دورية أكاديمية
The MAP kinase MAPKLK1 is essential to Trypanosoma brucei proliferation and regulates proteins involved in mRNA metabolism.
| العنوان: | The MAP kinase MAPKLK1 is essential to Trypanosoma brucei proliferation and regulates proteins involved in mRNA metabolism. |
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| المؤلفون: | Batista M; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil; Mass Spectrometry Facility RPT02H, Carlos Chagas Institute, Fiocruz, Parana, Brazil., Kugeratski FG; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil., de Paula Lima CV; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil., Probst CM; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil., Kessler RL; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil., de Godoy LM; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil., Krieger MA; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil., Marchini FK; Functional Genomics Laboratory, Carlos Chagas Institute, Fiocruz, Curitiba, Parana, Brazil; Mass Spectrometry Facility RPT02H, Carlos Chagas Institute, Fiocruz, Parana, Brazil. Electronic address: fabricio.marchini@fiocruz.br. |
| المصدر: | Journal of proteomics [J Proteomics] 2017 Feb 10; Vol. 154, pp. 118-127. Date of Electronic Publication: 2016 Dec 27. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101475056 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7737 (Electronic) Linking ISSN: 18743919 NLM ISO Abbreviation: J Proteomics Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier |
| مواضيع طبية MeSH: | Protein Kinases/*physiology , Protozoan Proteins/*analysis , Trypanosoma brucei brucei/*chemistry, Cell Proliferation ; Gene Expression Regulation ; Gene Silencing ; Phosphoproteins/analysis ; Phosphorylation ; Protein Kinases/genetics ; Protein Kinases/metabolism ; Proteomics/methods ; RNA, Messenger/metabolism ; RNA, Protozoan/metabolism ; Trypanosoma brucei brucei/cytology ; Trypanosoma brucei brucei/physiology |
| مستخلص: | Protein phosphorylation and dephosphorylation events regulate many cellular processes. The identification of all phosphorylation sites and their association to a respective protein kinase or phosphatase is a challenging and crucial step to have a deeper understanding of the effects of signaling networks on cells. Pathogenic trypanosomatids have a large number of protein kinases and phosphatases in comparison to other organisms, which reinforces the relevance of the phosphorylation process in these early eukaryotes, nevertheless little is known about protein phosphorylation in these protozoa. In this context, the role of a MAP kinase-like kinase (MAPKLK1), observed to be essential to proliferation of procyclic Trypanosoma brucei, was studied. After silencing MAPKLK1 expression by RNAi, the cells were evaluated by SILAC MS-based proteomics and RNA-Seq. We identified 1756 phosphorylation sites of which 384 were not previously described in T. brucei. Despite being essential, few modulations were observed at the phosphorylation patterns and gene expression levels of MAPKLK1 knockdown. These indirect targets and potential substrates of MAPKLK1 are related to key cellular processes enriched to mRNA processing and stability control. Significance: The field of cell signaling is a promising topic of study for trypanosomatids, since little is known about this topic and the gene expression regulation occurs at post-transcriptional level. In this sense, the present work increases the knowledge on protein phosphorylation process in Trypanosoma brucei. We depleted one MAP kinase (MAPKLK1) of T. brucei and evaluated the effects on the cell. We showed that MAPKLK1 is essential to the cell, while few modulations on phosphoproteome, proteome and transcriptome are observed with its depletion. Although in low number, the changes in phosphoproteome were significant, presenting possible substrate candidates of MAPKLK1 and indirect targets related to mRNA processing and stability control, metabolic pathways, among others. This result provides insights in the phosphorylation network of T. brucei, a model organism that impacts human and animal health. (Copyright © 2016. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: MAP kinase; Mass spectrometry; Phosphorylation; Proteomics; Transcriptomics; Trypanosoma brucei |
| المشرفين على المادة: | 0 (Phosphoproteins) 0 (Protozoan Proteins) 0 (RNA, Messenger) 0 (RNA, Protozoan) EC 2.7.- (Protein Kinases) |
| تواريخ الأحداث: | Date Created: 20170101 Date Completed: 20171206 Latest Revision: 20180118 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jprot.2016.12.011 |
| PMID: | 28039027 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1876-7737 |
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| DOI: | 10.1016/j.jprot.2016.12.011 |