دورية أكاديمية
The Influence of Sporulation Conditions on the Spore Coat Protein Composition of Bacillus subtilis Spores.
| العنوان: | The Influence of Sporulation Conditions on the Spore Coat Protein Composition of Bacillus subtilis Spores. |
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| المؤلفون: | Abhyankar WR; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of AmsterdamAmsterdam, Netherlands; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of AmsterdamAmsterdam, Netherlands., Kamphorst K; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam Amsterdam, Netherlands., Swarge BN; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of AmsterdamAmsterdam, Netherlands; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of AmsterdamAmsterdam, Netherlands., van Veen H; Department of Cell Biology and Histology, Electron Microscopy Centre Amsterdam, Academic Medical Center Amsterdam, Netherlands., van der Wel NN; Department of Cell Biology and Histology, Electron Microscopy Centre Amsterdam, Academic Medical Center Amsterdam, Netherlands., Brul S; Department of Molecular Biology and Microbial Food Safety, Swammerdam Institute for Life Sciences, University of Amsterdam Amsterdam, Netherlands., de Koster CG; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam Amsterdam, Netherlands., de Koning LJ; Department of Mass Spectrometry of Bio-macromolecules, Swammerdam Institute for Life Sciences, University of Amsterdam Amsterdam, Netherlands. |
| المصدر: | Frontiers in microbiology [Front Microbiol] 2016 Oct 13; Vol. 7, pp. 1636. Date of Electronic Publication: 2016 Oct 13 (Print Publication: 2016). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
| مستخلص: | Spores are of high interest to the food and health sectors because of their extreme resistance to harsh conditions, especially against heat. Earlier research has shown that spores prepared on solid agar plates have a higher heat resistance than those prepared under a liquid medium condition. It has also been shown that the more mature a spore is, the higher is its heat resistance most likely mediated, at least in part, by the progressive cross-linking of coat proteins. The current study for the first time assesses, at the proteomic level, the effect of two commonly used sporulation conditions on spore protein presence. 14 N spores prepared on solid Schaeffer's-glucose (SG) agar plates and 15 N metabolically labeled spores prepared in shake flasks containing 3-( N -morpholino) propane sulfonic acid (MOPS) buffered defined liquid medium differ in their coat protein composition as revealed by LC-FT-MS/MS analyses. The former condition mimics the industrial settings while the latter conditions mimic the routine laboratory environment wherein spores are developed. As seen previously in many studies, the spores prepared on the solid agar plates show a higher thermal resistance than the spores prepared under liquid culture conditions. The 14 N: 15 N isotopic ratio of the 1:1 mixture of the spore suspensions exposes that most of the identified inner coat and crust proteins are significantly more abundant while most of the outer coat proteins are significantly less abundant for the spores prepared on solid SG agar plates relative to the spores prepared in the liquid MOPS buffered defined medium. Sporulation condition-specific differences and variation in isotopic ratios between the tryptic peptides of expected cross-linked proteins suggest that the coat protein cross-linking may also be condition specific. Since the core dipicolinic acid content is found to be similar in both the spore populations, it appears that the difference in wet heat resistance is connected to the differences in the coat protein composition and assembly. Corroborating the proteomic analyses, electron microscopy analyses show a significantly thinner outer coat layer of the spores cultured on the solid agar medium. |
| References: | Biotechnol Bioeng. 2014 Apr;111(4):654-63. (PMID: 24203291) J Bacteriol. 2001 Sep;183(18):5426-30. (PMID: 11514528) J Bacteriol. 2010 Jan;192(2):518-24. (PMID: 19933362) Annu Rev Microbiol. 1988;42:319-38. (PMID: 3059997) Mol Microbiol. 2006 Jan;59(2):487-502. (PMID: 16390444) Food Microbiol. 2015 Feb;45(Pt A):54-62. (PMID: 25481062) J Appl Bacteriol. 1983 Aug;55(1):39-48. (PMID: 6413477) Trends Microbiol. 2002 Jun;10(6):251-4. (PMID: 12088650) J Bacteriol. 2004 Sep;186(17):5567-75. (PMID: 15317760) Microbiol Spectr. 2016 Apr;4(2):null. (PMID: 27227299) Nat Rev Microbiol. 2013 Jan;11(1):33-44. (PMID: 23202530) J Bacteriol. 2011 Aug;193(16):4075-80. (PMID: 21665972) Proteomics. 2011 Dec;11(23):4541-50. (PMID: 21905219) Microbiol Mol Biol Rev. 1999 Mar;63(1):1-20. (PMID: 10066829) J Bacteriol. 2006 Jun;188(11):3740-7. (PMID: 16707666) Science. 1958 Jan 3;127(3288):26-7. (PMID: 13495474) J Bacteriol. 2004 Jul;186(14):4441-8. (PMID: 15231775) Anal Biochem. 1974 May;59(1):277-82. (PMID: 4407487) Nat Biotechnol. 2014 Mar;32(3):223-6. (PMID: 24727771) Annu Rev Food Sci Technol. 2015;6:351-69. (PMID: 25705935) Appl Environ Microbiol. 2011 Oct;77(19):6746-54. (PMID: 21821751) Curr Biol. 2010 May 25;20(10):934-8. (PMID: 20451384) Appl Environ Microbiol. 2008 Jun;74(11):3328-35. (PMID: 18378644) J Appl Microbiol. 2002;92(6):1105-15. (PMID: 12010551) Mol Microbiol. 2001 Nov;42(4):1107-20. (PMID: 11737650) J Appl Microbiol. 2007 Sep;103(3):691-9. (PMID: 17714403) |
| فهرسة مساهمة: | Keywords: Bacillus; proteomics; quantitative proteomics; spores; sporulation conditions |
| تواريخ الأحداث: | Date Created: 20161030 Latest Revision: 20201001 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5061772 |
| DOI: | 10.3389/fmicb.2016.01636 |
| PMID: | 27790212 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1664-302X |
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| DOI: | 10.3389/fmicb.2016.01636 |