دورية أكاديمية
A novel translational control mechanism involving RNA structures within coding sequences.
| العنوان: | A novel translational control mechanism involving RNA structures within coding sequences. |
|---|---|
| المؤلفون: | Jungfleisch J; Molecular Virology Group, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain., Nedialkova DD; Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.; Cells-in-Motion Cluster of Excellence, University of Münster, 48149 Münster, Germany., Dotu I; Research Programme on Biomedical Informatics (GRIB), Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Hospital del Mar Medical Research Institute (IMIM), 08003 Barcelona, Spain., Sloan KE; Institute for Molecular Biology, Göttingen University Medical Department, 37073 Göttingen, Germany., Martinez-Bosch N; Program of Cancer Research, Hospital del Mar Medical Research Institute (IMIM), 08003 Barcelona, Spain., Brüning L; Institute for Molecular Biology, Göttingen University Medical Department, 37073 Göttingen, Germany., Raineri E; Statistical Genomics, Centro Nacional de Analisis Genomica, 08028 Barcelona, Spain., Navarro P; Program of Cancer Research, Hospital del Mar Medical Research Institute (IMIM), 08003 Barcelona, Spain., Bohnsack MT; Institute for Molecular Biology, Göttingen University Medical Department, 37073 Göttingen, Germany.; Göttingen Center for Molecular Biosciences, Georg-August University, 37073 Göttingen, Germany., Leidel SA; Max Planck Research Group for RNA Biology, Max Planck Institute for Molecular Biomedicine, 48149 Münster, Germany.; Cells-in-Motion Cluster of Excellence, University of Münster, 48149 Münster, Germany.; Faculty of Medicine, University of Münster, 48149 Münster, Germany., Díez J; Molecular Virology Group, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, 08003 Barcelona, Spain. |
| المصدر: | Genome research [Genome Res] 2017 Jan; Vol. 27 (1), pp. 95-106. Date of Electronic Publication: 2016 Nov 07. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 9518021 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1549-5469 (Electronic) Linking ISSN: 10889051 NLM ISO Abbreviation: Genome Res Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1995- |
| مواضيع طبية MeSH: | Peptide Chain Initiation, Translational* , Protein Biosynthesis*, DEAD-box RNA Helicases/*genetics , RNA/*genetics , Saccharomyces cerevisiae Proteins/*genetics, Bromovirus/genetics ; Exons/genetics ; Gene Expression Regulation/genetics ; Humans ; Nucleic Acid Conformation ; Open Reading Frames/genetics ; RNA, Messenger/genetics ; Ribosomes/genetics ; Saccharomyces cerevisiae/genetics |
| مستخلص: | The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here, we identify a novel and highly conserved mechanism of translational control involving RNA structures within coding sequences and the DEAD-box helicase Dhh1. Using yeast genetics and genome-wide ribosome profiling analyses, we show that this mechanism, initially derived from studies of the Brome Mosaic virus RNA genome, extends to yeast and human mRNAs highly enriched in membrane and secreted proteins. All Dhh1-dependent mRNAs, viral and cellular, share key common features. First, they contain long and highly structured CDSs, including a region located around nucleotide 70 after the translation initiation site; second, they are directly bound by Dhh1 with a specific binding distribution; and third, complementary experimental approaches suggest that they are activated by Dhh1 at the translation initiation step. Our results show that ribosome translocation is not the only unwinding force of CDS and uncover a novel layer of translational control that involves RNA helicases and RNA folding within CDS providing novel opportunities for regulation of membrane and secretome proteins. (© 2017 Jungfleisch et al.; Published by Cold Spring Harbor Laboratory Press.) |
| التعليقات: | Erratum in: Genome Res. 2017 Apr;27(4):663. (PMID: 28373300) |
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| المشرفين على المادة: | 0 (RNA, Messenger) 0 (Saccharomyces cerevisiae Proteins) 63231-63-0 (RNA) EC 3.6.1.- (DHH1 protein, S cerevisiae) EC 3.6.4.13 (DEAD-box RNA Helicases) |
| تواريخ الأحداث: | Date Created: 20161109 Date Completed: 20180104 Latest Revision: 20201209 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC5204348 |
| DOI: | 10.1101/gr.209015.116 |
| PMID: | 27821408 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1549-5469 |
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| DOI: | 10.1101/gr.209015.116 |