دورية أكاديمية
In Vitro Exploration of the Anti-HCV Potential of the Synthetic Spacer Peptides Derived from Human, Bovine, and Camel Lactoferrins.
| العنوان: | In Vitro Exploration of the Anti-HCV Potential of the Synthetic Spacer Peptides Derived from Human, Bovine, and Camel Lactoferrins. |
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| المؤلفون: | Albar AH; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah. Saudi Arabia., El-Fakharany EM; Therapeutic and Protective Proteins Laboratory, Protein Research Department, Genetic Engineering and Biotechnology Research Institute, City for Scientific Research and Technology Applications, New Borg EL-Arab 21934, Alexandria. Egypt., Almehdar HA; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah. Saudi Arabia., Uversky VN; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah. Saudi Arabia., Redwan EM; Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah. Saudi Arabia. |
| المصدر: | Protein and peptide letters [Protein Pept Lett] 2017; Vol. 24 (10), pp. 909-921. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bentham Science Publishers Country of Publication: Netherlands NLM ID: 9441434 Publication Model: Print Cited Medium: Internet ISSN: 1875-5305 (Electronic) Linking ISSN: 09298665 NLM ISO Abbreviation: Protein Pept Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Hilversum, The Netherlands : Bentham Science Publishers Original Publication: Schiphol, The Netherlands : Bentham Science Publishers, c1994- |
| مواضيع طبية MeSH: | Antiviral Agents/*pharmacology , Hepacivirus/*drug effects , Lactoferrin/*pharmacology , Peptides/*pharmacology, Animals ; Camelus ; Cattle ; Cell Survival ; Hep G2 Cells ; Hepacivirus/physiology ; Humans ; Lactoferrin/chemistry ; Leukocytes, Mononuclear ; Peptides/chemistry ; Structure-Activity Relationship ; Virion/drug effects ; Virus Internalization/drug effects |
| مستخلص: | Background: Chronic liver disease is often associated with the infection by hepatitis C virus (HCV), which is an enveloped RNA virus belonging to the Flaviviridae family. Many studies found that milk proteins, such as lactoferrin, might have profound antiviral activity against HCV. Various secretory fluids ranging from milk, to tears, saliva, and nasal secretion, and to bile and pancreatic juice, as well as neutrophils, mucosal surfaces, and blood contain a widely spread multifunctional glycoprotein, lactoferrin (Lf), structure of which can be depicted as two homologous domains connected by the short spacer peptide. Objective: This study aimed to understand the effectiveness of the synthetic peptides cLfsp, bLfsp, hLfsp1, and hLfsp2 corresponding to the spacer peptides of camel, bovine, and human Lfs, respectively, against HCV in in vitro settings. Method: We used RT-nested PCR to evaluate the antiviral activity of the synthesized spacer peptides against HCV infectivity in PBMC and HepG2 cells looking at their neutralization, protection, and intracellular treatment potentials. Results and Conclusion: We show that direct interaction of hLfsp1, hLfsp2, and bLfsp with viral particles is able to neutralize the HCV entry into HepG2 cells (with hLfsp2 being more potent neutralizer than hLfsp1 and bLfsp), whereas cLfsp does not show any neutralizing potential. Therefore, our analysis revealed that different spacer peptides are characterized by different antiviral potentials and use different mechanisms for antiviral protection. (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.org.) |
| فهرسة مساهمة: | Keywords: Lactoferrin; antiviral activity; intracellular treatment potential; neutralization potential; protection potential; spacer peptide |
| المشرفين على المادة: | 0 (Antiviral Agents) 0 (Peptides) EC 3.4.21.- (Lactoferrin) |
| تواريخ الأحداث: | Date Created: 20161112 Date Completed: 20190115 Latest Revision: 20190115 |
| رمز التحديث: | 20240628 |
| DOI: | 10.2174/0929866524666161111111320 |
| PMID: | 27834141 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1875-5305 |
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| DOI: | 10.2174/0929866524666161111111320 |