دورية أكاديمية
A large fraction of HLA class I ligands are proteasome-generated spliced peptides.
| العنوان: | A large fraction of HLA class I ligands are proteasome-generated spliced peptides. |
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| المؤلفون: | Liepe J; Centre for Integrative Systems Biology and Bioinformatics, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK. michele.mishto@charite.de j.liepe@imperial.ac.uk., Marino F; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, Netherlands.; Netherlands Proteomics Centre, CH Utrecht, Netherlands., Sidney J; Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA., Jeko A; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, Netherlands.; Netherlands Proteomics Centre, CH Utrecht, Netherlands., Bunting DE; Centre for Integrative Systems Biology and Bioinformatics, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK., Sette A; Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037, USA., Kloetzel PM; Institut für Biochemie, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany.; Berlin Institute of Health, 10117 Berlin, Germany., Stumpf MP; Centre for Integrative Systems Biology and Bioinformatics, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK., Heck AJ; Biomolecular Mass Spectrometry and Proteomics, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CH Utrecht, Netherlands.; Netherlands Proteomics Centre, CH Utrecht, Netherlands., Mishto M; Institut für Biochemie, Charité-Universitätsmedizin Berlin, 10117 Berlin, Germany. michele.mishto@charite.de j.liepe@imperial.ac.uk.; Berlin Institute of Health, 10117 Berlin, Germany. |
| المصدر: | Science (New York, N.Y.) [Science] 2016 Oct 21; Vol. 354 (6310), pp. 354-358. Date of Electronic Publication: 2016 Oct 20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Original Publication: New York, N.Y. : [s.n.] 1880- |
| مواضيع طبية MeSH: | Antigen Presentation*, CD8-Positive T-Lymphocytes/*immunology , Epitopes, T-Lymphocyte/*metabolism , Histocompatibility Antigens Class I/*metabolism , Proteasome Endopeptidase Complex/*metabolism, Cell Line, Tumor ; Computational Biology ; Humans ; Ligands ; Peptides/immunology ; Peptides/metabolism ; Protein Splicing |
| مستخلص: | The proteasome generates the epitopes presented on human leukocyte antigen (HLA) class I molecules that elicit CD8 + T cell responses. Reports of proteasome-generated spliced epitopes exist, but they have been regarded as rare events. Here, however, we show that the proteasome-generated spliced peptide pool accounts for one-third of the entire HLA class I immunopeptidome in terms of diversity and one-fourth in terms of abundance. This pool also represents a unique set of antigens, possessing particular and distinguishing features. We validated this observation using a range of complementary experimental and bioinformatics approaches, as well as multiple cell types. The widespread appearance and abundance of proteasome-catalyzed peptide splicing events has implications for immunobiology and autoimmunity theories and may provide a previously untapped source of epitopes for use in vaccines and cancer immunotherapy. (Copyright © 2016, American Association for the Advancement of Science.) |
| معلومات مُعتمدة: | BB/G007934/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; NC/K001949/1 United Kingdom NC3RS_ National Centre for the Replacement, Refinement and Reduction of Animals in Research |
| سلسلة جزيئية: | Dryad 10.5061/dryad.r984n |
| المشرفين على المادة: | 0 (Epitopes, T-Lymphocyte) 0 (Histocompatibility Antigens Class I) 0 (Ligands) 0 (Peptides) EC 3.4.25.1 (Proteasome Endopeptidase Complex) |
| تواريخ الأحداث: | Date Created: 20161116 Date Completed: 20170407 Latest Revision: 20220129 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1126/science.aaf4384 |
| PMID: | 27846572 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1095-9203 |
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| DOI: | 10.1126/science.aaf4384 |