دورية أكاديمية
Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold.
| العنوان: | Recellularization via the bile duct supports functional allogenic and xenogenic cell growth on a decellularized rat liver scaffold. |
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| المؤلفون: | Hassanein W; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Uluer MC; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Langford J; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Woodall JD; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Cimeno A; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Dhru U; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Werdesheim A; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Harrison J; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Rivera-Pratt C; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Klepfer S; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Khalifeh A; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Buckingham B; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Brazio PS; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Parsell D; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Klassen C; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., Drachenberg C; b University of Maryland School of Medicine , Department of Pathology , Baltimore , MD , USA., Barth RN; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA., LaMattina JC; a University of Maryland School of Medicine, Department of Surgery , Division of Transplantation , Baltimore , MD , USA. |
| المصدر: | Organogenesis [Organogenesis] 2017 Jan 02; Vol. 13 (1), pp. 16-27. Date of Electronic Publication: 2016 Dec 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: United States NLM ID: 101253266 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1555-8592 (Electronic) Linking ISSN: 15476278 NLM ISO Abbreviation: Organogenesis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Philadelphia, PA : Taylor & Francis Original Publication: Georgetown, TX : Landes Bioscience |
| مواضيع طبية MeSH: | Bile Ducts/*cytology , Liver/*cytology , Tissue Scaffolds/*chemistry, Albumins/metabolism ; Animals ; Animals, Newborn ; Cell Compartmentation ; Cell Lineage ; Cell Proliferation ; Cell Tracking ; DNA/metabolism ; Hep G2 Cells ; Human Umbilical Vein Endothelial Cells/metabolism ; Humans ; Male ; Rats, Wistar ; Reproducibility of Results |
| مستخلص: | Recent years have seen a proliferation of methods leading to successful organ decellularization. In this experiment we examine the feasibility of a decellularized liver construct to support growth of functional multilineage cells. Bio-chamber systems were used to perfuse adult rat livers with 0.1% SDS for 24 hours yielding decellularized liver scaffolds. Initially, we recellularized liver scaffolds using a human tumor cell line (HepG2, introduced via the bile duct). Subsequent studies were performed using either human tumor cells co-cultured with human umbilical vein endothelial cells (HUVECs, introduced via the portal vein) or rat neonatal cell slurry (introduced via the bile duct). Bio-chambers were used to circulate oxygenated growth medium via the portal vein at 37C for 5-7 days. Human HepG2 cells grew readily on the scaffold (n = 20). HepG2 cells co-cultured with HUVECs demonstrated viable human endothelial lining with concurrent hepatocyte growth (n = 10). In the series of neonatal cell slurry infusion (n = 10), distinct foci of neonatal hepatocytes were observed to repopulate the parenchyma of the scaffold. The presence of cholangiocytes was verified by CK-7 positivity. Quantitative albumin measurement from the grafts showed increasing albumin levels after seven days of perfusion. Graft albumin production was higher than that observed in traditional cell culture. This data shows that rat liver scaffolds support human cell ingrowth. The scaffold likewise supported the engraftment and survival of neonatal rat liver cell slurry. Recellularization of liver scaffolds thus presents a promising model for functional liver engineering. |
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| فهرسة مساهمة: | Keywords: bioengineering; decellularization; liver; organ scaffold; rat; recellularization |
| المشرفين على المادة: | 0 (Albumins) 9007-49-2 (DNA) |
| تواريخ الأحداث: | Date Created: 20161229 Date Completed: 20171113 Latest Revision: 20181113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC5323036 |
| DOI: | 10.1080/15476278.2016.1276146 |
| PMID: | 28029279 |
| قاعدة البيانات: | MEDLINE |
PDF full text from Publisher | تدمد: | 1555-8592 |
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| DOI: | 10.1080/15476278.2016.1276146 |