دورية أكاديمية
Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice.
| العنوان: | Remote ischemic preconditioning and tacrolimus in the fetal small bowel transplant in mice. |
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| المؤلفون: | Morello RJ; PhD, Operative Technique and Experimental Surgery Division, Medical School, Universidade Federal de São Paulo (UNIFESP), Brazil. Technical procedures, acquisition of data, manuscript writing., Koike MK; PhD, Laboratory of Emergency Medicine - LIM-51, Faculty of Medicine (FM), Universidade de São Paulo (USP), Brazil. Interpretation of data, histopathological examinations, statistical analysis, manuscript writing., Abrahão MS; PhD, Operative Technique and Experimental Surgery Division, Medical School, Universidade Federal de São Paulo (UNIFESP), Brazil. Technical procedures, acquisition of data, manuscript writing., Saad KR; Associate Professor, Medical School, Universidade Federal do Vale do São Francisco (UNIVASF), Brazil. Manuscript writing, critical revision., Saad PF; Associate Professor, Medical School, Universidade Federal do Vale do São Francisco (UNIVASF), Brazil. Manuscript writing, critical revision., Montero EF; Associate Professor, Surgical Physiopathology Laboratory (LIM-62), FMUSP. Affiliate Professor, Operative Technique and Experimental Surgery Division, Medical School, UNIFESP, Sao Paulo-SP, Brazil. Conception, intellectual and scientific content of the study; critical revision; final approval. |
| المصدر: | Acta cirurgica brasileira [Acta Cir Bras] 2016 Oct; Vol. 31 (10), pp. 675-679. |
| نوع المنشور: | Evaluation Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Country of Publication: Brazil NLM ID: 9103983 Publication Model: Print Cited Medium: Internet ISSN: 1678-2674 (Electronic) Linking ISSN: 01028650 NLM ISO Abbreviation: Acta Cir Bras Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Sao Paulo : Sociedade Brasileira Para O Desenvolvimento Da Pesquisa Em Cirurgia Curso De Pos-Graduacao Em Tecnica Operatoria E Cirurgia Experimental Escola Paulista De Medicina Original Publication: São Paulo : A Sociedade, |
| مواضيع طبية MeSH: | Fetal Tissue Transplantation/*methods , Immunosuppressive Agents/*therapeutic use , Intestine, Small/*blood supply , Intestine, Small/*transplantation , Ischemic Preconditioning/*methods , Tacrolimus/*therapeutic use, Animals ; Cell Proliferation/drug effects ; Female ; Graft Rejection/prevention & control ; Immunohistochemistry ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Reproducibility of Results ; Time Factors ; Transplantation, Isogeneic ; Treatment Outcome |
| مستخلص: | Purpose:: To evaluate the effect of remote ischemic preconditioning (IPC-R) in the fetal small bowel transplantation model. Methods:: Two groups were constituted: The Isogenic transplant (ISO, C57BL/6 mice, n=24) and the allogenic transplant (ALO, BALB/c mice, n=24). In each group, the animals were distributed with and without IPC-R. It was obtained the following subgroups: Tx, IPC-R, Fk, IPC-Fk, in both strains. Intestinal grafts were stained with hematoxylin and eosin and immunohistochemically. Results:: The graft development evaluation in ISO group showed that IPC-R reduced the development compared with ISO-Tx (5.2±0.4 vs 9.0±0.8) and IPC-R-Fk increased the graft development compared with IPC-R (11.2±0.7 and 10.2±0.8). In ALO group, IPC-Fk increased the development compared with ALO-Tx and ALO with IPC-R (6.0±0.8, 9.0±1.2, 0.0±0.0, 0.5±0.3). The PCNA expression was increased in ISO group treated with Fk and IPC-R compared to other groups (12.2±0.8 vs Tx: 8.8±0.9, IPC-R: 8.0±0.4 and Fk: 9.0±0.6). The graft rejection was lower in groups treated with IPC-R (-18%), Fk (-68%) or both (-61%) compared with ALO-Tx. Conclusion:: Remote ischemic preconditioning showed benefic effect even associate with Tacrolimus on the development and acute rejection of the fetal small bowel graft in the Isogenic and Allogenic transplants. |
| المشرفين على المادة: | 0 (Immunosuppressive Agents) WM0HAQ4WNM (Tacrolimus) |
| تواريخ الأحداث: | Date Created: 20161110 Date Completed: 20170526 Latest Revision: 20191210 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1590/S0102-865020160100000006 |
| PMID: | 27828601 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1678-2674 |
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| DOI: | 10.1590/S0102-865020160100000006 |