دورية أكاديمية
An Opsonic Phagocytosis Assay for Plasmodium falciparum Sporozoites.
| العنوان: | An Opsonic Phagocytosis Assay for Plasmodium falciparum Sporozoites. |
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| المؤلفون: | Steel RW; Center for Infectious Disease Research, Seattle, Washington, USA., Sack BK; Center for Infectious Disease Research, Seattle, Washington, USA., Tsuji M; HIV and Malaria Vaccine Program, Aaron Diamond AIDS Research Center, New York, New York, USA., Navarro MJ; Center for Infectious Disease Research, Seattle, Washington, USA., Betz W; Center for Infectious Disease Research, Seattle, Washington, USA., Fishbaugher ME; Center for Infectious Disease Research, Seattle, Washington, USA., Flannery EL; Center for Infectious Disease Research, Seattle, Washington, USA., Kappe SH; Center for Infectious Disease Research, Seattle, Washington, USA Stefan.Kappe@cidresearch.org.; Department of Global Health, University of Washington, Seattle, Washington, USA. |
| المصدر: | Clinical and vaccine immunology : CVI [Clin Vaccine Immunol] 2017 Feb 06; Vol. 24 (2). Date of Electronic Publication: 2017 Feb 06 (Print Publication: 2017). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Microbiology Country of Publication: United States NLM ID: 101252125 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1556-679X (Electronic) Linking ISSN: 1556679X NLM ISO Abbreviation: Clin Vaccine Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Society for Microbiology, [2006]- |
| مواضيع طبية MeSH: | Phagocytosis*, Antibodies, Protozoan/*blood , Immunoassay/*methods , Opsonin Proteins/*blood , Plasmodium falciparum/*immunology , Sporozoites/*immunology, Flow Cytometry/methods ; Humans |
| مستخلص: | Plasmodium falciparum malaria remains the deadliest parasitic disease worldwide. Vaccines targeting the preerythrocytic sporozoite and liver stages have the potential to entirely prevent blood-stage infection and disease, as well as onward transmission. Sporozoite surface and secreted proteins are leading candidates for inclusion in a preerythrocytic stage-specific, antibody-based vaccine. Preclinical functional assays to identify humoral correlates of protection in vitro and to validate novel sporozoite protein targets for inclusion in multisubunit vaccines currently do not consider the interaction of sporozoite-targeting antibodies with other components of the immune system. Here, we describe the development of a simple flow cytometric assay to quantitatively assess the ability of antibodies directed against P. falciparum sporozoites to facilitate their phagocytosis. We demonstrate that this sporozoite opsonic phagocytosis assay (SOPA) is compatible with both monoclonal antibodies and human immune serum and can be performed using cryopreserved P. falciparum sporozoites. This simple, accessible assay will aid with the assessment of antibody responses to vaccination with Plasmodium antigens and their interaction with phagocytic cells of the immune system. (Copyright © 2017 American Society for Microbiology.) |
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| معلومات مُعتمدة: | F32 AI114113 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Plasmodium falciparum; antibodies; assay development; malaria; opsonization; phagocytosis; sporozoite; vaccines |
| المشرفين على المادة: | 0 (Antibodies, Protozoan) 0 (Opsonin Proteins) |
| تواريخ الأحداث: | Date Created: 20161125 Date Completed: 20170830 Latest Revision: 20181113 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5299119 |
| DOI: | 10.1128/CVI.00445-16 |
| PMID: | 27881488 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1556-679X |
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| DOI: | 10.1128/CVI.00445-16 |