دورية أكاديمية
Essential role of K + uptake permease (Kup) for resistance to sucrose-induced stress in Gluconacetobacter diazotrophicus PAl 5.
| العنوان: | Essential role of K + uptake permease (Kup) for resistance to sucrose-induced stress in Gluconacetobacter diazotrophicus PAl 5. |
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| المؤلفون: | de Oliveira MV; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, UENF, Campos dos Goytacazes, RJ, Brazil., Intorne AC; Laboratório de Fisiologia e Bioquímica de Microrganismos, Universidade Estadual do Norte Fluminense Darcy Ribeiro, UENF, Campos dos Goytacazes, RJ, Brazil., Vespoli LS; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, UENF, Campos dos Goytacazes, RJ, Brazil., Andrade LF; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, UENF, Campos dos Goytacazes, RJ, Brazil., Pereira LM; Laboratório de Biologia Parasitária, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Rio Grande do Sul, Brazil., Rangel PL; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, UENF, Campos dos Goytacazes, RJ, Brazil., de Souza Filho GA; Laboratório de Biotecnologia, Universidade Estadual do Norte Fluminense Darcy Ribeiro, UENF, Campos dos Goytacazes, RJ, Brazil. |
| المصدر: | Environmental microbiology reports [Environ Microbiol Rep] 2017 Apr; Vol. 9 (2), pp. 85-90. Date of Electronic Publication: 2017 Jan 31. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Society for Applied Microbiology and Blackwell Pub Country of Publication: United States NLM ID: 101499207 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1758-2229 (Electronic) Linking ISSN: 17582229 NLM ISO Abbreviation: Environ Microbiol Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Hoboken, N.J.] : Society for Applied Microbiology and Blackwell Pub., c2009- |
| مواضيع طبية MeSH: | Osmotic Pressure* , Stress, Physiological*, Gluconacetobacter/*drug effects , Gluconacetobacter/*physiology , Membrane Transport Proteins/*metabolism , Potassium/*metabolism , Sucrose/*metabolism, DNA Transposable Elements ; Genetic Complementation Test ; Gluconacetobacter/genetics ; Mutagenesis, Insertional |
| مستخلص: | Microorganisms are constantly challenged by stressful conditions, such as sugar-rich environments. Such environments can cause an imbalance of biochemical activities and compromise cell multiplication. Gluconacetobacter diazotrophicus PAl 5 is among the most sugar-tolerant bacteria, capable of growing in the presence of up to 876 mM sucrose. However, the molecular mechanisms involved in its response to high sucrose remain unknown. The present work aimed to identify sucrose-induced stress resistance genes in G. diazotrophicus PAl 5. Screening of a Tn5 transposon insertion library identified a mutant that was severely compromised in its resistance to high sucrose concentrations. Molecular characterization revealed that the mutation affected the kupA gene, which encodes a K + uptake transporter (KupA). Functional complementation of the mutant with the wild type kupA gene recovered the sucrose-induced stress resistance phenotype. High sucrose resistance assay, under different potassium concentrations, revealed that KupA acts as a high-affinity K + transporter, which is essential for resistance to sucrose-induced stress, when extracellular potassium levels are low. This study is the first to show the essential role of the KupA protein for resistance to sucrose-induced stress in bacteria by acting as a high-affinity potassium transporter in G. diazotrophicus PAl 5. (© 2016 Society for Applied Microbiology and John Wiley & Sons Ltd.) |
| المشرفين على المادة: | 0 (DNA Transposable Elements) 0 (Membrane Transport Proteins) 57-50-1 (Sucrose) RWP5GA015D (Potassium) |
| تواريخ الأحداث: | Date Created: 20161126 Date Completed: 20170829 Latest Revision: 20171127 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/1758-2229.12503 |
| PMID: | 27886654 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1758-2229 |
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| DOI: | 10.1111/1758-2229.12503 |