دورية أكاديمية
[Analysis of the protein expression differences between colonized and infectious multidrug-resistant acinetobacter baumannii].
| العنوان: | [Analysis of the protein expression differences between colonized and infectious multidrug-resistant acinetobacter baumannii]. |
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| المؤلفون: | Sui WJ; Department of Clinical Laboratory, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China., Wang M, Huang YF, Lu XX |
| المصدر: | Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2016 Nov 08; Vol. 96 (41), pp. 3319-3323. |
| نوع المنشور: | Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhonghua yi xue hui Country of Publication: China NLM ID: 7511141 Publication Model: Print Cited Medium: Print ISSN: 0376-2491 (Print) Linking ISSN: 03762491 NLM ISO Abbreviation: Zhonghua Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhonghua yi xue hui Original Publication: Beijing : Zhonghua yi xue hui. |
| مواضيع طبية MeSH: | Acinetobacter baumannii* , Drug Resistance, Bacterial* , Drug Resistance, Multiple* , Proteomics*, Acinetobacter Infections ; Cluster Analysis ; Hospitals ; Humans ; Membrane Proteins ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| مستخلص: | Objective: To analyze the protein expression differences between colonized multidrug-resistant acinetobacter baumannii (MDR-AB) and infectious MDR-AB using comparative proteomics and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and to explore the molecular characteristics of MDR-AB infections. Methods: A total of 59 MDR-AB strains including 23 of bloodstream infections MDR-AB and 36 of nasal, pharyngeal or environmental colonization MDR-AB were collected from Beijing Tongren Hospital between January 2008 and December 2011. MALDI-TOF MS cluster analysis was applied to identify the differences of protein expression between infection strains and colonization strains (including patient colonization and environmental colonization) of MDR-AB. Two-dimensional protein differences electrophoresis and MALDI-TOF-TOF MS were used to analyze the protein expression differences between bloodstream infection strain and nasal colonization strain isolated from the same patient. Results: ST92 was the main multiple locus sequence typing of 23 bloodstream infections MDR-AB. Cluster analysis showed that there were differences of protein fingerprint of MADLI-TOF MS between colonized and infectious MDR-AB. Proteomic differences were found between colonization strain and infection strain which were isolated from the same patient. And 9 major different protein spots were identified. They were trigger factor (score of identification was 10 218 and coverage rate was 44%), inosine-5'-monophosphate dehydrogenase (score of identification was 1 919 and coverage rate was 60%), phosphoglycerate kinase (score of identification was 947 and coverage rate was 49%), outer membrane protein 38 (score of identification was 572 and coverage rate was 23%) and so on. Conclusion: There were differences of protein expression between MDR-AB infection strains and colonization strains. And such different proteins could provide experimental evidence for molecular markers research of MDR-AB infections. |
| المشرفين على المادة: | 0 (Membrane Proteins) |
| تواريخ الأحداث: | Date Created: 20161118 Date Completed: 20180301 Latest Revision: 20181023 |
| رمز التحديث: | 20240628 |
| DOI: | 10.3760/cma.j.issn.0376-2491.2016.41.009 |
| PMID: | 27852378 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0376-2491 |
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| DOI: | 10.3760/cma.j.issn.0376-2491.2016.41.009 |