دورية أكاديمية
أعرض في EDS

Features of endothelial dysfunction and morphofunctional changes of the uteroplacental complex in experimentally induced pre-eclampsia.

التفاصيل البيبلوغرافية
العنوان: Features of endothelial dysfunction and morphofunctional changes of the uteroplacental complex in experimentally induced pre-eclampsia.
المؤلفون: Tyurenkov IN; Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia., Perfilova VN; Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia. Electronic address: vnperfilova@gmail.com., Smirnov AV; Department of Pathological Anatomy, Volgograd State Medical University, Volgograd, Russia., Reznikova LB; Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia., Poroyskaya AV; Department of Pharmacology and Biopharmacy of the Department for Refresher Training, Volgograd State Medical University, 400001 Volgograd, Russia., Verovsky VE; Department of Theoretical Biochemistry with a Course of Clinical Biochemistry, Volgograd State Medical University, Volgograd, Russia.
المصدر: Pregnancy hypertension [Pregnancy Hypertens] 2016 Oct; Vol. 6 (4), pp. 423-430. Date of Electronic Publication: 2016 Nov 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101552483 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2210-7797 (Electronic) Linking ISSN: 22107789 NLM ISO Abbreviation: Pregnancy Hypertens Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amtserdam : Elsevier
مواضيع طبية MeSH: Disease Models, Animal*, Endothelium/*physiopathology , Endothelium, Vascular/*physiopathology , Placenta/*physiopathology , Pre-Eclampsia/*physiopathology , Uterus/*physiopathology, Animals ; Arterial Pressure ; Body Water ; Female ; Nitric Oxide Synthase Type II/metabolism ; Nitric Oxide Synthase Type III/metabolism ; Placenta/blood supply ; Placenta/pathology ; Placental Circulation ; Pre-Eclampsia/chemically induced ; Pregnancy ; Proteinuria/etiology ; Rats, Wistar ; Sodium Chloride ; Uterus/blood supply ; Uterus/pathology ; Vascular Endothelial Growth Factor A/metabolism ; Vasodilation
مستخلص: Background: Pre-eclampsia is considered to be a severe complication of pregnancy. Theoretical investigation of its etiology and pathogenesis, development of strategies for its prevention and treatment are conditioned by the development of appropriate experimental models of this pathology.
Methods: The study involved Wistar rat lines weighing 220-240g. Experimental pre-eclampsia was modeled by replacing drinking water consumed by pregnant female rats with 1.8% NaCl solution throughout gestation. Arterial pressure, protein concentration in urine and tissue hydration extent were measured on the 1st and 21st days of gestation. Uteroplacental blood flow, vasodilating and antithrombotic endothelial functions were also assessed. For pathomorphological and immunohistochemical investigation murine monoclonal antibodies against vascular endothelial growth factor (VEGF), polyclonal rabbit antibodies against inducible and endothelial NO-synthases were used.
Results: Replacing drinking water with 1.8% NaCl solution in female rats throughout gestation elevates arterial pressure, causes proteinuria and edema, impairs vasodilating and antithrombotic endothelial properties, and suppresses uteroplacental blood circulation. A morphological examination of the animals revealed the signs of focal duodenitis, spasms of myometrium arteries with no invasion of syncytiotrophoblast into its walls which also involved a raised VEGF and reduced eNOS expression in the endothelium of myometrial vessels, as well as cytoplasmic expression of iNOS in the cells of inflammatory infiltrate.
Conclusions: These findings make it possible to conclude that replacing drinking water with 1.8% NaCl solution causes a number of changes typical of pre-eclampsia and, therefore, can be regarded as an experimental model of this pathologic condition.
(Copyright © 2016 International Society for the Study of Hypertension in Pregnancy. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Experimental model pre-eclampsia endothelial dysfunction; Morphology; Pharmacology
المشرفين على المادة: 0 (Vascular Endothelial Growth Factor A)
0 (vascular endothelial growth factor A, rat)
451W47IQ8X (Sodium Chloride)
EC 1.14.13.39 (Nitric Oxide Synthase Type II)
EC 1.14.13.39 (Nitric Oxide Synthase Type III)
تواريخ الأحداث: Date Created: 20161213 Date Completed: 20170417 Latest Revision: 20170417
رمز التحديث: 20231215
DOI: 10.1016/j.preghy.2016.11.001
PMID: 27939494
قاعدة البيانات: MEDLINE
الوصف
تدمد:2210-7797
DOI:10.1016/j.preghy.2016.11.001