دورية أكاديمية
Heterologous expression of Paranosema (Antonospora) locustae hexokinase in lepidopteran, Sf9, cells is followed by accumulation of the microsporidian protein in insect cell nuclei.
| العنوان: | Heterologous expression of Paranosema (Antonospora) locustae hexokinase in lepidopteran, Sf9, cells is followed by accumulation of the microsporidian protein in insect cell nuclei. |
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| المؤلفون: | Timofeev SA; Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia., Senderskiy IV; Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia., Tsarev AA; Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia., Tokarev YS; Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia., Dolgikh VV; Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia. Electronic address: dol1slav@yahoo.com. |
| المصدر: | Journal of invertebrate pathology [J Invertebr Pathol] 2017 Feb; Vol. 143, pp. 104-107. Date of Electronic Publication: 2016 Dec 16. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0014067 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0805 (Electronic) Linking ISSN: 00222011 NLM ISO Abbreviation: J Invertebr Pathol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York, NY : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Fungal Proteins/*biosynthesis , Hexokinase/*biosynthesis , Nosema/*enzymology , Spodoptera/*parasitology, Animals ; Cell Nucleus/metabolism ; Microsporidiosis/veterinary ; Pichia |
| مستخلص: | Paranosema (Nosema, Antonospora) locustae is the only microsporidium produced as a commercial product for biological control. Molecular mechanisms of the effects of this pathogen and other invertebrate microsporidia on host cells remain uncharacterized. Previously, we immunolocalized P. locustae hexokinase in nuclei of Locusta migratoria infected adipocytes. Here, the microsporidian protein was expressed in the yeast Pichia pastoris and in lepidopteran Sf9 cells. During heterologous expression, P. locustae hexokinase was accumulated in the nuclei of insect cells but not in yeast cell nuclei. This confirms nuclear localization of hexokinase secreted by microsporidia into infected host cells and suggests convenient model for its further study. (Copyright © 2016 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Heterologous expression; Hexokinase; Insect pathogens; Intracellular parasites; Microsporidia; Nuclear localization; Paranosema locustae; Secretory proteins |
| المشرفين على المادة: | 0 (Fungal Proteins) EC 2.7.1.1 (Hexokinase) |
| تواريخ الأحداث: | Date Created: 20161220 Date Completed: 20170926 Latest Revision: 20180321 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.jip.2016.12.002 |
| PMID: | 27989634 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-0805 |
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| DOI: | 10.1016/j.jip.2016.12.002 |