دورية أكاديمية
T Cell Responses and Regulation and the Impact of In Vitro IL-10 and TGF-β Modulation During Treatment of Active Tuberculosis.
| العنوان: | T Cell Responses and Regulation and the Impact of In Vitro IL-10 and TGF-β Modulation During Treatment of Active Tuberculosis. |
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| المؤلفون: | Feruglio SL; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.; Norwegian Institute of Public Health, Oslo, Norway., Kvale D; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.; Department of Infectious Diseases, Oslo University Hospital, Oslo, Norway.; K. G. Jebsen Inflammation Research Center, University of Oslo, Oslo, Norway., Dyrhol-Riise AM; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.; Department of Infectious Diseases, Oslo University Hospital, Oslo, Norway.; K. G. Jebsen Inflammation Research Center, University of Oslo, Oslo, Norway.; Department of Clinical Science, University of Bergen, Bergen, Norway. |
| المصدر: | Scandinavian journal of immunology [Scand J Immunol] 2017 Feb; Vol. 85 (2), pp. 138-146. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Blackwell Scientific Publications Country of Publication: England NLM ID: 0323767 Publication Model: Print Cited Medium: Internet ISSN: 1365-3083 (Electronic) Linking ISSN: 03009475 NLM ISO Abbreviation: Scand J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford : Blackwell Scientific Publications Original Publication: Oslo, Universitetsforlaget. |
| مواضيع طبية MeSH: | Interleukin-10/*immunology , Mycobacterium tuberculosis/*immunology , T-Lymphocytes/*immunology , Transforming Growth Factor beta/*immunology , Tuberculosis/*immunology, Adult ; Aged ; Aged, 80 and over ; Antitubercular Agents/therapeutic use ; Cell Proliferation/drug effects ; Female ; Flow Cytometry ; Host-Pathogen Interactions/drug effects ; Host-Pathogen Interactions/immunology ; Humans ; Interleukin-10/metabolism ; Lymphocyte Activation/drug effects ; Lymphocyte Activation/immunology ; Male ; Middle Aged ; Mycobacterium tuberculosis/drug effects ; Mycobacterium tuberculosis/physiology ; Outcome Assessment, Health Care ; T-Lymphocytes/drug effects ; T-Lymphocytes/metabolism ; T-Lymphocytes, Regulatory/drug effects ; T-Lymphocytes, Regulatory/immunology ; T-Lymphocytes, Regulatory/metabolism ; Time Factors ; Transforming Growth Factor beta/metabolism ; Tuberculosis/drug therapy ; Tuberculosis/microbiology ; Young Adult |
| مستخلص: | Mycobacterium tuberculosis (Mtb) is particularly challenging for the immune system being an intracellular pathogen, and a variety of T cell subpopulations are activated by the host defence mechanism. In this study, we investigated T cell responses and regulation in active TB patients with drug-sensitive Mtb (N = 18) during 24 weeks of efficient anti-TB therapy. T cell activation, differentiation, regulatory T cell (Treg) subsets, Mtb-induced T cell proliferation and in vitro IL-10 and TGF-β modulation were analysed by flow cytometry at baseline and after 8 and 24 weeks of therapy, while soluble cytokines in culture supernatants were analysed by a 9-plex Luminex assay. Successful treatment resulted in significantly reduced co-expression of HLA-DR/CD38 and PD-1/CD38 on both CD4 + and CD8 + T cells, while the fraction of CD4 + CD25 high CD127 low Tregs (P = 0.017) and CD4 + CD25 high CD127 low CD147 + Tregs (P = 0.029) showed significant transient increase at week 8. In vitro blockade of IL-10/TGF-β upon Mtb antigen stimulation significantly lowered the fraction of ESAT-6-specific CD4 + CD25 high CD127 low Tregs at baseline (P = 0.047), while T cell proliferation and cytokine production were unaffected. Phenotypical and Mtb-specific T cell signatures may serve as markers of effective therapy, while the IL-10/TGF-β pathway could be a target for early inhibition to facilitate Mtb clearance. However, larger clinical studies are needed for verification before concluding. (© 2016 The Authors. Scandinavian Journal of Immunology published by John Wiley & Sons Ltd on behalf of The Foundation for the Scandinavian Journal of Immunology.) |
| المشرفين على المادة: | 0 (Antitubercular Agents) 0 (Transforming Growth Factor beta) 130068-27-8 (Interleukin-10) |
| تواريخ الأحداث: | Date Created: 20161119 Date Completed: 20170405 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/sji.12511 |
| PMID: | 27862137 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1365-3083 |
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| DOI: | 10.1111/sji.12511 |