دورية أكاديمية
Novel cellular evidence of lipophagy within the Sertoli cells during spermatogenesis in the turtle.
| العنوان: | Novel cellular evidence of lipophagy within the Sertoli cells during spermatogenesis in the turtle. |
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| المؤلفون: | Ahmed N; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China.; Faculty of Veterinary and Animal Sciences, LUAWMS, Uthal 90150, Pakistan., Liu Y; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Chen H; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Yang P; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Waqas Y; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Liu T; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Gandahi JA; Faculty of Animal Husbandry and Veterinary Sciences, Sindh Agriculture University, Tando Jam 70060, Pakistan., Huang Y; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Wang L; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Song X; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Rajput IR; Faculty of Veterinary and Animal Sciences, LUAWMS, Uthal 90150, Pakistan., Wang T; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China., Chen Q; Laboratory of Animal Cell Biology and Embryology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China. |
| المصدر: | Aging [Aging (Albany NY)] 2016 Oct 16; Vol. 9 (1), pp. 41-51. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Impact Journals, LLC Country of Publication: United States NLM ID: 101508617 Publication Model: Print Cited Medium: Internet ISSN: 1945-4589 (Electronic) Linking ISSN: 19454589 NLM ISO Abbreviation: Aging (Albany NY) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Albany, NY : Impact Journals, LLC |
| مواضيع طبية MeSH: | Autophagy/*physiology , Lipid Droplets/*metabolism , Lipid Metabolism/*physiology , Sertoli Cells/*metabolism , Spermatogenesis/*physiology , Turtles/*physiology, Animals ; Male ; Seminiferous Tubules/metabolism ; Spermatozoa/metabolism |
| مستخلص: | Spermatogenesis is a complex process producing haploid spermatozoa, and the formation of lipid droplets (LDs) within Sertoli cells is critical to maintaining normal spermatogenesis. However, the utilization of LDs within Sertoli cells is still largely unknown. In the present study, proliferation of spermatogonial cells had begun in May, whereas the meiotic cells occurred predominately in July and majority of spermiogenic cells were observed in the seminiferous tubules in October. However, TEM and Oil Red O staining demonstrated that a larger number of LDs had accumulated within the Sertoli cells in May compared to that in October. There were several LDs attached to the isolation membrane/phagophore, suggesting that the LDs may be a source of endogenous energy for the biogenesis of autophagosomes. The LDs were enclosed within the autophagosomes in May, whereas, autophagosomes and mitochondria were directly attached with large LDs within the Sertoli cells in October. Furthermore, immunohistochemistry results demonstrated the stronger localization of LC3 on the Sertoli cells in May than in October. This study is the first to provide clear evidence of the two different modes of lipophagy for lipid consumption within Sertoli cells, which is a key aspect of Sertoli germ cell communication during spermatogenesis. |
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| فهرسة مساهمة: | Keywords: Chinese soft-shelled turtle; LC3; Sertoli cell; lipophagy; spermatogenesis |
| تواريخ الأحداث: | Date Created: 20161018 Date Completed: 20171116 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5310655 |
| DOI: | 10.18632/aging.101070 |
| PMID: | 27750210 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1945-4589 |
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| DOI: | 10.18632/aging.101070 |