دورية أكاديمية
Isolation, Culture, and Staining of Single Myofibers.
| العنوان: | Isolation, Culture, and Staining of Single Myofibers. |
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| المؤلفون: | Gallot YS; Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, USA., Hindi SM; Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, USA., Mann AK; duPont Manual High School, Louisville, USA., Kumar A; Department of Anatomical Sciences and Neurobiology, University of Louisville School of Medicine, Louisville, USA. |
| المصدر: | Bio-protocol [Bio Protoc] 2016 Oct 05; Vol. 6 (19). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bio-protocol LLC Country of Publication: United States NLM ID: 101635102 Publication Model: Print Cited Medium: Print ISSN: 2331-8325 (Print) Linking ISSN: 23318325 NLM ISO Abbreviation: Bio Protoc Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Sunnyvale, CA, USA : Bio-protocol LLC, [2011]- |
| مستخلص: | Adult skeletal muscle regeneration is orchestrated by a specialized population of adult stem cells called satellite cells, which are localized between the basal lamina and the plasma membrane of myofibers. The process of satellite cell-activation, proliferation, and subsequent differentiation that occurs during muscle regeneration can be recapitulated ex vivo by isolation of single myofibers from skeletal muscles and culturing them under suspension conditions. Here, we describe an improved protocol to evaluate ex vivo satellite cells activation through isolation of single myofibers from extensor digitorum longus (EDL) muscle of mice and culturing and staining of myofiber-associated satellite cells with the markers of self-renewal, proliferation, and differentiation. |
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| معلومات مُعتمدة: | F32 AR069985 United States AR NIAMS NIH HHS; R01 AG029623 United States AG NIA NIH HHS; R01 AR059810 United States AR NIAMS NIH HHS; R01 AR068313 United States AR NIAMS NIH HHS |
| تواريخ الأحداث: | Date Created: 20161108 Latest Revision: 20230313 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5094811 |
| DOI: | 10.21769/BioProtoc.1942 |
| PMID: | 27819014 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2331-8325 |
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| DOI: | 10.21769/BioProtoc.1942 |