دورية أكاديمية
أعرض في EDS

Human regulatory B cells control the T FH cell response.

التفاصيل البيبلوغرافية
العنوان: Human regulatory B cells control the T FH cell response.
المؤلفون: Achour A; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France., Simon Q; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France., Mohr A; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France., Séité JF; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France; CHRU Morvan, Laboratoire d'Immunologie, Brest, France., Youinou P; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France; CHRU Morvan, Laboratoire d'Immunologie, Brest, France., Bendaoud B; CHRU Morvan, Laboratoire d'Immunologie, Brest, France., Ghedira I; Faculty of Pharmacy, Department of Immunology, Research Unit (03/UR/07-02), Monastir, Tunisia., Pers JO; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France; CHRU Morvan, Laboratoire d'Immunologie, Brest, France., Jamin C; Université de Brest, INSERM U1227, Lymphocytes B et Autoimmunité, and LabEx IGO, Brest, France; CHRU Morvan, Laboratoire d'Immunologie, Brest, France. Electronic address: christophe.jamin@univ-brest.fr.
المصدر: The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 2017 Jul; Vol. 140 (1), pp. 215-222. Date of Electronic Publication: 2016 Nov 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Mosby Country of Publication: United States NLM ID: 1275002 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-6825 (Electronic) Linking ISSN: 00916749 NLM ISO Abbreviation: J Allergy Clin Immunol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: St Louis, Mosby.
مواضيع طبية MeSH: B-Lymphocytes, Regulatory/*immunology , T-Lymphocytes, Helper-Inducer/*immunology, B-Lymphocytes, Regulatory/physiology ; Cell Differentiation ; Cells, Cultured ; Coculture Techniques ; Humans ; Interleukin-12/immunology ; Interleukins/immunology ; T-Lymphocytes, Helper-Inducer/physiology
مستخلص: Background: Follicular helper T (T FH ) cells support terminal B-cell differentiation. Human regulatory B (Breg) cells modulate cellular responses, but their control of T FH cell-dependent humoral immune responses is unknown.
Objective: We sought to assess the role of Breg cells on T FH cell development and function.
Methods: Human T cells were polyclonally stimulated in the presence of IL-12 and IL-21 to generate T FH cells. They were cocultured with B cells to induce their terminal differentiation. Breg cells were included in these cultures, and their effects were evaluated by using flow cytometry and ELISA.
Results: B-cell lymphoma 6, IL-21, inducible costimulator, CXCR5, and programmed cell death protein 1 (PD-1) expressions increased on stimulated human T cells, characterizing T FH cell maturation. In cocultures they differentiated B cells into CD138 + plasma and IgD - CD27 + memory cells and triggered immunoglobulin secretions. Breg cells obtained by Toll-like receptor 9 and CD40 activation of B cells prevented T FH cell development. Added to T FH cell and B-cell cocultures, they inhibited B-cell differentiation, impeded immunoglobulin secretions, and expanded Foxp3 + CXCR5 + PD-1 + follicular regulatory T cells. Breg cells modulated IL-21 receptor expressions on T FH cells and B cells, and their suppressive activities involved CD40, CD80, CD86, and intercellular adhesion molecule interactions and required production of IL-10 and TGF-β.
Conclusion: Human Breg cells control T FH cell maturation, expand follicular regulatory T cells, and inhibit the T FH cell-mediated antibody secretion. These novel observations demonstrate a role for the Breg cell in germinal center reactions and suggest that deficient activities might impair the T FH cell-dependent control of humoral immunity and might lead to the development of aberrant autoimmune responses.
(Copyright © 2016 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: IL-12; IL-21; Regulatory B cells; antibody production; follicular T helper cells; humoral immune response
المشرفين على المادة: 0 (Interleukins)
187348-17-0 (Interleukin-12)
MKM3CA6LT1 (interleukin-21)
تواريخ الأحداث: Date Created: 20161121 Date Completed: 20170829 Latest Revision: 20181202
رمز التحديث: 20221213
DOI: 10.1016/j.jaci.2016.09.042
PMID: 27865860
قاعدة البيانات: MEDLINE
الوصف
تدمد:1097-6825
DOI:10.1016/j.jaci.2016.09.042