دورية أكاديمية
أعرض في EDS

Discarded Livers Find a New Life: Engineered Liver Grafts Using Hepatocytes Recovered From Marginal Livers.

التفاصيل البيبلوغرافية
العنوان: Discarded Livers Find a New Life: Engineered Liver Grafts Using Hepatocytes Recovered From Marginal Livers.
المؤلفون: Uygun BE; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA., Izamis ML; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA., Jaramillo M; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA., Chen Y; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA., Price G; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA., Ozer S; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA., Yarmush ML; Department of Surgery, Center for Engineering in Medicine, Massachusetts General Hospital, Harvard Medical School, and the Shriners Burns Hospital, Boston, MA.; Department of Biomedical Engineering, Rutgers University, Piscataway, NJ, USA.
المصدر: Artificial organs [Artif Organs] 2017 Jun; Vol. 41 (6), pp. 579-585. Date of Electronic Publication: 2016 Nov 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 7802778 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-1594 (Electronic) Linking ISSN: 0160564X NLM ISO Abbreviation: Artif Organs Subsets: MEDLINE
أسماء مطبوعة: Publication: Cambridge, MA : Wiley-Blackwell
Original Publication: Cleveland, International Society for Artificial Organs.
مواضيع طبية MeSH: Liver Regeneration*, Hepatocytes/*cytology , Liver/*physiology , Tissue Engineering/*methods, Animals ; Cell Separation ; Cells, Cultured ; Extracellular Matrix/chemistry ; Liver/chemistry ; Liver/cytology ; Perfusion ; Rats ; Tissue Scaffolds/chemistry
مستخلص: Treatment for end-stage liver failure is restricted by the critical shortage of donor organs; about 4000 people die in the USA while waiting for a transplantable organ. This situation has been a major driving force behind the rise of tissue engineering to build artificial tissues/organs. Recent advancements in creating transplantable liver grafts using decellularized liver scaffolds bring the field closer to clinical translation. However, a source of readily available and highly functional adult hepatocytes in adequate numbers for regenerative liver therapies still remains unclear. Here, we describe a new method to utilize discarded livers to make transplantable new liver grafts. We show that marginal donor livers damaged due to warm ischemia could be treated with machine perfusion to yield 39 million viable hepatocytes per gram of liver, similar to fresh livers, and these cells could be used to repopulate decellularized liver matrix (DLM) scaffolds to make transplantable liver grafts. The hepatocytes from recovered livers sustained their characteristic epithelial morphology while they exhibited slightly lower protein synthesis functions both in plate cultures and in recellularized liver grafts. The dampened protein synthesis was attributed to residual endoplasmic reticulum stress found in recovered cells. The results here represent a unique approach to reengineer transplantable liver grafts solely from discarded organs.
(© 2016 The Authors Artificial Organs published by Wiley Periodicals, Inc. on behalf of International Center for Artificial Organ and Transplantation (ICAOT).)
References: Biotechnol Prog. 1991 May-Jun;7(3):237-45. (PMID: 1367596)
J Pathol. 2005 Sep;207(1):111-8. (PMID: 15912576)
Resuscitation. 2005 Sep;66(3):379-89. (PMID: 16029920)
Stem Cells. 2006 Oct;24(10):2244-51. (PMID: 16778153)
Science. 2006 Aug 25;313(5790):1137-40. (PMID: 16931765)
Transplantation. 2009 Jan 27;87(2):170-7. (PMID: 19155970)
Int J Cell Biol. 2010;2010:830307. (PMID: 20169136)
Nat Med. 2010 Jul;16(7):814-20. (PMID: 20543851)
Methods Enzymol. 2011;490:71-92. (PMID: 21266244)
J Vis Exp. 2011 Feb 04;(48):null. (PMID: 21339718)
Annu Rev Biomed Eng. 2011 Aug 15;13:27-53. (PMID: 21417722)
PLoS One. 2011 Mar 29;6(3):e18222. (PMID: 21479238)
J Surg Res. 2012 Jun 1;175(1):149-56. (PMID: 21550058)
PLoS One. 2013 Jul 16;8(7):e69334. (PMID: 23874947)
PLoS One. 2013 Jul 26;8(7):e69758. (PMID: 23922793)
Biotechnol Adv. 2014 Mar-Apr;32(2):504-13. (PMID: 24440487)
PLoS One. 2014 Jul 07;9(7):e101386. (PMID: 24999631)
Cell Med. 2013;4(3):109-123. (PMID: 25431743)
Stem Cells Transl Med. 2015 Mar;4(3):207-10. (PMID: 25637191)
Cryobiology. 2015 Oct;71(2):244-55. (PMID: 26188080)
Transplantation. 2016 Feb;100(2):446-50. (PMID: 26516669)
J Clin Transl Res. 2015 Jul 19;1(1):48-56. (PMID: 30873444)
Biochem J. 1972 Sep;129(2):3P. (PMID: 4643325)
Microsurgery. 1996;17(12):690-6. (PMID: 9588714)
معلومات مُعتمدة: K99 DK088962 United States DK NIDDK NIH HHS; R00 DK088962 United States DK NIDDK NIH HHS; R01 DK084053 United States DK NIDDK NIH HHS
فهرسة مساهمة: Keywords: -Endoplasmic reticulum stress; -Hepatocytes; -Liver recellularization; Machine perfusion
تواريخ الأحداث: Date Created: 20161119 Date Completed: 20171012 Latest Revision: 20220409
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5702548
DOI: 10.1111/aor.12781
PMID: 27862079
قاعدة البيانات: MEDLINE
الوصف
تدمد:1525-1594
DOI:10.1111/aor.12781