دورية أكاديمية
Molecular Dynamics Simulation and Experimental Studies of Gold Nanoparticle Templated HDL-like Nanoparticles for Cholesterol Metabolism Therapeutics.
| العنوان: | Molecular Dynamics Simulation and Experimental Studies of Gold Nanoparticle Templated HDL-like Nanoparticles for Cholesterol Metabolism Therapeutics. |
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| المؤلفون: | Lai CT; Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States., Sun W; Department of Urology, Northwestern University , Chicago, Illinois 60611, United States.; Simpson Querrey Institute for Bionanotechnology , 303 East Superior, Chicago, Illinois 60611, United States.; International Institute for Nanotechnology, 2145 Sheridan Road, Evanston, Illinois 60208, United States., Palekar RU; Department of Urology, Northwestern University , Chicago, Illinois 60611, United States.; Simpson Querrey Institute for Bionanotechnology , 303 East Superior, Chicago, Illinois 60611, United States.; International Institute for Nanotechnology, 2145 Sheridan Road, Evanston, Illinois 60208, United States., Thaxton CS; Department of Urology, Northwestern University , Chicago, Illinois 60611, United States.; Simpson Querrey Institute for Bionanotechnology , 303 East Superior, Chicago, Illinois 60611, United States.; International Institute for Nanotechnology, 2145 Sheridan Road, Evanston, Illinois 60208, United States., Schatz GC; Department of Chemistry, Northwestern University , Evanston, Illinois 60208, United States. |
| المصدر: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2017 Jan 18; Vol. 9 (2), pp. 1247-1254. Date of Electronic Publication: 2017 Jan 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Metal Nanoparticles*, Cholesterol ; Gold ; Lipoproteins, HDL ; Molecular Dynamics Simulation |
| مستخلص: | High-density lipoprotein (HDL) plays an important role in the transport and metabolism of cholesterol. Mimics of HDL are being explored as potentially powerful therapeutic agents for removing excess cholesterol from arterial plaques. Gold nanoparticles (AuNPs) functionalized with apolipoprotein A-I and with the lipids 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[3-(2-pyridyldithio)propionate] have been demonstrated to be robust acceptors of cellular cholesterol. However, detailed structural information about this functionalized HDL AuNP is still lacking. In this study, we have used X-ray photoelectron spectroscopy and lecithin/cholesterol acyltransferase activation experiments together with coarse-grained and all-atom molecular dynamics simulations to model the structure and cholesterol uptake properties of the HDL AuNP construct. By simulating different apolipoprotein-loaded AuNPs, we find that lipids are oriented differently in regions with and without apoA-I. We also show that in this functionalized HDL AuNP, the distribution of cholesteryl ester maintains a reverse concentration gradient that is similar to the gradient found in native HDL. |
| فهرسة مساهمة: | Keywords: HDL; LCAT; apolipoprotein A-I; cholesterol; cholesteryl ester; gold nanoparticle |
| المشرفين على المادة: | 0 (Lipoproteins, HDL) 7440-57-5 (Gold) 97C5T2UQ7J (Cholesterol) |
| تواريخ الأحداث: | Date Created: 20161222 Date Completed: 20181214 Latest Revision: 20181214 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acsami.6b12249 |
| PMID: | 28001031 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1944-8252 |
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| DOI: | 10.1021/acsami.6b12249 |