دورية أكاديمية
Next-Generation Sequencing Analysis of Long Noncoding RNAs in CD4+ T Cell Differentiation.
| العنوان: | Next-Generation Sequencing Analysis of Long Noncoding RNAs in CD4+ T Cell Differentiation. |
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| المؤلفون: | Ranzani V; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy., Arrigoni A; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy., Rossetti G; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy., Panzeri I; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy., Abrignani S; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy.; Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Via Festa del Perdono 7, Milan, 20122, Italy., Bonnal RJ; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy. bonnal@ingm.org., Pagani M; Istituto Nazionale Genetica Molecolare 'Romeo ed Enrica Invernizzi', Via F. Sforza 35, Milan, 20122, Italy. pagani@ingm.org.; Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, Via Festa del Perdono 7, Milan, 20122, Italy. pagani@ingm.org. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1514, pp. 173-185. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Cell Differentiation/*genetics , Gene Expression Profiling/*methods , High-Throughput Nucleotide Sequencing/*methods , RNA, Long Noncoding/*immunology, CD4-Positive T-Lymphocytes/immunology ; CD8-Positive T-Lymphocytes/immunology ; Genome ; Humans ; RNA, Long Noncoding/genetics |
| مستخلص: | Next-generation sequencing approaches, in particular RNA-seq, provide a genome-wide expression profiling allowing the identification of novel and rare transcripts such as long noncoding RNAs (lncRNA). Many RNA-seq studies have now been performed aimed at the characterization of lncRNAs and their possible involvement in cell development and differentiation in different organisms, cell types, and tissues. The adaptive immune system is an extraordinary context for the study of the role of lncRNAs in differentiation. Indeed lncRNAs seem to be key drivers in governing flexibility and plasticity of both CD8 + and CD4 + T cell, together with lineage-specific transcription factors and cytokines, acting as fine-tuners of fate choices in T cell differentiation.We describe here a pipeline for the identification of lncRNAs starting from RNA-Seq raw data. |
| فهرسة مساهمة: | Keywords: Bioinformatics; CD4+ T cells; Immune system; NGS; RNA-seq; lncRNAs |
| المشرفين على المادة: | 0 (RNA, Long Noncoding) |
| تواريخ الأحداث: | Date Created: 20161028 Date Completed: 20180110 Latest Revision: 20180416 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/978-1-4939-6548-9_14 |
| PMID: | 27787801 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-6548-9_14 |