epilepsy (TLE) is the most common form of epilepsy in adults. The pilocarpine (PILO) experimental model of TLE portrays behavioral and pathophysiological changes in rodents that are very similar to those found in humans with TLE. However, this model is associated with an unfortunate high mortality rate. Studies have shown that intrahippocampal injection of PILO, while having a much smaller mortality rate, induces status epilepticus (SE) that secondarily leads to TLE. To the best of our knowledge, the present study was the first to evaluate the cognitive and histological alterations 72h after intrahippocampal microinjection of PILO in C57BL/6 mice. Seventy percent of mice developed status epilepticus (SE) after PILO administration, and all animals survived after SE. Seventy-two hours after SE, mice presented memory impairment in both Novel Object Recognition (recognition index - vehicle: 67.57±4.46% vs PILO: 52.33±3.29%) and Contextual Fear Conditioning (freezing time - vehicle: 203±20.43 vs PILO: 107.80±25.17s) tasks. Moreover, using Nissl and NeuN staining, we observed in PILO-treated mice a significant decrease in cell viability and an increase in neuronal loss in all three hippocampal regions analyzed, cornus ammonis (CA) 1, CA3, and dentate gyrus (DG), in comparison with the control group. Additionally, using Iba-1 staining, we observed in PILO-treated mice a significant increase in microglial proliferation in CA1, CA3, and DG of the hippocampus. Therefore, intrahippocampal PILO microinjection is an efficient route to induce SE and acute postictal epileptogenic-like alterations in C57BL/6 mice. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Memory; Microglia; Neurodegeneration; Pilocarpine; Temporal lobe epilepsy |
| المشرفين على المادة: | 0 (Muscarinic Agonists) 01MI4Q9DI3 (Pilocarpine) |
| تواريخ الأحداث: | Date Created: 20161014 Date Completed: 20170904 Latest Revision: 20220330 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.yebeh.2016.08.003 |
| PMID: | 27736661 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1525-5069 |
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| DOI: | 10.1016/j.yebeh.2016.08.003 |