Promethazine improves antibiotic efficacy and disrupts biofilms of Burkholderia pseudomallei.

التفاصيل البيبلوغرافية
العنوان:	Promethazine improves antibiotic efficacy and disrupts biofilms of Burkholderia pseudomallei.
المؤلفون:	Sidrim JJ; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Vasconcelos DC; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Riello GB; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Guedes GM; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Serpa R; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Bandeira TJ; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Monteiro AJ; b Department of Statistics and Applied Mathematics , Federal University of Ceará , Fortaleza , Brazil., Cordeiro RA; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Castelo-Branco DS; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil., Rocha MF; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil.; c Postgraduate Program in Veterinary Science , State University of Ceará , Fortaleza , Brazil., Brilhante RS; a Specialized Center of Medical Micology , Federal University of Ceará , Fortaleza , Brazil.
المصدر:	Biofouling [Biofouling] 2017 Jan; Vol. 33 (1), pp. 88-97. Date of Electronic Publication: 2016 Dec 12.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Taylor & Francis Country of Publication: England NLM ID: 9200331 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1029-2454 (Electronic) Linking ISSN: 08927014 NLM ISO Abbreviation: Biofouling Subsets: MEDLINE
أسماء مطبوعة:	Publication: 2002- : Abingdon, Oxford : Taylor & Francis Original Publication: Chur ; New York : Harwood Academic Publishers, c1988-
مواضيع طبية MeSH:	Anti-Bacterial Agents/pharmacology , Biofilms/drug effects , Burkholderia pseudomallei/drug effects , Promethazine/pharmacology, Burkholderia pseudomallei/physiology ; Drug Synergism ; Microbial Sensitivity Tests ; Plankton/drug effects
مستخلص:	Efflux pumps are important defense mechanisms against antimicrobial drugs and maintenance of Burkholderia pseudomallei biofilms. This study evaluated the effect of the efflux pump inhibitor promethazine on the structure and antimicrobial susceptibility of B. pseudomallei biofilms. Susceptibility of planktonic cells and biofilms to promethazine alone and combined with antimicrobials was assessed by the broth microdilution test and biofilm metabolic activity was determined with resazurin. The effect of promethazine on 48 h-grown biofilms was also evaluated through confocal and electronic microscopy. The minimum inhibitory concentration (MIC) of promethazine was 780 mg l ^-1 , while the minimum biofilm elimination concentration (MBEC) was 780-3,120 mg l ^-1 . Promethazine reduced the MIC values for erythromycin, trimethoprim/sulfamethoxazole, gentamicin and ciprofloxacin and reduced the MBEC values for all tested drugs (p<0.05). Microscopic analyses demonstrated that promethazine altered the biofilm structure of B. pseudomallei, even at subinhibitory concentrations, possibly facilitating antibiotic penetration. Promethazine improves antibiotics efficacy against B. pseudomallei biofilms, by disrupting biofilm structure.
فهرسة مساهمة:	Keywords: Burkholderia pseudomallei; biofilms; efflux pumps; promethazine; resistance; synergism
المشرفين على المادة:	0 (Anti-Bacterial Agents) FF28EJQ494 (Promethazine)
تواريخ الأحداث:	Date Created: 20161213 Date Completed: 20170828 Latest Revision: 20181202
رمز التحديث:	20221213
DOI:	10.1080/08927014.2016.1262846
PMID:	27936915
قاعدة البيانات:	MEDLINE

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تدمد:	1029-2454
DOI:	10.1080/08927014.2016.1262846