دورية أكاديمية
أعرض في EDS

Molecular Modeling and Phylogeny of the Krüppel-like Factor 4 (cKLF4) Protein from the Arabian Camel, Camelus dromedarius .

التفاصيل البيبلوغرافية
العنوان: Molecular Modeling and Phylogeny of the Krüppel-like Factor 4 (cKLF4) Protein from the Arabian Camel, Camelus dromedarius .
المؤلفون: Alawad AO; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Alharbi SN; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Alhazzaa OA; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Alagrafi FS; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Alkhrayef MN; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Alhamdan ZA; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Alenazi AD; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., Hammad M; National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.; SAAD Research and Development Center, Clinical Research Laboratory and Radiation Oncology, SAAD Specialist Hospital, Al Khobar, Kingdom of Saudi Arabia., Alyahya SA; National Center for biotechnology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia., AlJohi HA; National Center for Genomic Technology, King Abdulaziz City for Science and Technology, Riyadh, Kingdom of Saudi Arabia., Alanazi IO; National Center for Genomic Technology, King Abdulaziz City for Science and Technology, Riyadh, Kingdom of Saudi Arabia.
المصدر: Bioinformatics and biology insights [Bioinform Biol Insights] 2016 Dec 21; Vol. 10, pp. 291-300. Date of Electronic Publication: 2016 Dec 21 (Print Publication: 2016).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: SAGE Publications Country of Publication: United States NLM ID: 101467187 Publication Model: eCollection Cited Medium: Print ISSN: 1177-9322 (Print) Linking ISSN: 11779322 NLM ISO Abbreviation: Bioinform Biol Insights Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: <2016- > : Thousand Oaks, CA : SAGE Publications
Original Publication: Auckland, New Zealand : Libertas Academica
مستخلص: Krüppel-like factor 4 (KLF4) is a pluripotency transcription factor that helps in generating induced pluripotent stem cells (iPSCs). We sequenced for the first time the full coding sequence of Camelus dromedarius KLF4 (cKLF4), which is also known as the Arabian camel. Bioinformatics analysis revealed the molecular weight and the isoelectric point of cKLF4 protein to be 53.043 kDa and 8.74, respectively. The predicted cKLF4 protein sequence shows high identity with some other species as follows: 98% with Bactrian camel and 89% with alpaca KLF4 proteins. A three-dimensional (3D) structure was built based on the available crystal structure of the Mus musculus KLF4 (mKLF4) of 82 residues (PDB: 2 WBS) and by predicting 400 residues using bioinformatics software. The comparison confirms the presence of the zinc finger domains in cKLF4 protein. Phylogenetic analysis showed that KLF4 from the Arabian camel is grouped with the Bactrian camel, alpaca, cattle, and pig. This study will help in the annotation of KLF4 protein and in generating camel-induced pluripotent stem cells (CiPSCs).
Competing Interests: Authors disclose no potential conflicts of interest.
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فهرسة مساهمة: Keywords: 3D structure; Camelus dromedarius; KLF4; camel-induced pluripotent stem cells (CiPSCs); pluripotency; sequencing
تواريخ الأحداث: Date Created: 20170105 Latest Revision: 20201001
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5179146
DOI: 10.4137/BBI.S40782
PMID: 28050127
قاعدة البيانات: MEDLINE
الوصف
تدمد:1177-9322
DOI:10.4137/BBI.S40782