دورية أكاديمية
A novel Foxn1 eGFP/+ mouse model identifies Bmp4-induced maintenance of Foxn1 expression and thymic epithelial progenitor populations.
| العنوان: | A novel Foxn1 eGFP/+ mouse model identifies Bmp4-induced maintenance of Foxn1 expression and thymic epithelial progenitor populations. |
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| المؤلفون: | Barsanti M; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Lim JM; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Hun ML; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Lister N; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Wong K; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Hammett MV; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Lepletier A; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Boyd RL; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Giudice A; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia., Chidgey AP; Department of Anatomy and Developmental Biology, Monash University, Melbourne, Victoria, Australia. |
| المصدر: | European journal of immunology [Eur J Immunol] 2017 Feb; Vol. 47 (2), pp. 291-304. Date of Electronic Publication: 2016 Dec 07. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4141 (Electronic) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2005->: Weinheim : Wiley-VCH Original Publication: Weinheim, Verlag Chemie GmbH. |
| مواضيع طبية MeSH: | Aging/*physiology , Epithelial Cells/*physiology , Forkhead Transcription Factors/*metabolism , Stem Cells/*physiology , Thymus Gland/*physiology, Animals ; Bone Morphogenetic Protein 4/metabolism ; Bone Morphogenetic Protein Receptors, Type II/genetics ; Bone Morphogenetic Protein Receptors, Type II/metabolism ; Cells, Cultured ; Forkhead Transcription Factors/genetics ; Gene Expression Regulation ; Green Fluorescent Proteins/genetics ; Homeostasis ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Transgenic ; Models, Animal ; Thymus Gland/cytology |
| مستخلص: | Although forkhead-box n1 (Foxn1) is a critical thymic epithelial cell regulator in thymus organogenesis, its association with epithelial differentiation and homeostasis in the postnatal and aged thymic microenvironment remains conflicting. Consequently, we have generated a Foxn1 eGFP/+ knock-in mouse model that allows for refined investigation of the aging thymic epithelium. This reporter line differs from those previously published in that concomitant expression of enhanced green fluorescent protein enables live cell sorting of Foxn1 + cell populations. Our heterozygotes did not exhibit haploinsufficiency, with Foxn1 expression resembling that of wild-type mice. Comparative analysis between Foxn1 and enhanced green fluorescent protein at both the transcriptional and translational levels revealed co-localization, with progressive down-regulation observed predominantly in the aging cortical epithelium. Supplementation with bone morphogenetic protein (Bmp)-4 enhanced Foxn1 expression and colony forming efficiency in both embryonic and adult progenitor 3D cultures. Strikingly, selective maintenance of immature cortical and medullary epithelial cells was observed which is consistent with the higher Bmp receptor 2 expression levels seen in these progenitor populations. This study demonstrates the significance of our mouse model in unraveling the role of this master regulator in thymus development, homeostasis and aging, providing a faithful reporter system for phenotypic and functional investigations. (© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.) |
| فهرسة مساهمة: | Keywords: Aging; Bmp4; Foxn1; Foxn1eGFP mouse model; Thymic epithelial cell; Thymic epithelial progenitor cell; Thymus development |
| المشرفين على المادة: | 0 (Bmp4 protein, mouse) 0 (Bone Morphogenetic Protein 4) 0 (Forkhead Transcription Factors) 0 (Whn protein) 147336-22-9 (Green Fluorescent Proteins) EC 2.7.11.30 (Bone Morphogenetic Protein Receptors, Type II) |
| تواريخ الأحداث: | Date Created: 20161119 Date Completed: 20170705 Latest Revision: 20171202 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/eji.201646553 |
| PMID: | 27861793 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1521-4141 |
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| DOI: | 10.1002/eji.201646553 |