دورية أكاديمية
Fungal Microbiota Profile in Newly Diagnosed Treatment-naïve Children with Crohn's Disease.
| العنوان: | Fungal Microbiota Profile in Newly Diagnosed Treatment-naïve Children with Crohn's Disease. |
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| المؤلفون: | El Mouzan M; Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia., Wang F; Bioinformatics Program, Boston University, Boston, MA, USA., Al Mofarreh M; Al Mofarreh Polyclinic, Department of Gastroenterology, Riyadh, Kingdom of Saudi Arabia., Menon R; Physics Department, Boston University, Boston, MA, USA., Al Barrag A; Department of Microbiology, King Saud University, Riyadh, Kingdom of Saudi Arabia., Korolev KS; Physics Department and Bioinformatics Program, Boston University, Boston, MA, USA., Al Sarkhy A; Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia., Al Asmi M; Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia., Hamed Y; Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia., Saeed A; Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia., Dowd SE; MRDNA Laboratories, Shallowater, TX, USA., Assiri A; Department of Pediatrics, Gastroenterology Division, King Saud University, Riyadh, Kingdom of Saudi Arabia., Winter H; Pediatric IBD Program, MassGeneral Hospital for Children, Boston, MA, USA. |
| المصدر: | Journal of Crohn's & colitis [J Crohns Colitis] 2017 May 01; Vol. 11 (5), pp. 586-592. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 101318676 Publication Model: Print Cited Medium: Internet ISSN: 1876-4479 (Electronic) Linking ISSN: 18739946 NLM ISO Abbreviation: J Crohns Colitis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Oxford : Oxford University Press Original Publication: Amsterdam : Elsevier Science |
| مواضيع طبية MeSH: | Crohn Disease*/diagnosis , Crohn Disease*/epidemiology , Crohn Disease*/microbiology , Fungi*/classification , Fungi*/genetics , Fungi*/isolation & purification , Intestinal Mucosa*/microbiology , Intestinal Mucosa*/pathology, Mycobiome/*physiology, Adolescent ; Child ; Colonoscopy/methods ; Dysbiosis/complications ; Dysbiosis/diagnosis ; Female ; Humans ; Male ; Phylogeny ; Prospective Studies ; Saudi Arabia/epidemiology ; Statistics as Topic |
| مستخلص: | Background and Aims: Although increasing evidence suggests a role for fungi in inflammatory bowel disease [IBD], data are scarce and mostly from adults. Our aim was to define the characteristics of fungal microbiota in newly diagnosed treatment-naïve children with Crohn's disease [CD]. Methods: The children referred for colonoscopy were prospectively enrolled in the study at King Khalid University Hospital, King Saud University, and Al Mofarreh Polyclinics in Riyadh. Tissue and stool samples were collected and frozen till sequencing analysis. The children with confirmed CD diagnosis were designated as cases and the others as non- IBD controls; 78 samples were collected from 35 children [15 CD and 20 controls]. Statistical analysis was performed to investigate CD associations and diversity. Results: CD-associated fungi varied with the level of phylogenetic tree. There was no significant difference in abundance between normal and inflamed mucosa. Significantly abundant CD-associated taxa included Psathyrellaceae [p = 0.01], Cortinariaceae [p = 0.04], Psathyrella [p = 0.003], and Gymnopilus [p = 0.03]. Monilinia was significantly depleted [p = 0.03], whereas other depleted taxa, although not statistically significant, included Leotiomycetes [p = 0.06], Helotiales [p = 0.08], and Sclerotiniaceae [p = 0.07]. There was no significant difference in fungal diversity between CD and controls. Conclusions: We report highly significant fungal dysbiosis in newly diagnosed treatment-naïve CD children. Depleted and more abundant taxa suggest anti-inflammatory and pro-inflamatory potentials, respectively. Further studies with larger sample size and including functional analysis are needed to clarify the significance of the fungal community in the pathogenesis of CD. (Copyright © 2017 European Crohn’s and Colitis Organisation (ECCO). Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com) |
| التعليقات: | Comment in: J Crohns Colitis. 2022 Jan 24;:. (PMID: 35073577) Erratum in: J Crohns Colitis. 2022 Aug 16;:. (PMID: 35971821) |
| فهرسة مساهمة: | Keywords: Crohn’s disease; Fungal dysbiosis; Saudi children. |
| تواريخ الأحداث: | Date Created: 20161105 Date Completed: 20180314 Latest Revision: 20220826 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1093/ecco-jcc/jjw197 |
| PMID: | 27811291 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1876-4479 |
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| DOI: | 10.1093/ecco-jcc/jjw197 |