دورية أكاديمية
أعرض في EDS

In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry.

التفاصيل البيبلوغرافية
العنوان: In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry.
المؤلفون: Lo Giudice MC; Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland., Herda LM; Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland., Polo E; Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland., Dawson KA; Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland.
المصدر: Nature communications [Nat Commun] 2016 Nov 15; Vol. 7, pp. 13475. Date of Electronic Publication: 2016 Nov 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : Nature Pub. Group
مواضيع طبية MeSH: Quantum Dots*, Flow Cytometry/*methods , Nanoparticles/*chemistry , Proteins/*chemistry, Algorithms ; Antibodies/chemistry ; Antibodies/metabolism ; Humans ; Nanoparticles/metabolism ; Protein Binding ; Proteins/immunology ; Proteins/metabolism ; Serum Albumin/chemistry ; Serum Albumin/immunology ; Serum Albumin/metabolism ; Spectrometry, Fluorescence ; Transferrin/chemistry ; Transferrin/immunology ; Transferrin/metabolism
مستخلص: Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented in complex biological milieu, which produce a bio-interface or 'biomolecular corona' conferring a biological identity to the particle. Biomolecules at the surface of the nanoparticle-biomolecule complex present molecular fragments that may be recognized by receptors of cells or biological barriers, potentially engaging with different biological pathways. Here we demonstrate that using intense fluorescent reporter binders, in this case antibodies bound to quantum dots, we can map out the availability of such recognition fragments, allowing for a rapid and meaningful biological characterization. The application in microfluidic flow, in small detection volumes, with appropriate thresholding of the detection allows the study of even complex nanoparticles in realistic biological milieu, with the emerging prospect of making direct connection to conditions of cell level and in vivo experiments.
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المشرفين على المادة: 0 (Antibodies)
0 (Proteins)
0 (Serum Albumin)
0 (Transferrin)
تواريخ الأحداث: Date Created: 20161116 Date Completed: 20180917 Latest Revision: 20240326
رمز التحديث: 20240326
مُعرف محوري في PubMed: PMC5116075
DOI: 10.1038/ncomms13475
PMID: 27845346
قاعدة البيانات: MEDLINE
الوصف
تدمد:2041-1723
DOI:10.1038/ncomms13475