دورية أكاديمية
أعرض في EDS

Loss of ncm 5 and mcm 5 wobble uridine side chains results in an altered metabolic profile.

التفاصيل البيبلوغرافية
العنوان: Loss of ncm 5 and mcm 5 wobble uridine side chains results in an altered metabolic profile.
المؤلفون: Karlsborn T; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden., Mahmud AKMF; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden., Tükenmez H; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden., Byström AS; Department of Molecular Biology, Umeå University, 901 87 Umeå, Sweden.
المصدر: Metabolomics : Official journal of the Metabolomic Society [Metabolomics] 2016; Vol. 12 (12), pp. 177. Date of Electronic Publication: 2016 Sep 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 101274889 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1573-3882 (Print) Linking ISSN: 15733882 NLM ISO Abbreviation: Metabolomics Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: New York : Springer, c2006-
مستخلص: Introduction: The Elongator complex, comprising six subunits (Elp1p-Elp6p), is required for formation of 5-carbamoylmethyl (ncm 5 ) and 5-methoxycarbonylmethyl (mcm 5 ) side chains on wobble uridines in 11 out of 42 tRNA species in Saccharomyces cerevisiae . Loss of these side chains reduces the efficiency of tRNA decoding during translation, resulting in pleiotropic phenotypes. Overexpression of hypomodified [Formula: see text], which in wild-type strains are modified with mcm 5 s 2 U, partially suppress phenotypes of an elp3Δ strain.
Objectives: To identify metabolic alterations in an elp3Δ strain and elucidate whether these metabolic alterations are suppressed by overexpression of hypomodified [Formula: see text].
Method: Metabolic profiles were obtained using untargeted GC-TOF-MS of a temperature-sensitive elp3Δ strain carrying either an empty low-copy vector, an empty high-copy vector, a low-copy vector harboring the wild-type ELP3 gene, or a high-copy vector overexpressing [Formula: see text]. The temperature sensitive elp3Δ strain derivatives were cultivated at permissive (30 °C) or semi-permissive (34 °C) growth conditions.
Results: Culturing an elp3Δ strain at 30 or 34 °C resulted in altered metabolism of 36 and 46 %, respectively, of all metabolites detected when compared to an elp3Δ strain carrying the wild-type ELP3 gene. Overexpression of hypomodified [Formula: see text] suppressed a subset of the metabolic alterations observed in the elp3Δ strain.
Conclusion: Our results suggest that the presence of ncm 5 - and mcm 5 -side chains on wobble uridines in tRNA are important for metabolic homeostasis.
Competing Interests: Compliance with ethical standardsConflict of interestAuthor Anders S. Byström declares that he has no conflict of interest. Author Tony Karlsborn declares that he has no conflict of interest. Author A K M Firoj Mahmud declares that he has no conflict of interest. Author Hasan Tükenmez declares that he has no conflict of interest.Ethical approvalThis article does not contain any studies with human participants or animals performed by any of the authors.
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فهرسة مساهمة: Keywords: ELP3; Elongator complex; Metabolic profiling; Metabolomics; Translation; tRNA wobble uridine modifications
تواريخ الأحداث: Date Created: 20161015 Latest Revision: 20191120
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC5037161
DOI: 10.1007/s11306-016-1120-8
PMID: 27738410
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-3882
DOI:10.1007/s11306-016-1120-8